Difference between revisions of "SUIT-023"
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== Strengths and limitations == | == Strengths and limitations == | ||
:::* SUIT-023 provides an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-non-resistant mitochondrial respiration due to the activity of the cytochrome c pathway on cells on [[ROUTINE]] | :::* SUIT-023 provides an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-non-resistant mitochondrial respiration due to the activity of the cytochrome c pathway on cells on [[ROUTINE]]. To complement the study with the estimation of the contribution of the [[alternative oxidase]] pathway to respiration, it must be carried out in parallel to [[SUIT-022]]. | ||
:::+ This protocol can be used with many type of cells, although optimal concentrations of inhibitors have to be optimised. | :::+ This protocol can be used with many type of cells, although optimal concentrations of inhibitors have to be optimised. | ||
:::+ Short duration of experiment | :::+ Short duration of experiment | ||
Revision as of 10:54, 9 April 2019
- high-resolution terminology - matching measurements at high-resolution
SUIT-023
Description
Reference: A: Determination of the respiration due to the cytochrome c pathway. 
»Versions
O2k-Application: O2
- SUIT-category:
- SUIT protocol pattern: linear ce1;ce2KCN;ce3SHAM
The SUIT-023 is designed to provide an estimation of the cyanide-and antimycin A-non-resistant mitochondrial respiration on cells on ROUTINE. To obtain a complete estimation of the contribution of both the alternative oxidase and the cytochrome c pathways, both SUIT-022 O2 ce D051 and SUIT-023 O2 ce D053 must be carried out in parallel.
Communicated by Iglesias-Gonzalez J, Huete-Ortega M and Gnaiger E (last update 2019-04-09)
Specific SUIT protocols
SUIT-023 O2 ce D053
- SUIT-023 O2 ce D053 for intact microalgal cells (ce)
Strengths and limitations
- SUIT-023 provides an estimation of the cyanide-and antimycin A-non-resistant mitochondrial respiration due to the activity of the cytochrome c pathway on cells on ROUTINE. To complement the study with the estimation of the contribution of the alternative oxidase pathway to respiration, it must be carried out in parallel to SUIT-022.
- + This protocol can be used with many type of cells, although optimal concentrations of inhibitors have to be optimised.
- + Short duration of experiment
- - Estimation of the linear coupling control is not done
- - The use of cyanide requires a careful and in deep cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.
Compare SUIT protocols
References
MitoPedia concepts: MiP concept, SUIT protocol, Recommended
MitoPedia methods:
Respirometry
