Difference between revisions of "P-L net OXPHOS capacity"

Revision as of 19:12, 7 November 2020

high-resolution terminology - matching measurements at high-resolution

P-L net OXPHOS capacity

Description

The free OXPHOS capacity, P-L, is the OXPHOS capacity corrected for LEAK respiration, P-L. P-L is the scope for ADP stimulation, the respiratory capacity potentially available for phosphorylation of ADP to ATP. Oxygen consumption in the OXPHOS state, therefore, is partitioned into the free OXPHOS capacity, ≈P, strictly coupled to phosphorylation, P», and nonphosphorylating LEAK respiration, L_P, compensating for proton leaks, slip and cation cycling: P = P-L+L_P. It is frequently assumed that LEAK respiration L as measured in the LEAK state, overestimates the LEAK component of respiration, L_P, as measured in the OXPHOS state, particularly if the protonmotive force is not adjusted to equivalent levels in L and L_P. However, if the LEAK component increases with enzyme turnover during P, the low enzyme turnover during L may counteract the effect of the higher pmF.

Abbreviation: P-L

Reference: Gnaiger 2020 MitoPathways

Communicated by Gnaiger E (2014-08-09) last update 2020-11-07.

The Blue Book 2020

Coupling control states for P-L

» Respiratory state

Reference state, Z_X: OXPHOS capacity, P = P´-ROX
Background state, Y_X: LEAK respiration, L = L´-ROX
Metabolic control variable, X=Z_X-Y_X: Scope of ADP stimulation, free OXPHOS capacity, ≈P = P-L

Flux control efficiency

» Flux control efficiency

Coupling control efficiency, 1-Y_X/Z_X: P-L coupling control efficiency, OXPHOS-coupling efficiency: j_P-L = (P-L)/P = 1-L/P

