Difference between revisions of "SUIT-023"

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SUIT-023

Description

Reference: A: Determination of the respiration due to the cytochrome c pathway »Versions

O2k-Application: O2

SUIT-category: ce

SUIT protocol pattern: linear ce1;ce2KCN;ce3SHAM

The SUIT-023 is designed to provide an estimation of the mitochondrial respiration in cells due to the cytochrome c pathway, which is non-resistant to cyanide-and antimycin A inhibition. This protocol must be complemented with the study in parallel of the mitochondrial respiration due to the alternative oxidase pathway (see SUIT-022).

Communicated by Huete-Ortega M and Gnaiger E (last update 2019-04-09)

Specific SUIT protocols

SUIT-023 O2 ce D053

SUIT-023 O2 ce D053 for intact microalgal cells (ce)

Template:SUIT-023

Strengths and limitations

SUIT-023 provides an estimation of the cyanide-and antimycin A-non-resistant mitochondrial respiration due to the activity of the cytochrome c on cells on ROUTINE. To complement the study with the estimation of the mitochondrial respiration due to the alternative oxidase pathway, it must be carried out in parallel to SUIT-022.

+ This protocol can be used with many type of cells, although optimal concentrations of inhibitors have to be optimised.

+ Short duration of experiment

- Estimation of the linear coupling control is not done

- The use of cyanide requires a careful and in deep cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.

Compare SUIT protocols

SUIT-022 O2 ce D051 for microalgal intact cells

References

MitoPedia concepts: MiP concept, SUIT protocol, Recommended

MitoPedia methods: Respirometry

@@ Line 1: / Line 1: @@
 {{MitoPedia
-|info='''A:''' Determination of the respiration due to the cytochrome c pathway. [[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/6/64/SUIT-023.pdf|Bioblast pdf]] »[http://wiki.oroboros.at/index.php/File:SUIT-023.pdf Versions]
+|info='''A: Determination of the respiration due to the cytochrome ''c'' pathway''' [[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/6/64/SUIT-023.pdf|Bioblast pdf]] »[http://wiki.oroboros.at/index.php/File:SUIT-023.pdf Versions]
 |application=O2
 }}
-::: '''[[Categories of SUIT protocols |SUIT-category]]:'''
+::: '''[[Categories of SUIT protocols |SUIT-category]]:''' ce
 ::: '''[[SUIT protocol pattern]]:''' linear ce1;ce2KCN;ce3SHAM
-The SUIT-023 is designed to provide an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-non-resistant mitochondrial respiration on cells on [[ROUTINE]]. To obtain a complete estimation of the contribution of both the [[alternative oxidase]] and the cytochrome c pathways, both [[SUIT-022 O2 ce D051]] and [[SUIT-023 O2 ce D053]] must be carried out in parallel.
+The SUIT-023 is designed to provide an estimation of the mitochondrial respiration in cells due to the cytochrome ''c'' pathway, which is non-resistant to [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]] inhibition. This protocol must be complemented with the study in parallel of the mitochondrial respiration due to the [[alternative oxidase]] pathway (see [[SUIT-022]]).
 __TOC__
-  Communicated by [[Iglesias-Gonzalez J]], [[Huete-Ortega M]] and [[Gnaiger E]] (last update 2019-04-09)
+  Communicated by [[Huete-Ortega M]] and [[Gnaiger E]] (last update 2019-04-09)
 == Specific SUIT protocols ==
@@ Line 18: / Line 18: @@
 *  [[SUIT-023 O2 ce D053]] for intact microalgal cells (ce)
-{{Template:SUIT-022}}
+{{Template:SUIT-023}}
 == Strengths and limitations ==
-:::* SUIT-023 provides an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-non-resistant mitochondrial respiration due to the activity of the cytochrome c pathway on cells on [[ROUTINE]]. To complement the study with the estimation of the contribution of the [[alternative oxidase]] pathway to respiration, it must be carried out in parallel to [[SUIT-022]].
+:::* SUIT-023 provides an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-non-resistant mitochondrial respiration due to the activity of the cytochrome ''c'' on cells on [[ROUTINE]]. To complement the study with the estimation of the mitochondrial respiration due to the [[alternative oxidase]] pathway, it must be carried out in parallel to [[SUIT-022]].
 :::+ This protocol can be used with many type of cells, although optimal concentrations of inhibitors have to be optimised.
 :::+ Short duration of experiment
@@ Line 30: / Line 30: @@
 == Compare SUIT protocols ==
+::::*[[SUIT-022 O2 ce D051]] for [[microalgal]] intact cells