Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "Template:SUIT-011"

From Bioblast
Line 16: Line 16:
| GM<sub>''L''</sub> or GM_L: Glutamate & malate, N-LEAK respiration, N<sub>''L''</sub>
| GM<sub>''L''</sub> or GM_L: Glutamate & malate, N-LEAK respiration, N<sub>''L''</sub>


NADH-linked substrates (type N; CI-linked pathway to Q). Non-phosphorylating resting state (LEAK state); ''L''<sub>n</sub> in the absence of ADP, ATP, AMP (no adenylates).
{{Template:SUIT N}} {{Template:SUIT L n}}


|-
|-
Line 25: Line 25:
| GM<sub>''P''</sub> or GM_P: N-OXPHOS capacity, N<sub>''P''</sub>  
| GM<sub>''P''</sub> or GM_P: N-OXPHOS capacity, N<sub>''P''</sub>  


OXPHOS capacity, ''P'' (with saturating [ADP]), with NADH-linked substrates.
{{Template:SUIT N}} {{Template:SUIT OXPHOS}}


|-
|-
Line 34: Line 34:
| GMc<sub>''P''</sub> or GMc_P: Cytochrome c test for quality control
| GMc<sub>''P''</sub> or GMc_P: Cytochrome c test for quality control


Addition of cytochrome ''c'' yields a test for integrity of the [[mtOM]]. Stimulation by added cytochrome ''c'' would indicate an injury of the mtOM and limitation of respiration in state GM<sub>''P''</sub> due to loss of cytochrome ''c'' (''P'', OXPHOS capacity with type N substrates). Cytochrome ''c'' is added immediately after the earliest ADP-activation step.
{{Template:SUIT N}} {{Template:SUIT OXPHOS}} {{Template:SUIT c}}


|-
|-
Line 43: Line 43:
| GMS<sub>''P''</sub> or GMS_P: NS-OXPHOS capacity, NS<sub>''P''</sub>
| GMS<sub>''P''</sub> or GMS_P: NS-OXPHOS capacity, NS<sub>''P''</sub>


Respiratory stimulation by further addition of succinate, S, to type N substrates, with convergent electron flow in the NS-pathway (CI<small>&</small>II-linked pathway to the Q-junction) for reconstitution of TCA cycle function, in the coupled state as an estimate of OXPHOS-capacity, ''P''.
{{Template:SUIT N}} & {{Template:SUIT S}} {{Template:SUIT NS}} {{Template:SUIT OXPHOS}}


|-
|-
Line 52: Line 52:
| GMS<sub>''E''</sub> or GMS_E: NS-ET capacity, NS<sub>''E''</sub>
| GMS<sub>''E''</sub> or GMS_E: NS-ET capacity, NS<sub>''E''</sub>


Uncoupler titration (avoiding inhibition by high uncoupler concentrations) to obtain electron transfer (ET) capacity (noncoupled ET-state), as a test for limitation of OXPHOS-capacity by the phosphorylation system (ANT, ATP synthase, phosphate transporter) relative to ET-capacity, E.
{{Template:SUIT NS}} {{Template:SUIT U*}}


|-
|-
Line 61: Line 61:
| S<sub>''E''</sub> or S_E: S-ET capacity
| S<sub>''E''</sub> or S_E: S-ET capacity


S-pathway ET-capacity after blocking CI with rotenone.
{{Template:SUIT Rot}} {{Template:SUIT ET}}


|-
|-
Line 70: Line 70:
| ROX: residual oxygen consumption
| ROX: residual oxygen consumption


''Rox'' is due to oxidative side reactions, estimated after addition of Antimycin A (inhibitor of CIII). ''Rox'' is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt).
{{Template:SUIT Ama}}
|}
|}


{{Template:SUIT CIV}}
{{Template:SUIT CIV}}

Revision as of 13:12, 11 January 2019

1GM;2D;3S;4U;5Rot-.png

Step Respiratory state Pathway control ET-Complex entry into Q-junction Comment
1GM GML N CI GML or GM_L: Glutamate & malate, N-LEAK respiration, NL

NADH-linked substrates (type N-pathway to Q). Template:SUIT L n

2D GMP N CI GMP or GM_P: N-OXPHOS capacity, NP

NADH-linked substrates (type N-pathway to Q). OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.

D(c) GMcP N CI GMcP or GMc_P: Cytochrome c test for quality control

NADH-linked substrates (type N-pathway to Q). OXPHOS capacity P (with saturating [ADP]), active OXPHOS state. Addition of cytochrome c yields a test for integrity of the mtOM (cytochrome c control efficiency). Stimulation by added cytochrome c would indicate an injury of the mtOM and limitation of respiration in the preceding state without added c due to loss of cytochrome c. Typically, cytochrome c is added immediately after the earliest ADP-activation step (OXPHOS capacity P with saturating [ADP]).

3S GMSP NS CI&II GMSP or GMS_P: NS-OXPHOS capacity, NSP

NADH-linked substrates (type N-pathway to Q). & Succinate, S ( type S-pathway to Q). Respiratory stimulation by simultaneous action of type N substrates & succinate, with convergent electron flow in the NS-pathway for reconstitution of TCA cycle function. OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.

4U GMSE NS CI&II GMSE or GMS_E: NS-ET capacity, NSE

Respiratory stimulation by simultaneous action of type N substrates & succinate, with convergent electron flow in the NS-pathway for reconstitution of TCA cycle function. Uncoupler titration (avoiding inhibition by high uncoupler concentrations) to obtain electron transfer (ET) capacity E (noncoupled ET-state). Test for limitation of OXPHOS capacity P by the phosphorylation system (ANT, ATP synthase, phosphate transporter) relative to ET capacity E in mt-preparations: E-P control efficiency and E-L coupling efficiency. In living cells: E-R control efficiency and E-L coupling efficiency.

5Rot SE S CII SE or S_E: S-ET capacity

Succinate pathway control state (S-pathway) after inhibiting CI with rotenone, which also inhibits the F-pathway. Noncoupled electron transfer state, ET state, with ET capacity E.

6Ama ROX ROX: residual oxygen consumption

Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated after addition of antimycin A (inhibitor of CIII). Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt).

Step Respiratory state Pathway control ET-Complex Comment
## AsTm AsTmE CIV CIV
## Azd CHB
Retrieved from "https://wiki.oroboros.at/index.php?title=Template:SUIT-011&oldid=168919"
Powered by MediaWiki
Powered by Semantic MediaWiki