Difference between revisions of "Template talk:Next oroboros ecosystem agenda"
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== Bioblast alert 2021 == | == Bioblast alert 2021 == | ||
Prep for circular: | Prep for circular: | ||
PhotoBiology | PhotoBiology | ||
:{| | :{| | ||
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- Ā»'''[[PhotoBiology]]'''Ā« | - Ā»'''[[PhotoBiology]]'''Ā« | ||
|} | |} | ||
For Mito-Kit-CII Bioblast Circular (cell-permeable succinate, or toxicology?): | |||
:{| | |||
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] | |||
|'''A cell-permeable succinate prodrug can alleviate N-methyl carbamate-induced Complex I dysfunction'''<br> | |||
:from the O2k-Network Ā»[[US PA Philadelphia Kilbaugh T]]Ā«, Ā»[[US PA Philadelphia Jang DH]]Ā« and Ā»[[SE Lund Elmer E]]Ā« | |||
*Janowska JI, Piel S, Saliba N, Kim CD, Jang DH, Karlsson M, Kilbaugh TJ, Ehinger JK (2020) Mitochondrial respiratory chain Complex I dysfunction induced by N-methyl carbamate ex vivo can be alleviated with a cell-permeable succinate prodrug carbamate toxicity and treatment. Toxicol In Vitro 65:104794. [[Janowska 2020 Toxicol In Vitro|Ā»Bioblast linkĀ«]] | |||
|} | |||
:{| | |||
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] | |||
|''' Measuring succinate pathway (S-pathway) in living cells '''<br> | |||
Using [[MitoKit-CII]] ā cell permeable succinate and malonate, the [[Succinate pathway|S-pathway]] can be measuredĀ <br> | |||
- Ā»'''[[SUIT-003 O2 ce D060]]'''Ā«<br> | |||
- communicated by Ā»[[Cecatto Cristiane]]Ā« and Ā»[[Komlodi Timea]]Ā« | |||
|} | |||
:{| | |||
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]] | |||
|'''Cell-permeable succinate can restore statin-inhibited mitochondrial respiration ''' <br> | |||
:from the O2k-Network Ā»[[RO Timisoara Muntean DM]]Ā« and Ā»[[SE Lund Elmer E]]Ā« | |||
* Avram VF, Chamkha I, Ć
sander-Frostner E, Ehinger JK, Timar RZ, Hansson MJ, Muntean DM, ElmĆ©r E (2021) Cell-permeable succinate rescues mitochondrial respiration in cellular models of statin toxicity. Int J Mol Sci 22:424. [[Avram 2021 Int J Mol Sci|Ā»Bioblast linkĀ«]] | |||
|} | |||
For Prostate Cancer Bioblast Circular (September?): | |||
:{| | |||
| width="100" align="center" |[[File:TRANSMIT Acronym.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] | |||
|''' Succinate anaplerosis enhances the malignant potential of prostate cancer cells.'''<br> | |||
:from the O2k-Network Ā»[[AT Innsbruck Gnaiger E]]Ā« and Ā»[[FR Bordeaux Rossignol R]]Ā« | |||
*Bastos Sant'Anna Silva AC, Perez-Valencia JA, Sciacovelli M, Lalou C, Sarlak S, Tronci L, Nikitopoulou E, Meszaros AT, Frezza C, Rossignol R, Gnaiger E, Klocker H (2021) Succinate anaplerosis has an onco-driving potential in prostate cancer cells. Cancers 13:1727.[[Bastos Sant'Anna Silva 2021 Cancers|Ā»Bioblast linkĀ«]] | |||
|} | |||
:{| | |||
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] | |||
|''' pH-regulated succinate uptake in prostate cancer''' <br> | |||
:from the O2k-Network Ā»[[US PA Philadelphia Orynbayeva Z]]Ā« | |||
*Zhunussova A, Sen B, Friedman L, Tuleukhanov S, Brooks AD, Sensenig R, Orynbayeva Z (2015) Tumor microenvironment promotes dicarboxylic acid carrier-mediated transport of succinate to fuel prostate cancer mitochondria. Am J Cancer Res 5:1665-79. [[Zhunussova 2015 Am J Cancer Res|Ā»Bioblast linkĀ«]] | |||
|} | |||
:{| | |||
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] | |||
|'''Succinate: a key regulator of mitochondrial function and HIF-1Ī± upregulation in prostate cancer''' <br> | |||
:from the O2k-Network Ā»[[AT Innsbruck Oroboros]]Ā«, Ā»[[AT Innsbruck Gnaiger E]]Ā« | |||
