Difference between revisions of "O2k-Fluo LED2-Module"
From Bioblast
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# [[Fluorescence-Control_Unit#Control_of_LED-intensity|Control of LED-intensity]] | # [[Fluorescence-Control_Unit#Control_of_LED-intensity|Control of LED-intensity]] | ||
# [[Fluorescence-Control_Unit#Amplification|Amplification]] | # [[Fluorescence-Control_Unit#Amplification|Amplification]] | ||
=== Application specific settings == | |||
{| class="wikitable" | |||
|- | |||
! Application !!Sensor!! Filter set !! Light intensity (LED current) !!Light intensity (switch position) | |||
|- | |||
| [[Amplex® UltraRed]]||[[Fluorescence-Sensor Green]] || AmR || 2 mA || 4 | |||
|- | |||
| [[Safranin]] ||[[Fluorescence-Sensor Blue]]|| Saf || 1 mA || 3 | |||
|- | |||
| [[Magnesium green]]||[[Fluorescence-Sensor Blue]]|| MgG / CaG || 1 mA || 3 | |||
|- | |||
| [[Calcium green]]||[[Fluorescence-Sensor Blue]]|| MgG / CaG || || | |||
|} | |||
=== Calibration === | === Calibration === | ||
=== DatLab-Analysis === | === DatLab-Analysis === |
Revision as of 15:33, 3 May 2012
O2k-Fluo LED2-Module
Description | The O2k-Fluorescence LED2-Module is an amperometric add-on module to the O2k-Core, adding a new dimension to HRR. Optical sensors are inserted through the front window of the O2k-glass chambers, for measurement of hydrogen peroxide production (Amplex red), ATP production (Magnesium green), mt-membrane potential (Safranin), Ca2+ (Calcium green), and numerous other applications open for O2k-user innovation.
The O2k-Fluorescence LED2-Module consists of optical sensors for both O2k-Chambers (LEDs for green and blue excitation), optical filters, Fluorescence-Control Unit for regulation of light intensity, data input into the O2k-Main Unit, and the updated DatLab software. |
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Product ID | 12100-01 |
Type | O2k, O2k-Module, MultiSensor, Catalogue |
Link | O2k-Fluorescence@OROBOROS, Oxygraph-2k |
Image |
The O2k-Fluorescence LED2-Module consists of
The query description has an empty condition.
News
Delivery
The first series of the O2k-Fluorescence LED2-Module will be completed by April 2012, and can be delivered in April/May 2012.
O2k-Fluorometry Workshop: March 2012
- O2k-Fluorometry Workshop from 15 to 16 March 2012, Innsbruck, Austria.
O2k-Guide: O2k-Fluorescence LED2-Module
As an innovation in our 'open innovation' approach, the Manual for the O2k-Fluorescence LED2-Module evolves as a guided tour through the O2k-Catalogue: O2k-Fluorescence LED2-Module.
Setup of the O2k-Fluorescence LED2-Module
Electronic settings
= Application specific settings
Application | Sensor | Filter set | Light intensity (LED current) | Light intensity (switch position) |
---|---|---|---|---|
Amplex® UltraRed | Fluorescence-Sensor Green | AmR | 2 mA | 4 |
Safranin | Fluorescence-Sensor Blue | Saf | 1 mA | 3 |
Magnesium green | Fluorescence-Sensor Blue | MgG / CaG | 1 mA | 3 |
Calcium green | Fluorescence-Sensor Blue | MgG / CaG |
Calibration
DatLab-Analysis
References
- Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2012) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (Hemiscyllum ocellatum). J Comp Physiol B 182: 91-100.
- Hunter FW, Wanga J, Patela R, Hsua H-L, Hickey AJ, Haya MP, Wilson WR (2012) Homologous recombination repair-dependent cytotoxicity of the benzotriazine di-N-oxide CEN-209: Comparison with other hypoxia-activated prodrugs. Biochem Pharmacol 83: 574–585.
- Fasching M, Harrison DK, Tretter L, Gnaiger E (2011) Combination of high-resolution respirometry and fluorometry for continuous monitoring of hydrogen peroxide production by mitochondria with resolution in the nanomolar range. Abstract Berlin.
- Sumbalova Z, Harrison DK, Gradl P, Fasching M, Gnaiger E (2011) Mitochondrial membrane potential, coupling control, H2O2 production, and the upper limit of mitochondrial performance. Abstract Kagoshima.
See also
MitoPedia methods: Fluorometry