Difference between revisions of "Three-electrode system"

Latest revision as of 09:07, 15 September 2021

high-resolution terminology - matching measurements at high-resolution

Three-electrode system

Description

A three-electrode system is the setup used in the Q-Sensor, which is an integral part of the Q-Module. This system is used in voltammetry (including cyclic voltammetry) to study the current as a function of the applied potential using three different electrodes: 1) the working electrode 2) the reference electrode, and 3) the counter electrode. In the Q-Sensor, the working or detecting electrode is a glassy carbon (GC) electrode that is set to a given potential and makes contact with the analyte. The potential of the working electrode is controlled by the constant potential of the a silver/silver chloride (Ag/AgCl) reference electrode, which does not pass any current. The applied potential on the surface of the GC should be sufficient to either oxidize reduced analyte (in this case Coenzyme Q) or to reduce oxidized analyte. Thus, the counter electrode is a platinum electrode (Pt) that passes a current to counter these redox events by completing the circuit that is rate-limited by electron transfer on the GC. To determine the reduced Q fraction the GC electrode is set at the oxidation peak potential, which can be determined with cyclic voltammetry.

The Q-Sensor is an integral part of the Q-Module and NextGen-O2k project

The Q-Module allows for monitoring of the redox state of electron transfer-reactive coenzyme Q at the Q-junction using the specific Q-Stoppers with the integrated three-electrode system and the modified electronics of the NextGen-O2k. Cyclic voltammetry is used for quality control and for defining the polarization voltage applied during Q-redox measurements.

Reference:

Komlódi T, Cardoso LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. Bioenerg Commun 2021.3. https://doi.org/10.26124/bec:2021-0003

Communicated by Komlodi T, Cardoso LHD 2020-07-28

Bioblast links: Q - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

Coenzyme Q

» Coenzyme Q

» Quinone, Ubiquinone Q; oxidized

» Quinol, Ubiquinol QH₂; reduced

» Semiquinone

» Coenzyme Q2

» Q-redox state

» Q-pools

Mitochondrial pathways, respiratory Complexes, and Q

» Q-cycle

» Q-junction

» Convergent electron flow

» NS-pathway

» FNS

» FNSGp

» N-pathway

» Reverse electron flow from CII to CI

» CI

» Rotenone

» Amytal

» Piericidin

» S-pathway

» CII

» Malonate

» F-pathway

» CETF, Electron-transferring flavoprotein complex

» Gp-pathway

» CGpDH, Glycerophosphate dehydrogenase complex

» CIII

» Myxothiazol

» Choline dehydrogenase

» Dihydro-orotate dehydrogenase

NextGen-O2k and Q-Module

» NextGen-O2k

» Q-Module

» Q-Sensor

» Cyclic voltammetry

» Three-electrode system

General

» Categories of SUIT protocols

» Electron transfer pathway

» Electron-transfer-pathway state

» F-junction

» N-junction

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 {{MitoPedia
 |abbr=
-|description='''Three-electrode system''' is the three-electrode setup of the [[Q-Sensor]]. In general it is applied in voltammetry to study the current as a function of the applied potential with three different electrodes: 1.) working electrode which makes contact with analyte, 2.) reference electrode which has a known potential and controls the current of the working electrode, but it does not pass any current, and 3.) counter electrode which maintains a constant potential while passing current to counter redox events at the working electrode. In the [[Q-Sensor]], which is and integral part of the [[Q-Module]], the working or detecting electrode is a glassy carbon (GC) electrode  which is set to a given potential versus a silver/silver chloride (Ag/AgCl) reference electrode. The applied potential on the surface of the GC should be sufficient to either oxidise reduced analyte (in our case [[Coenzyme Q]]) or to reduce oxidised CoQ. The third electrode is a platinum electrode (Pt) that acts as a counter electrode to complete the circuit that is rate-limited by electron transfer on the GC. To determine the Q redox ratio the GC electrode is set at the oxidation peak potential.
+|description= A '''three-electrode system''' is the setup used in the [[Q-Sensor]], which is an integral part of the [[Q-Module]]. This system is used in voltammetry (including [[cyclic voltammetry]]) to study the current as a function of the applied potential using three different electrodes:       1) the working electrode 2) the reference electrode, and 3) the counter electrode. In the [[Q-Sensor]], the working or detecting electrode is a glassy carbon (GC) electrode that is set to a given potential and makes contact with the analyte. The potential of the working electrode is controlled by the constant potential of the a silver/silver chloride (Ag/AgCl) reference electrode, which does not pass any current.  The applied potential on the surface of the GC should be sufficient to either oxidize reduced analyte (in this case [[Coenzyme Q]]) or to reduce oxidized analyte. Thus, the counter electrode is a platinum electrode (Pt) that passes a current to counter these redox events by completing the circuit that is rate-limited by electron transfer on the GC. To determine the reduced Q fraction the GC electrode is set at the oxidation peak potential, which can be determined with [[cyclic voltammetry]].
-|info=::::
 }}
-==  The [[Q-Sensor]] as an integral part of the [[Q-Module]] is part of the [[NextGen-O2k]] project==
+==  The [[Q-Sensor]] is an integral part of the [[Q-Module]] and [[NextGen-O2k]] project==
-In the [[NextGen-O2k]] project we are working on developing the Q-Module for the new series of our [[Startup_O2k-Respirometer| O2k-Respirometer]], the NextGen-O2k. The Q-Module allows us to simultaneously monitor the O<sub>2</sub> flux and Q redox state of the [[Q-junction]] using the specific Q-Stoppers with the integrated three-electrode system and with the modified electronics in the O2k housing.
+{{Template:Q-Module}}
-  Communicated by [[Komlodi T]] 2020-07-09
+  Communicated by [[Komlodi T]], [[Cardoso LHD]] 2020-07-28
-{{NextGen-O2k}}
-{{Labeling
+{{NextGen-O2k H2020-support}}
-|additional=MitoPedia:NextGen-O2k
+{{Template:Keywords: Q}}
-}}