Noncoupled respiration

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Noncoupled respiration


E.jpg Noncoupled respiration is distinguished from general (pharmacological or mechanical) uncoupled respiration, to give a label to an effort to reach the state of maximum uncoupler-activated respiration without inhibiting respiration. Noncoupled respiration, therefore, yields an estimate of ET-capacity. Experimentally uncoupled respiration may fail to yield an estimate of ET-capacity, due to inhibition of respiration above optimum uncoupler concentrations or insufficient stimulation by sub-optimal uncoupler concentrations. Optimum uncoupler concentrations for evaluation of (noncoupled) ET-capacity require inhibitor titrations (Steinlechner-Maran 1996 Am J Physiol Cell Physiol; Huetter 2004 Biochem J; Gnaiger 2008 POS).

Noncoupled respiration is maximum electron flow in an open-transmembrane proton circuit mode of operation (see ET-capacity).

Abbreviation: E

Reference: Gnaiger 2014 MitoPathways, MitoPedia: Respiratory states

MitoPedia concepts: Respiratory state, Recommended 

MitoPedia methods: Respirometry 

MitoPedia topics: EAGLE 

Contributed by Gnaiger E 2010-08-15, edited 2015-01-19.

Is respiration uncoupled - noncoupled - dyscoupled?

» More details: Uncoupler

Biochemical coupling efficiency: from 0 to <1

» More details: OXPHOS coupling efficiency and ET-coupling efficiency

Related MitoPedia pages

  • Electron transfer-pathway, ET-pathway
» Electron transfer-pathway
» Q-junction
  • Electron transfer-pathway states
» Electron transfer-pathway state
  • Coupling control state E
E.jpg ET-capacity
» Noncoupled respiration
» Is respiration uncoupled - noncoupled - dyscoupled?