MiPNet28.02 IOC162 Schroecken AT

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MiPNet28.02 IOC162 Schroecken AT

Publications in the MiPMap
Schroecken AT, 2023 Oct 02-07. Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC162.

Oroboros (2023-10-02) Mitochondr Physiol Network

Abstract: Oroboros O2k-Workshop on high-resolution respirometry. Schroecken, Austria (2023 October 02-07).

O2k-Network Lab: AT_Innsbruck_Oroboros


Please help us to improve the O2k-Workshops by taking a few minutes to answer the following questions: IOC162 Feedback
  • Doing the entire hands on module from start to finish was the best for really "cementing" theory and hands on application together. - Cosgrove Richard
  • Introduction to the new models available with the NextGen was immensely helpful. This workshop has been critical for implementation of new techniques and advancement of my research. - Zunica Elizabeth
  • Scientific values in addition to instrument operating training only. Hands-on training sessions are the most important and valuable. - Doan Khanh
  • I was mostly interested in the workshop for the hand on and all of the quality control, service etc. And the workshop certainly delivered.
  • I really enjoyed the opportunity to work with the instruments and to ask the team any questions I had. I also enjoyed the opportunity to work with other groups and to listen and to discuss scientific talks. I think it was really useful to see the SUIT protocols, the POS services, the background and other protocols related to the O2K. In my opinion, it was a great experience.
  • Very good and benevolent ambiance to learn and progress without any judgement. Very interesting discussions and dynamic group (staff and O2kers). - Feller Louise
  • At no point did I ever feel like I couldn’t ask a question or take more time to further dive into a topic that was presented or get extra help on any hands on module.
Question Rating (average) 1-5
In how far were your interests covered in the workshop? Please rate from 1 (not covered) to 5 (all interests covered). 4.6
Balance between practical “hands-on” work and “theoretical” explanations: Please rate from 1 (too much “hands-on”) to 5 (too much theory). 3.2
Presentation of lectures: Please rate from 1 (not understandable) to 5 (very understandable). a. in terms of content: 4.2
Presentation of lectures: Please rate from 1 (not understandable) to 5 (very understandable). b. in terms of language: 4.5
Level of tutoring during the “hands-on” work: Please rate from 1 (not enough tutoring, I felt left alone) to 5 (too much tutoring, I would have liked to work more independently). 3.9
Quality of tutoring: Please rate from 1 (poor) to 5 (excellent). 4.8
Intensity of the O2k-Course: Please rate from 1 (I would have liked to do and hear more) to 5 (I felt overwhelmed). 3
Your overall rating of the course? Please rate from 1 (poor) to 5 (excellent). 4.7

General information

This training course on the Oroboros O2k provides an introduction to DatLab 8 and applications of the TIP2k. Hands-on experiments illustrate the principle and show the unique advantages and limitations of simultaneous monitoring of oxygen concentration, mitochondrial respiration and hydrogen peroxide production. Many optimized SUIT protocols are available as DL-Protocols. The SUITbrowser helps you find the best SUIT protocol for your specific research questions. Furthermore, this workshop covers interpretation as well as calibration and instrumental service.


We would like to advise you to make sure to book flights that will allow you to arrive in Bregenz in time for the scheduled pickup.
In the case of delay, please call Oroboros Instruments Mobile: +43 677 64 79 29 17 or Oroboros Office: +43 512 566 796 (until 5:30 pm) as soon as you know you will arrive late.
If you miss the scheduled transportation you will have to take a cab (Suggestion: Ritter Transport. Phone number: +43 664 34 11 540) or the public transportation to Schroecken. If you decide to take the public transportation, you will have to take the bus via Bregenz- Egg – Bezau – Schoppernau – Schröcken Dorf. You can book the ticket here: vmbol or here: oebb. Please note that the last bus leaves Bregenz at 17:33, after that the only option to get to Schroecken is by cab.
Please be aware that you will be responsible for paying for any additional transportation costs (about € 120 for a cab/ travel time 1 h; about € 11 for the public transportation / travel time 1.5 h - 2 h). Please check with your airline to see if there are any reimbursements available in the event that you miss the organized transport and have to arrange and pay for a cab or public transportation.