*Weber A, Klocker H, Oberacher H, Gnaiger E, Neuwirt H, Sampson N, Eder IE (2018) Succinate accumulation is associated with a shift of mitochondrial respiratory control and HIF-1Ī± upregulation in PTEN negative prostate cancer cells. Int J Mol Sci 19:2129. [[Weber 2018 Int J Mol Sci|Ā»Bioblast linkĀ«]] | |||
|} | |||
:{| | |||
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]] | |||
|'''Ā Remodeling of OXPHOS in prostate cancer to potentially damaging mtDNA mutations and differential expression of mt-genes ''' <br> | |||
: from the O2k-Network Ā»[[AT Innsbruck Gnaiger E]]Ā« and Ā»[[AT Innsbruck Oroboros]]Ā« <br> | |||
* Schƶpf Bernd, Weissensteiner Hansi, SchƤfer Georg, Fazzini Federica, Charoentong Pornpimol, Naschberger Andreas, Rupp Bernhard, Fendt Liane, Bukur Valesca, Giese Irina, Sorn Patrick, SantāAnna-Silva Ana Carolina, Iglesias-Gonzalez Javier, Sahin Ugur, Kronenberg Florian, Gnaiger Erich, Klocker Helmut (2020) OXPHOS remodeling in high-grade prostate cancer involves mtDNA mutations and increased succinate oxidation. Nat Commun 11:1487.Ā [[Schoepf 2020 Nat Commun|Ā»Bioblast linkĀ«]] | |||
|} | |||
== MitoPedia alert == | == MitoPedia alert == | ||
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==Ā O2k-Open Support alert == | ==Ā O2k-Open Support alert == |
Revision as of 12:36, 30 April 2021
- Antunes Diana (talk) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:
{| | logos | title in bold <br> - link |}
e.g.,
Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?
- Ā»Instrumental backgroundĀ« and Ā»TIP2k Filter PapersĀ«
- See the scheme:
Bioblast alert 2021
Prep for circular: PhotoBiology
Exploring photosynthesis and dark respiration in the microalgae Chlamydomonas reinhardtii
Measurement of oxygen dependence with High-Resolution PhotoRespirometry:
- Went N, Di Marcello M, Gnaiger E (2021) Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry. MitoFit Prep 2021.5. doi:10.26124/mitofit:2021-0005
How can you monitor O2 flux in response to light?
The PB-Module of the upcoming the NextGen-O2k will enable experiments in PhotoBiology.
- Ā»PB-ModuleĀ« and Ā»PhotoBiologyĀ«
Photosynthesis efficiency is reduced by 25 % in plants and algae due the metabolic process called photorespiration.
- Ā»PhotorespirationĀ«
Would you like to measure the O2 flux that occurs during photosynthesis using the O2k and conduct experiments about primary productivity in aquatic ecosystems? Soon you will be able to with the PB-Module that we are developing as part of the H2020 NextGen-O2k project.
- Ā»PhotoBiologyĀ«
For Mito-Kit-CII Bioblast Circular (cell-permeable succinate, or toxicology?):
A cell-permeable succinate prodrug can alleviate N-methyl carbamate-induced Complex I dysfunction
- from the O2k-Network Ā»US PA Philadelphia Kilbaugh TĀ«, Ā»US PA Philadelphia Jang DHĀ« and Ā»SE Lund Elmer EĀ«
- Janowska JI, Piel S, Saliba N, Kim CD, Jang DH, Karlsson M, Kilbaugh TJ, Ehinger JK (2020) Mitochondrial respiratory chain Complex I dysfunction induced by N-methyl carbamate ex vivo can be alleviated with a cell-permeable succinate prodrug carbamate toxicity and treatment. Toxicol In Vitro 65:104794. Ā»Bioblast linkĀ«
Measuring succinate pathway (S-pathway) in living cells
Using MitoKit-CII ā cell permeable succinate and malonate, the S-pathway can be measured
- Ā»SUIT-003 O2 ce D060Ā«
- communicated by Ā»Cecatto CristianeĀ« and Ā»Komlodi TimeaĀ«
Cell-permeable succinate can restore statin-inhibited mitochondrial respiration
- from the O2k-Network Ā»RO Timisoara Muntean DMĀ« and Ā»SE Lund Elmer EĀ«
- Avram VF, Chamkha I, Ć sander-Frostner E, Ehinger JK, Timar RZ, Hansson MJ, Muntean DM, ElmĆ©r E (2021) Cell-permeable succinate rescues mitochondrial respiration in cellular models of statin toxicity. Int J Mol Sci 22:424. Ā»Bioblast linkĀ«
For Prostate Cancer Bioblast Circular (September?):
Succinate anaplerosis enhances the malignant potential of prostate cancer cells.
