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Noncoupled respiration
Description
Noncoupled respiration is distinguished from general (pharmacological or mechanical) uncoupled respiration, to give a label to an effort to reach the state of maximum uncoupler-activated respiration without inhibiting respiration. Noncoupled respiration, therefore, yields an estimate of ET-capacity. Experimentally uncoupled respiration may fail to yield an estimate of ET-capacity, due to inhibition of respiration above optimum uncoupler concentrations or insufficient stimulation by sub-optimal uncoupler concentrations. Optimum uncoupler concentrations for evaluation of (noncoupled) ET-capacity require inhibitor titrations (Steinlechner-Maran 1996 Am J Physiol Cell Physiol; Huetter 2004 Biochem J; Gnaiger 2008 POS).
Noncoupled respiration is maximum electron flow in an open-transmembrane proton circuit mode of operation (see ET-capacity).
Abbreviation: E
Reference: Gnaiger 2014 MitoPathways, MitoPedia: Respiratory states
MitoPedia concepts:
Respiratory state,
Recommended
MitoPedia methods:
Respirometry
MitoPedia topics:
EAGLE
Contributed by Gnaiger E 2010-08-15, edited 2015-01-19.
Is respiration uncoupled - noncoupled - dyscoupled?
- » More details: Uncoupler
Biochemical coupling efficiency: from 0 to <1
- » More details: OXPHOS coupling efficiency and ET-coupling efficiency
Related MitoPedia pages
- Electron transfer-pathway, ET-pathway
- Electron transfer-pathway states
- Coupling control state E
- ET-capacity
- » Noncoupled respiration
- » Is respiration uncoupled - noncoupled - dyscoupled?
