Difference between revisions of "SUIT-003 Ce1;ce2SD;ce3U;ce4Rot;ce5Ama"
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{{MitoPedia | {{MitoPedia | ||
|abbr=CCP_R,1S,2U- | |abbr=CCP_R,1S,2U-3Rot,4Ama | ||
|description=[[File:CCP | |description=[[File:CCP(S)01.jpg|200px]] | ||
|info=[[Steinlechner-Maran 1997 Transplantation]] | |info=[[Steinlechner-Maran 1997 Transplantation]] | ||
}} | }} | ||
{{MitoPedia concepts | {{MitoPedia concepts | ||
|mitopedia concept=SUIT protocol | |mitopedia concept=SUIT protocol, SUIT B | ||
}} | }} | ||
{{MitoPedia methods}} | |||
{{MitoPedia O2k and high-resolution respirometry}} | |||
{{MitoPedia topics}} | |||
[[File:CCP(S)01(R,E).jpg|right|200px]] | |||
::: '''[[Categories of SUIT protocols |SUIT-catg]]:''' CCP(S) | ::: '''[[Categories of SUIT protocols |SUIT-catg]]:''' CCP(S) | ||
::: '''[[SUIT protocol pattern]]:''' complex [[coupling control protocol]] (CCP) | ::: '''[[SUIT protocol pattern]]:''' complex [[coupling control protocol]] (CCP) |
Revision as of 04:50, 18 September 2016
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 Ce1;ce2SD;ce3U;ce4Rot;ce5Ama
Description
Abbreviation: CCP_R,1S,2U-3Rot,4Ama
Reference: Steinlechner-Maran 1997 Transplantation
MitoPedia concepts:
SUIT protocol,
SUIT B
- SUIT-catg: CCP(S)
- SUIT protocol pattern: complex coupling control protocol (CCP)
- Mark names in DatLab: CCP(S)01
- DatLab-Excel template: in prep.: CCP(S)01.xlsx
Coupling control protocol
Step | Respiratory state | Pathway control | Comment |
---|---|---|---|
R | ROUTINE | ce | Intact cells, ce, are in the ROUTINE state of respiration. pce without substrate are in a state of ROX. |
1S | R+SL | ROUTINE (R) + S (SL) | pce are in state SL. ADP should be added to stimulate pce to the OXPHOS state; see CCP(S)02. |
2U | E+SE | ETS (E) + S (SE) | ce and pce are in the ETS state. |
3Rot | ROX+SE | ROX+S (SE) | ce are in the ROX state, pce are in the ETS state. |
4Ama | ROXAma | ROX |