Difference between revisions of "Resorufin"
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'''Resorufin''' is a fluorescence probe used in various biological assays. Among others, it is the product obtained in the [[Horseradish_peroxidase| Horseradish peroxidase-catalyzed assay using [[Amplex Red]] for the measurement of H<sub>2</sub>O<sub>2</sub> production. | {{MitoPedia | ||
|abbr=Res | |||
|description='''Resorufin''' is a fluorescence probe used in various biological assays. Among others, it is the product obtained in the [[Horseradish_peroxidase| Horseradish peroxidase]]-catalyzed assay using [[Amplex_red| Amplex Red]] for the measurement of [[Hydrogen_peroxide| H<sub>2</sub>O<sub>2</sub>]] production. | |||
}} | |||
{{MitoPedia methods | {{MitoPedia methods | ||
|mitopedia method=Fluorometry | |mitopedia method=Fluorometry | ||
}} | }} | ||
__TOC__ | |||
== Preparation of | == Preparation of resorufin solutions == | ||
Source: Sigma 424455 | :::: Source: Sigma 424455 | ||
=== Storage solution === | === Storage solution === | ||
Prepare a 1.2 mM storage solution of resorufin by adding 13.5 mg resorufin in 50 | :::: Prepare a 1.2 mM storage solution of resorufin by adding 13.5 mg resorufin in 50 mL of a 10 mM KOH solution and store aliquots of 2 mL of this storage solution at -20 °C. | ||
=== Stock solution === | === Stock solution === | ||
Prior to experiments thaw 2 | :::: Prior to experiments thaw 2 mL storage solution, pipette 1.666 mL into a 25 mL volumetric flask and add 10 mM KOH to 25 mL to obtain a stock solution of 80 µM resorufin. | ||
{{Keywords: H2O2}} | |||
Latest revision as of 00:24, 18 February 2020
- high-resolution terminology - matching measurements at high-resolution
Resorufin
Description
Resorufin is a fluorescence probe used in various biological assays. Among others, it is the product obtained in the Horseradish peroxidase-catalyzed assay using Amplex Red for the measurement of H2O2 production.
Abbreviation: Res
MitoPedia methods:
Fluorometry
Preparation of resorufin solutions
- Source: Sigma 424455
Storage solution
- Prepare a 1.2 mM storage solution of resorufin by adding 13.5 mg resorufin in 50 mL of a 10 mM KOH solution and store aliquots of 2 mL of this storage solution at -20 °C.
Stock solution
- Prior to experiments thaw 2 mL storage solution, pipette 1.666 mL into a 25 mL volumetric flask and add 10 mM KOH to 25 mL to obtain a stock solution of 80 µM resorufin.
- Bioblast links: Hydrogen peroxide - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Specific
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