Difference between revisions of "Talk:O2k-Open Support alert"
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::::::: While running your experiment, especially during [[uncoupler titrations]], it is important to decrease the slope smoothing in order to have a faster visualization of the titrations effect on respiration. - »[[Smoothing#DatLab_oxygen_flux:_performance_and_data_analysis|Slope smoothing]]« | ::::::: While running your experiment, especially during [[uncoupler titrations]], it is important to decrease the slope smoothing in order to have a faster visualization of the titrations effect on respiration. - »[[Smoothing#DatLab_oxygen_flux:_performance_and_data_analysis|Slope smoothing]]« | ||
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] ''' | :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Did you know that you can use the [[TIP2k-Module|Titration-Injection microPump (TIP2k)]] to perform an instrumental O<sub>2</sub> background on the [[O2k-sV-Module]]?''' | ||
::::::: By adjusting the [[Dithionite|dithionite]] concentration to 2.5 mM, the [[MiPNet14.06 Instrumental O2 background|instrumental O<sub>2</sub> background]] can be performed with specific settings for the [[O2k-sV-Module]]. - »[[O2k-sV-Module]]« | ::::::: By adjusting the [[Dithionite|dithionite]] concentration to 2.5 mM, the [[MiPNet14.06 Instrumental O2 background|instrumental O<sub>2</sub> background]] can be performed with specific settings for the [[O2k-sV-Module]]. - »[[O2k-sV-Module]]« | ||
Revision as of 10:05, 5 March 2020
Do you perform the leaky chamber test after chamber assembly?
- After chamber reassembly you might experience a small leaky chamber only identified by a leaky chamber test- »Leaky chamber test«
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Do you change the slope smoothing to 20 while running experiments with biological sample?
- While running your experiment, especially during uncoupler titrations, it is important to decrease the slope smoothing in order to have a faster visualization of the titrations effect on respiration. - »Slope smoothing«
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Did you know that you can use the Titration-Injection microPump (TIP2k) to perform an instrumental O2 background on the O2k-sV-Module?
- By adjusting the dithionite concentration to 2.5 mM, the instrumental O2 background can be performed with specific settings for the O2k-sV-Module. - »O2k-sV-Module«