Compare

Free ET capacity, E-L

netOXPHOS control ratio, (P-L)/E

ET-coupling efficiency, E-L control efficiency: j_E-L = (E-L)/E = 1-L/E

Intact cells: Free ROUTINE activity, ≈R = R-L

@@ Line 1: / Line 1: @@
 {{MitoPedia
-|abbr=''≈P''
+|abbr=''P-L''
-|description=[[Image:P-L.jpg|50 px|Free OXPHOS capacity]] The '''free OXPHOS capacity''', ''≈P'', is the [[OXPHOS capacity]] corrected for [[LEAK respiration]], ''≈P'' = ''P-L''. ''≈P'' is the scope for ADP stimulation, the respiratory capacity potentially available for phosphorylation of ADP to ATP. Oxygen consumption in the OXPHOS state, therefore, is partitioned into the free OXPHOS capacity, ''≈P'', strictly coupled to phosphorylation, ''~P'', and nonphosphorylating LEAK respiration, ''L<sub>P</sub>'', compensating for proton leaks, slip and cation cycling: ''P'' = ''≈P''+''L<sub>P</sub>''. It is frequently assumed that [[LEAK respiration]], ''L'', as measured in the LEAK state, overestimates the LEAK component of respiration, ''L<sub>P</sub>'', as measured in the OXPHOS state, particularly if the protonmotive force is not adjusted to equivalent levels in ''L'' and ''L<sub>P</sub>''. However, if the LEAK component increases with enzyme turnover during ''P'', the low enzyme turnover during ''L'' may counteract the effect of the higher Δ''p''<sub>mt</sub>.
+|description=[[Image:P-L.jpg|50 px|Free OXPHOS capacity]] The '''free OXPHOS capacity''', ''P-L'', is the [[OXPHOS capacity]] corrected for [[LEAK respiration]], ''P-L''. ''P-L'' is the scope for ADP stimulation, the respiratory capacity potentially available for phosphorylation of ADP to ATP. Oxygen consumption in the OXPHOS state, therefore, is partitioned into the free OXPHOS capacity, ''≈P'', strictly coupled to phosphorylation, ''P»'', and nonphosphorylating LEAK respiration, ''L<sub>P</sub>'', compensating for proton leaks, slip and cation cycling: ''P'' = ''P-L''+''L<sub>P</sub>''. It is frequently assumed that [[LEAK respiration]] ''L'' as measured in the LEAK state, overestimates the LEAK component of respiration, ''L<sub>P</sub>'', as measured in the OXPHOS state, particularly if the protonmotive force is not adjusted to equivalent levels in ''L'' and ''L<sub>P</sub>''. However, if the LEAK component increases with enzyme turnover during ''P'', the low enzyme turnover during ''L'' may counteract the effect of the higher ''pmF''.
 |info=[[Gnaiger 2020 MitoPathways]]
 }}
-{{MitoPedia concepts
+Communicated by [[Gnaiger E]] (2014-08-09) last update 2020-11-07.
-|mitopedia concept=Respiratory state, Recommended
+[[File:EPL-free and excess.jpg|right|240px|thumb|[[Gnaiger 2020 MitoPathways |The Blue Book 2020]]]]
-}}
-{{MitoPedia methods}}
-{{MitoPedia O2k and high-resolution respirometry}}
-{{MitoPedia topics
-|mitopedia topic=EAGLE
-}}
-Communicated by [[Gnaiger E]] 2014-08-09, edited 2015-01-19.
-[[File:EPL-free and excess.jpg|right|240px|thumb|[[Gnaiger 2014 MitoPathways |The Blue Book 2014]]: Fig. 2.4.]]
-== Coupling control states for ''≈P'' ==
+== Coupling control states for ''P-L'' ==
 ::» [[Respiratory state]]
 * [[Reference state]], ''Z<sub>X</sub>'': [[Image:P.jpg|link=OXPHOS capacity|OXPHOS capacity]] [[OXPHOS capacity]], ''P'' = ''P´''-ROX
@@ Line 22: / Line 14: @@
-== Flux control factor ==
+== Flux control efficiency ==
-::» [[Flux control factor]], ''FCF''
+::» [[Flux control efficiency]]
-* [[Coupling control factor]], 1-''Y<sub>X</sub>/Z<sub>X</sub>'': [[Image:j--P.jpg|50 px|link=OXPHOS-coupling efficiency |OXPHOS-coupling efficiency]] ''P-L'' coupling control factor, [[OXPHOS-coupling efficiency]]: ''j<sub>≈P</sub>'' = ''≈P/P'' =(''P-L'')/''P'' = 1-''L/P''
+* [[Coupling control efficiency]], 1-''Y<sub>X</sub>/Z<sub>X</sub>'': [[Image:j--P.jpg|50 px|link=OXPHOS-coupling efficiency |OXPHOS-coupling efficiency]] ''P-L'' coupling control efficiency, [[OXPHOS-coupling efficiency]]: ''j<sub>P-L</sub>'' = (''P-L'')/''P'' = 1-''L/P''
 == Compare ==
-:: [[Image:E-L.jpg|50 px|link=Free ET capacity |Free ET capacity]] [[Free ET capacity]], ''≈E'' = ''E-L''
+:: [[Image:E-L.jpg|50 px|link=Free ET capacity |Free ET capacity]] [[Free ET capacity]], ''E-L''
-:: [[Image:NetP over E.jpg|60 px|link=NetOXPHOS control ratio |netOXPHOS control ratio]] [[netOXPHOS control ratio]], ''≈P/E'' control ratio: ''≈P/E'' = (''P-L'')/''E''
+:: [[Image:NetP over E.jpg|60 px|link=NetOXPHOS control ratio |netOXPHOS control ratio]] [[netOXPHOS control ratio]], (''P-L'')/''E''
-:: [[Image:j--E.jpg|50 px|link=ET-coupling efficiency |ET-coupling efficiency]] [[ET-coupling efficiency]], ''E-L'' control factor: ''j<sub>≈E</sub>'' = ''≈E/E'' = (''E-L'')/''E'' = 1-''L/E''
+:: [[Image:j--E.jpg|50 px|link=ET-coupling efficiency |ET-coupling efficiency]] [[ET-coupling efficiency]], ''E-L'' control efficiency: ''j<sub>E-L</sub>'' = (''E-L'')/''E'' = 1-''L/E''
 * Intact cells: [[Image:R-L.jpg|50 px|link=Free ROUTINE activity |Free ROUTINE activity]] [[Free ROUTINE activity]], ''≈R'' = ''R-L''