- from the O2k-Network Ā»AT Innsbruck Gnaiger EĀ« and Ā»FR Bordeaux Rossignol RĀ«
- Bastos Sant'Anna Silva AC, Perez-Valencia JA, Sciacovelli M, Lalou C, Sarlak S, Tronci L, Nikitopoulou E, Meszaros AT, Frezza C, Rossignol R, Gnaiger E, Klocker H (2021) Succinate anaplerosis has an onco-driving potential in prostate cancer cells. Cancers 13:1727.Ā»Bioblast linkĀ«
pH-regulated succinate uptake in prostate cancer
- from the O2k-Network Ā»US PA Philadelphia Orynbayeva ZĀ«
- Zhunussova A, Sen B, Friedman L, Tuleukhanov S, Brooks AD, Sensenig R, Orynbayeva Z (2015) Tumor microenvironment promotes dicarboxylic acid carrier-mediated transport of succinate to fuel prostate cancer mitochondria. Am J Cancer Res 5:1665-79. Ā»Bioblast linkĀ«
Succinate: a key regulator of mitochondrial function and HIF-1Ī± upregulation in prostate cancer
- from the O2k-Network Ā»AT Innsbruck OroborosĀ«, Ā»AT Innsbruck Gnaiger EĀ«
- Weber A, Klocker H, Oberacher H, Gnaiger E, Neuwirt H, Sampson N, Eder IE (2018) Succinate accumulation is associated with a shift of mitochondrial respiratory control and HIF-1Ī± upregulation in PTEN negative prostate cancer cells. Int J Mol Sci 19:2129. Ā»Bioblast linkĀ«
Remodeling of OXPHOS in prostate cancer to potentially damaging mtDNA mutations and differential expression of mt-genes
- from the O2k-Network Ā»AT Innsbruck Gnaiger EĀ« and Ā»AT Innsbruck OroborosĀ«
- Schƶpf Bernd, Weissensteiner Hansi, SchƤfer Georg, Fazzini Federica, Charoentong Pornpimol, Naschberger Andreas, Rupp Bernhard, Fendt Liane, Bukur Valesca, Giese Irina, Sorn Patrick, SantāAnna-Silva Ana Carolina, Iglesias-Gonzalez Javier, Sahin Ugur, Kronenberg Florian, Gnaiger Erich, Klocker Helmut (2020) OXPHOS remodeling in high-grade prostate cancer involves mtDNA mutations and increased succinate oxidation. Nat Commun 11:1487. Ā»Bioblast linkĀ«
- from the O2k-Network Ā»AT Innsbruck Gnaiger EĀ« and Ā»AT Innsbruck OroborosĀ«
MitoPedia alert
Next
O2k-Brief alert
Next
O2k-Publications alert
Author names of O2k-Publications/ Brief for the Agenda vs Bioblast
- 1.Oroboros Ecosystem Agenda: SOP: Surname with long version and First name with short version. Please, see: Template alerts.
- Example: Oroboros Ecosystem Agenda example
- This means that we have to edit manually the author names within the O2k-Publications/ Brief alerts (i.e., Antunes Diana (from Bioblast) to Antunes D (to Agenda entries)) if they are long or copy from the gray box.
- 2.Bioblast: SOP: [1] ā examples are available using both Surname and First name with long version (e.g., Antunes Diana). If in Bioblast the authors are with short surname in the links please update it to complete surname, guidelines are below.
- 1.Oroboros Ecosystem Agenda: SOP: Surname with long version and First name with short version. Please, see: Template alerts.
Guidelines to update a reference
- When creating an O2k-Publication/Brief alert, it is the responsibility of the author to check if the reference is accordingly to the correct guidelines:
- Correct format for a person called āFirst Name Surnameā:
- In the gray box should be āSurname FNā - From where references to be used as O2k-Publication/Brief alerts are copied from.
- In the links should be ā Surname First Nā
- How to edit:
- A) Check if all authors with profiles in Bioblast (blue links) have their profiles with full name. If they have, proceed to next step (B). If not, you need to move it following the steps āHow to move a profileā below.
