Difference between revisions of "MiPNet19.15 IOC97"
m (moved MiPNet19.15 IOC097 to MiPNet19.15 IOC97 over redirect) |
|||
Line 1: | Line 1: | ||
{{Mainpage OROBOROS}} | {{Mainpage OROBOROS}} | ||
{{Publication | {{Publication | ||
|title=[[Image:O2k-WorldWide.jpg|right|60px|link= | |title=[[Image:O2k-WorldWide.jpg|right|60px|link=MiPNet_Reference_Laboratories|MiPNet Reference Laboratories]] | ||
'''Lausanne CH''', 2014 Sep. 25-26 OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, '''IOC97.''' | '''Lausanne CH''', 2014 Sep. 25-26 OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, '''IOC97.''' | ||
|info=[[Media:MiPNet19.15_IOC97_2014-09_Lausanne.pdf |'''MiPNet19.15.pdf''']] | |info=[[Media:MiPNet19.15_IOC97_2014-09_Lausanne.pdf |'''MiPNet19.15.pdf''']] | ||
Line 7: | Line 7: | ||
|year=2014-09-25 | |year=2014-09-25 | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract=[[Image:O2k-Workshops.png|left|130px|link= | |abstract=[[Image:O2k-Workshops.png|left|130px|link=O2k-Workshops|O2k-Workshops]] | ||
'''97th OROBOROS O2k-Workshop on high-resolution respirometry and O2k-Fluorometry''' | '''97th OROBOROS O2k-Workshop on high-resolution respirometry and O2k-Fluorometry''' | ||
|mipnetlab=CH Lausanne Sandi C, AT Innsbruck OROBOROS | |mipnetlab=CH Lausanne Sandi C, AT Innsbruck OROBOROS | ||
Line 164: | Line 164: | ||
<big>'''Key references'''</big> | <big>'''Key references'''</big> | ||
[[Image:O2k-Publications.jpg|left|50px|link= | [[Image:O2k-Publications.jpg|left|50px|link=O2k-Publications: Topics|O2k-Publications in the MiPMap]] | ||
: Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352. [[Gnaiger_2008_POS |»Bioblast Access]] | : Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352. [[Gnaiger_2008_POS |»Bioblast Access]] | ||
:* Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58. [[Pesta 2012 Methods Mol Biol |»Bioblast Access]] | :* Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58. [[Pesta 2012 Methods Mol Biol |»Bioblast Access]] | ||
Line 170: | Line 170: | ||
<big>'''Introduction and O2k-Core Manual'''</big> | <big>'''Introduction and O2k-Core Manual'''</big> | ||
[[Image:O2k-Manual.jpg|left|30px|link= | [[Image:O2k-Manual.jpg|left|30px|link=O2k-Manual|O2k-Manual]] | ||
: [[Media:MiPNet19.01 O2k-Core Manual.pdf|'''»O2k-Core Manual.pdf''']] | : [[Media:MiPNet19.01 O2k-Core Manual.pdf|'''»O2k-Core Manual.pdf''']] | ||
<big>'''HRR and O2k-Fluorometry'''</big> | <big>'''HRR and O2k-Fluorometry'''</big> | ||
[[Image:O2k- | [[Image:O2k-Fluorometer.JPG|60px|left|link=O2k-Fluorometer|O2k-Fluorometer]] | ||
: Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542: 163-181. [[Krumschnabel 2014 Methods Enzymol |»Pubmed 24862266]] | : Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542: 163-181. [[Krumschnabel 2014 Methods Enzymol |»Pubmed 24862266]] | ||
:* »[[MiPNet17.05 O2k-Fluorescence LED2-Module |Manual: O2k- | :* »[[MiPNet17.05 O2k-Fluorescence LED2-Module |Manual: O2k-Fluo LED2-Module]] | ||
:* »[[O2k-Publications: O2k-Fluorometry|O2k-Fluorometry]]''' | :* »[[O2k-Publications: O2k-Fluorometry|O2k-Fluorometry]]''' | ||
Line 187: | Line 187: | ||
[[Image:MitoGlobal.jpg|right|80px|link= | [[Image:MitoGlobal.jpg|right|80px|link=MitoGlobal|MitoGlobal]] | ||
O2k-Workshops are listed as [[MitoGlobal Events]]. | O2k-Workshops are listed as [[MitoGlobal Events]]. | ||
[[Category:O2k-Workshops]] | [[Category:O2k-Workshops]] |
Revision as of 22:42, 2 January 2015
Lausanne CH, 2014 Sep. 25-26 OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC97. |
OROBOROS (2014-09-25) Mitochondr Physiol Network
Abstract:
97th OROBOROS O2k-Workshop on high-resolution respirometry and O2k-Fluorometry
• O2k-Network Lab: CH Lausanne Sandi C, AT Innsbruck OROBOROS
Labels:
ORO, IOC, 2014, MiPNet
» MitoPedia: Respiratory states
Lecturer
Gnaiger Erich, CEO, OROBOROS INSTRUMENTS
Programme
Programme MiPNet19.15.pdf
Thursday, September 25
09:00 – 10:30 A new Power-O2k Lab of Prof. Carmen Sandi: 4 O2k.
