- high-resolution terminology - matching measurements at high-resolution
SUIT-023
Description
Reference: A: Determination of the respiration due to the cytochrome c pathway 
»Versions
O2k-Application: O2
- SUIT-category: ce
- SUIT protocol pattern: linear ce1;ce2KCN;ce3SHAM
The SUIT-023 is designed to provide an estimation of the mitochondrial respiration in cells due to the cytochrome c pathway, which is non-resistant to cyanide-and antimycin A inhibition. This protocol must be complemented with the study in parallel of the mitochondrial respiration due to the alternative oxidase pathway (see SUIT-022).
Communicated by Huete-Ortega M and Gnaiger E (last update 2019-04-09)
Specific SUIT protocols
SUIT-023 O2 ce D053
- SUIT-023 O2 ce D053 for intact microalgal cells (ce)
Strengths and limitations
- SUIT-023 provides an estimation of the cyanide-and antimycin A-non-resistant mitochondrial respiration due to the activity of the cytochrome c on cells on ROUTINE. To complement the study with the estimation of the mitochondrial respiration due to the alternative oxidase pathway, it must be carried out in parallel to SUIT-022.
- + This protocol can be used with many type of cells, although optimal concentrations of inhibitors have to be optimised.
- + Short duration of experiment
- - Estimation of the linear coupling control is not done
- - The use of cyanide requires a careful and in deep cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.
Compare SUIT protocols
- SUIT-022 O2 ce D051 for microalgal intact cells
References
MitoPedia concepts: MiP concept, SUIT protocol, Recommended
MitoPedia methods:
Respirometry
