Template:Next oroboros ecosystem agenda

2020 Week 41

Oct-05 Mo

Oct-06 Tu - ÖGMBT Life Science Tuesdays

Fasting promotes functional changes in liver mitochondria: Is it time to reconsider using fasted animals for liver mitochondria isolation?

from the O2k-Network »BR Sao Paulo Kowaltowski AJ«

Menezes-Filho SL, Amigo I, Luévano-Martínez LA, Kowaltowski AJ (2018) Fasting promotes functional changes in liver mitochondria. Biochim Biophys Acta Bioenerg 1860:129-35. »Bioblast link«

Oct-07 We

Background H₂O₂ calibration: what is it and why perform it when using Amplex UltraRed assay?

Background H₂O₂ calibration refers to a set of H₂O₂ titrations conducted after addition of respiration medium, DTPA, HRP, SOD and Amplex UltraRed to the O2k-chamber but before sample addition. This step is required 1) for the calculation of the theoretical chemical background fluorescence in our Excel analysis template, and 2) for the calculation of the sensitivity of the AmR assay, which serves as a quality control of the assay.
- »Background H₂O₂ calibration«
- communicated by »Komlodi Timea« and »Schmitt Sabine«

Oct-09 Fr

»Long Night of Research 2020 Innsbruck AT«

2020 Week 42

Oct-12 Mo http://www.metabesity2020.com/

If using the Amplex UltraRed assay, when should the background H₂O₂ calibration be performed?

Background H₂O₂ calibration needs to be performed for each individual instrumental setting (O2k, fluorescence sensor and chamber), if a new stock solution of DTPA, HRP, SOD, Amplex UltraRed, or H₂O₂ is prepared, or if a different respiration medium is used. As the H₂O₂ stock solution needs to be freshly prepared, it is recommended to perform a background H₂O₂ calibration before each experiment and at a minimum once per experimental day.
- »How to find the background H₂O₂ calibration protocol in DatLab « select “AmR calibration” for the background H₂O₂ calibration protocol
- communicated by »Komlodi Timea« and »Schmitt Sabine«