- Instrumental setup, chamber assembly, OroboPOS – sensor service, quality control (group Carmen Sandi).
10:30 Break – Registration of external participants
11:00 – 11:15 Erich Gnaiger:
- Welcome to external participants. OROBOROS INSTRUMENTS and perspectives of mitochondrial physiology.
11:15 – 11:30 Introduction of participants: who is who?
11:30 – 12:30 Basic and new features of DatLab 6.
- Oxygen calibration.
- Oxygen flux and instrumental tests.
12:30 Lunch
13:30 – 15:30 Experimental design.
- Cell ergometry: from intact cells to mt-preparations.
- A challenge for simultaneous measurements of respiration and mt-membrane potential: solving a puzzle.
15:30 Break
16:00 – 17:30 Titration-Injection microPump TIP2k:
- From automatic instrumental background tests to steady-state control in respiratory experiments.
17:30 – 18:00 Q&A session on HRR and OXPHOS analysis
- Design of experimental protocol - day 2.
Friday, September 26
09:00 – 10:30 Experiment
- HRR and O2k-Fluorometry – respiration and H2O2 production.
10:30 Break
11:00 – 12:30 Experiment continued.
12:30 Lunch
13:15 – 15:30 DatLab analysis.
15:30 Break
16:00 – 16:30 Trouble shooting.
16:30 – 17:30 Normalization of mt-respiration: flux control states and flux control steps.
17:30 - 18:00 Feedback – conclusions – stay connected as a MiPNet Lab.
Venue
Laboratory of Behavioral Genetics
Brain Mind Institute
Ecole Polytechnique Federale de Lausanne
Auditorium/Meeting room
CH-1015 Lausanne
Contact: Prof. Carmen Sandi, Director. Email: carmen.sandi@epfl.ch
Registration and general information
OROBOROS INSTRUMENTS
high-resolution respirometry
Schöpfstrasse 18
A-6020 Innsbruck, Austria
Tel: +43 512 566796
Fax: +43 512 566796 20
Participants
Name | Institution |
---|---|
Canto Alvarez Carlos (Thursday, Friday) | CH Lausanne Canto C: Nestlé Institute of Health Sciences SA, EPFL |
Eggimann Sandra | CH Bern Nuoffer JM: Universitätsinstitut für klinische Chemie, Inselspital Bern |
Grosse Jocelyn (Thursday) | CH Lausanne Sandi C: Brain Mind Institute, EPFL |
Hahn Dagmar | CH Bern Nuoffer JM: Universitätsinstitut für klinische Chemie, Inselspital Bern |
Hollis Fiona (Thursday afternoon and Friday) | CH Lausanne Sandi C: Brain Mind Institute, EPFL |
Huzard Damien (Thursday morning and Friday morning) | CH Lausanne Sandi C: Brain Mind Institute, EPFL |
Meng Kévin (Thursday afternoon and Friday morning) | CH Lausanne Sandi C: Brain Mind Institute, EPFL |
Kulkarni Sameer | CH Lausanne Canto C: Nestlé Institute of Health Sciences SA, EPFL |
Papilloud Aurélie (Thursday and Friday) | CH Lausanne Sandi C: Brain Mind Institute, EPFL |
Ratajczak Joanna (Thursday afternoon and Friday) | CH Lausanne Canto C: Nestlé Institute of Health Sciences SA, EPFL |
Riccio Orbicia (Thursday afternoon and Friday) | CH Lausanne Sandi C: Brain Mind Institute, EPFL |
Sandi Carmen | CH Lausanne Sandi C: Brain Mind Institute, EPFL |
Walker Sophie (Thursday) | CH Lausanne Sandi C: Brain Mind Institute, EPFL |
Zanoletti Olivia (Thursday and Friday) | CH Lausanne Sandi C: Brain Mind Institute, EPFL |
Recommended reading
Key references
- Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352. »Bioblast Access
- Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58. »Bioblast Access
Introduction and O2k-Core Manual
HRR and O2k-Fluorometry
- Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542: 163-181. »Pubmed 24862266
Mitochondrial pathways
- Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck: 80 pp. »Open Access
O2k-Workshops are listed as MitoGlobal Events.