Difference between revisions of "Template:SUIT-002"
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{{Template:SUIT M_low}} | |||
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| ([[F]]) | | ([[F]]) | ||
| FAO | | FAO | ||
| {{Template:SUIT F}} | | | ||
{{Template:SUIT F}} | |||
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| [[F]] | | [[F]] | ||
| FAO | | FAO | ||
| {{Template:SUIT F}} {{Template:SUIT OXPHOS}} {{Template:SUIT c}} | | | ||
{{Template:SUIT F}} {{Template:SUIT OXPHOS}} {{Template:SUIT c}} | |||
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| [[F]] | | [[F]] | ||
| FAO | | FAO | ||
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{{Template:SUIT M_high}} | |||
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| [[FN]] | | [[FN]] | ||
| FAO&CI | | FAO&CI | ||
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{{Template:SUIT P}} | |||
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| [[FN]] | | [[FN]] | ||
| FAO&CI | | FAO&CI | ||
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{{Template:SUIT G}} | |||
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| [[FNS]] | | [[FNS]] | ||
| FAO&CI&II | | FAO&CI&II | ||
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{{Template:SUIT FNS}} | |||
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| [[FNSGp]] | | [[FNSGp]] | ||
| FAO&CI&II&GpDH | | FAO&CI&II&GpDH | ||
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{{Template:SUIT FNSGp}} | |||
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| [[FNSGp]] | | [[FNSGp]] | ||
| FAO&CI&II&GpDH | | FAO&CI&II&GpDH | ||
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| [[SGp]] | | [[SGp]] | ||
| CII&GpDH | | CII&GpDH | ||
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{{Template:SUIT CIV}} | {{Template:SUIT CIV}} | ||
Revision as of 14:51, 11 January 2019
| Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
|---|---|---|---|---|
| 1D | ROX | ADP is added to stimulate consumption of endogenous fuel-substrates.
Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated either after inhibition of CIII (e.g. antimycin A, myxothiazol), CIV (e.g. Cyanide) or in the absence of endogenous fuel-substrates. Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration. | ||
| 2M.1 |
Low concentration of malate, typically 0.1 mM, does not saturate the N-pathway; but saturates the F-pathway. | |||
| 3Oct | OctP | (F) | FAO |
Respiratory stimulation of the FAO-pathway, F, by fatty acid, FA, in the presence of malate, M. Malate is a type N substrate (N), required for the F-pathway. The FA concentration has to be optimized to saturate the F-pathway, without inhibiting or uncoupling respiration. |
| 3c | OctMcP | F | FAO |
Respiratory stimulation of the FAO-pathway, F, by fatty acid, FA, in the presence of malate, M. Malate is a type N substrate (N), required for the F-pathway. The FA concentration has to be optimized to saturate the F-pathway, without inhibiting or uncoupling respiration. OXPHOS capacity P (with saturating [ADP]), active OXPHOS state. Addition of cytochrome c yields a test for integrity of the mtOM (cytochrome c control efficiency). Stimulation by added cytochrome c would indicate an injury of the mtOM and limitation of respiration in the preceding state without added c due to loss of cytochrome c. Typically, cytochrome c is added immediately after the earliest ADP-activation step (OXPHOS capacity P with saturating [ADP]). |
| 4M2 | OctMP | F | FAO |
High concentration of malate, typically 2 mM, saturates the N-pathway. |
| 5P | OctPMP | FN | FAO&CI | |
| 6G | OctPGMP | FN | FAO&CI | |
| 7S | OctPGMSP | FNS | FAO&CI&II |
Respiratory stimulation by simultaneous action of the F-pathway, N-pathway, and S-pathway, with convergent electron flow in the FNS-pathway for reconstitution of TCA cycle function and additive or inhibitory effect of F. |
| 8Gp | OctPGMSGpP | FNSGp | FAO&CI&II&GpDH |
Respiratory stimulation by simultaneous action of fatty acid (F), type N substrates (N), succinate (S), and glycerophosphate with convergent electron flow in the FNSGp-pathway to the Q-junction. |
| 9U | OctPGMSGpE | FNSGp | FAO&CI&II&GpDH | |
| 10Rot | SGpE | SGp | CII&GpDH | |
| 11Ama | ROX |
| Step | Respiratory state | Pathway control | ET-Complex | Comment |
|---|---|---|---|---|
| ## AsTm | AsTmE | CIV | CIV | |
| ## Azd | CHB |