- B) Click in āEdit with formā.
- C) The gray box is edited in āReferenceā and the links are changed in āAuthor(s)ā. Change it accordingly and click in āsave pageā at the end of the page.
- How to edit:
How to move a profile (instructions sent by Marija):
So letās take for example my page: Beno M [2]
- As you can see there is a redirect from Beno Marija to Beno M. What has to be done is to switch the content of those pages. Here are the steps:
- 1. Go to e.g. āBeno Mā on edit
- 2. Click on āMoveā
- 3. Change the new title to the full name of the person. In my case it will be Beno M to Beno Marija
- As you can see there is a redirect from Beno Marija to Beno M. What has to be done is to switch the content of those pages. Here are the steps:
- 4. Now it should look like that:
- 5. Click on āMoveā and leave all the ticked boxes
- 6.Done
- After that you can continue in step B.
- If you have any doubt you can contact Cristiane, Marija or Mario.
Next
Does prenatal hypoxia cause sex-dependent effects of mitochondrial function?
- from the O2k-Network Ā»UK Manchester Galli GLĀ«
- Hellgren KT, Premanandhan H, Quinn CJ, Trafford AW, Galli GLJ (2020) Sex-dependent effects of developmental hypoxia on cardiac mitochondria from adult murine offspring . Free Radic Biol Med [Epub ahead of print]. Ā»Bioblast linkĀ«
O2k-Open Support alert
- While in Home Office: Gnaigere (talk) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda analysis-linked O2k-Open Support cases during this time, and collect real-time operation cases for future releases.
Next
What is the advantage of using flux control ratios (FCRs)?
FCRs provide information on coupling and substrate control of mitochondrial respiration independent of (i) mt-content in cells or tissues, (ii) purification of isolated mitochondrial preparations, and (iii) assay conditions for determination of tissue mass or mt-markers.
- Ā»Flux control ratioĀ«
Under discussion
Removed for correction
- Nov-30 Mo
Noisy oxygen signal? Check the OroboPOS and OroboPOS-Connector to clean electrical connections.
Contaminating grease, salt crystals and moisture can be removed with a dry paper cloth or a paper cloth moistened with water and then absolute ethanol.
- Ā»Cleaning the electrical connectionsĀ«
- communicated by Ā»Schmitt SabineĀ« and Ā»Komlodi TimeaĀ«
WGT recommended not to use water for cleaning. Erich introduced water for cleaning to remove salt crystals.
Do you perform the leaky chamber test after chamber assembly?
An unnoticed leak from the chamber may affect your data, therefore we recommend performing the Leaky chamber test - especially after chamber reassembly.
- Ā»Leaky chamber testĀ« and Ā» https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assemblyĀ«
O2k-Open Support alert circulars
In preparation
For discussion
Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 (if the specific alert section is yet to be attributed)
Controlled stability of the MitoKit-CII based on HPLC and LCMS analysis: No degradation of MitoKit-CII compounds MitoKit-CII/Succinate-nv (NV118) and MitoKit-CII/Malonate-nv (NV161) for following storage conditions: - Proven -80 Ā°C solid stability for more than 3 years (3 years 7 months)
- Proven -20 Ā°C solid stability for 1 year
- Proven DMSO stability at room temperature for 1 week
- Proven DMSO stability at -20 Ā°C for 6 months (ongoing)
2021
- 2021-01-06 https://www.jstor.org/stable/1706346?seq=1
Postponed events
- Pre-Conference O2k-Workshop Ā»MiPNet25.11 IOC148 Riga LV Ā« March 2021?
- Ā»6th EU-Cardioprotection Meeting 2020 Riga LVĀ« (Oct 7-9)- March 2021?
- Ā»APS2020 Chicago USĀ«
- Abstracts deadline 10th Int. CeBiTec Research Conference Advances in Industrial Biotechnology: Prospects and challenges for the development of algal biotechnology
- Notification to oral and poster presenters - Ā»MitoEAGLE Summit Obergurgl 2020Ā« (2020 Nov 04-07) and Ā»MiPschool Obergurgl 2020Ā« (2020 Nov 09-11)
- Bioenergetics Vienna 2020
- IOC Schroecken AT
- Registration and late Abstract Deadline - Ā»MitoEAGLE Summit Obergurgl 2020Ā« and Ā»MiPschool Obergurgl 2020Ā«
- Ā»36th Congress Czech Nutrition Society 2020 Hradec Kralove CZĀ« (Sep 10-12)