  • Program found here: Bioblast pdf


Tutors and lecturers


Participant Institution
1. Francisco Miranda Miranda Francisco Consejo Superior de Investigaciones Científicas, ES
2. Louise Feller Feller Louise Université de Namur, BE
3. Maria Pino Pino Maria F US FL Orlando Sparks LM - AdventHealth, US
4. Colleen O'Reilly O'Reilly Colleen US OK Oklahoma City Miller BF - Oklahoma Medical Research Foundation, US
5. Hergert Elvira Hergert Elvira DE Cologne Pesta D - German Aerospace Center, DE
6. Philip A Kramer Kramer Philip A US NC Winston-Salem Molina AJA - Wake Forest School of Medicine, US
7. Els Van de Westerlo Van de Westerlo Els NL Nijmegen Koopman WJH - Radboud University Nijmegen Medical Centre , NL
8. Richard Cosgrove Cosgrove Richard Lilly Corporate Center, USA
9. Profile-icon-9.png Bratislava, SK
10. Khanh Doan Doan Khanh US PA Philadelphia Baur JA - University of Pennsylvania, US
11. Giuseppe Sirago Sirago Giuseppe CH Lausanne Place N - University of Lausanne, CH
12. Olga Duerrschmidt Duerrschmidt Olga DE Duesseldorf Roden M - German Diabetes Center, DE
13. Mazzen Eldeeb Eldeeb Mazzen US FL Miami Barrientos A - University of Miami, USA
14. Maria Ines Alves Alves Maria Ines Faculty of Medicine of the University of Porto, PT
15. Profile-icon-9.png Mezghani Ikram Penn State College of Medicine, USA
16. Profile-icon-9.png Zunica Elizabeth US LA Baton Rouge Noland RC - Pennington Biomedical Research Center, USA
17. Profile-icon-9.png Olivieri Enrica Universidad de Granada , ES
18. Profile-icon-9.png Latif Abdul NO_Bodo_Viswanath K - Nord University, NO
19. Profile-icon-9.png Banni Aml CZ Hradec Kralove Cervinkova Z - Charles University, CZ
20. Profile-icon-9.png César Fernández Muñoz AT Innsbruck Oroboros

O2k perspectives - abstracts

Abstracts for the O2k perspectives evenings provide participants an opportunity to present and discuss their work, including projects in progress, methodological details and conceptual perspectives.
» Abstract guidelines
 Has title
Alves 2023 Abstract IOC162Alves IN, Goncalves A, Araujo M, Leite L, Morana-Fernaz S, Morais J, Rodrigues A, Souza L, Mendes C, Silva C, Silva S, Charrua A, Falcao-Pires I (2023) Long term Ketogenic diet modulates glycemic control and physical endurance in a sex-dependent manner. Mitochondr Physiol Network 28.02
Van Doan 2023 Abstract IOC162Van Doan K, Luongo TS, Mukherjee S, Baur JA (2023) Mouse cardiac mitochondria are resilient to Nampt-dependent NAD+ los. Mitochondr Physiol Network 28.02
Eldeeb 2023 Abstract IOC162Eldeeb M, Fontanesi F (2023) Differential substrate utilization by mitochondrial respiratory supercomplexes. Mitochondr Physiol Network 28.02
Pino 2023 Abstract IOC162Pino MF, Kapar SG, Laurent NL, Erickson ML, Sparks LM (2023) Characterization of human subcutaneous adipose-derived stem cells isolated from healthy young and old individual. Mitochondr Physiol Network 28.02
Additional short presentations
  • Ondra Sobotka: Human platelets in mitochondrial diagnostics: Isolation method and practical use.
  • Amálka Banni: Tumour cell induced platelet aggregation and platelet bioenergetics.

Impressions from IOC162


Walk and Talk:


Hotel Körbersee, Schröcken, AT
Information on travel and venue: IOC Schroecken.
» As there is no ATM close to the hotel, we recommend withdrawing money at Bregenz train station. Please make sure to bring some cash in EUROs with you, in case you need it.
The hotel accepts payment by credit card for consumed drinks.
» Please note that the hotel has a limited number of rooms and space is not guaranteed. Therefore, we ask that you to confirm with us in advance if you intend to bring accompanying guests with you.

Impressions from Hotel Koerbersee:


Oroboros Instruments
High-Resolution Respirometry
Schoepfstrasse 18
A-6020 Innsbruck, Austria
Tel: +43 677 647 929 17
Fax: +43 512 566796 20
Mitochondria and Cell Research

IOC recommended reading

The Must-Read for the O2k-Workshop:
Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. - »Bioblast link« - A new edition with updated terminology and more ..
» Chapter 2: Respiratory states and rates (estimated reading time 30 min – 1 h)
» Chapters 4-5: NADH-linked pathways through Complex CI and S-pathway through Complex CII (estimated reading time 30 min – 1 h)
» Chapter 6: Convergent electron transfer at the Q-junction (estimated reading time 45 min – 1 h 30 min)
Further reading:
» IOC recommended reading
» MitoPedia: Respiratory states

O2k-Workshops are listed as MitoGlobal Events.


ORO, IOC, MitoFit, 2023, MitoGlobal, NextGen-O2k, O2k-Network Award Previous 

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