MitoPediaAlert2020 Archive
- Currently Answered Questions from the Β»Oroboros Ecosystem AgendaΒ«
May 2020
- May-07 Th
Do you know how to evaluate fatty acid oxidation (FAO, F-pathway) avoiding to overestimate it? Did you know that malate alone might support mt-respiration in mitochondrial preparations, if malate anaplerotic pathways (e.g. mt-malic enzyme) are present?
- Learn more about - Β»malate anaplerotic pathway control stateΒ«
- DatLab 7.4 provides a step-by-step guide through the SUIT-027 to evaluate the malate anaplerotic pathway - Β»SUIT-027Β«
- Since F-pathway can be overestimated due to anaplerotic reactions, SUIT-002 was specially designed to give information on F-pathway in OXPHOS state avoiding FAO overestimation in the presence of anaplerotic pathways - Β»SUIT-002Β«
Apr 2020
- Apr-24 Fr
ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the electron transfer system.
- Apr-21 Tu
Citrate synthase activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.
- Apr-13 Mo
Is your mitochondrial preparation contaminated with other membranous organelles containing ATPases?
DatLab 7.4 provides a step-by-step guide through the SUIT-024 as a quality control protocol of the mitochondrial preparation
- Β»SUIT-024Β«
- Apr-08 We
The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage.
DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum digitonin concentration.
- Β»SUIT-010Β«
- Apr-02 Th
Do you want to search the best SUIT protocol for your research questions?
- Use the SUITbrowser: Β»SUITbrowserΒ«
Mar 2020
- Mar-16 Mo
The standardized coupling control protocol to explore the bioenergetics of living cells - DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions. - Β»SUIT-003Β«
- Mar-11 We
Does blebbistatin reduce oxygen dependence of respiration of permeabilized muscle fibers? This is one of the questions addressed in the COST Action CA15203 MitoEAGLE WG2. - Β»BlebbistatinΒ«
- Mar-06 Fr
Explore metabolic profiles and specific modulations of mitochondrial pathways in health and disease with SUIT reference protocols.
- Mar-03 Tu
Rapamycin - more differences or more in common with other mitObesity drugs? - Β»RapamycinΒ«
Feb 2020
- Feb-26 We
It is essential to titrate uncouplers in multiple steps for respirometric measurement of electron transfer capacity. - Β»Noncoupled respirationΒ«
- Feb-24 Mo
- Feb-19 We
Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function. - Β»SpermidineΒ«
- Feb-18 Tu
Why do we fight gas bubbles? - Dive deep into Β»Oxygen solubilityΒ«
- Feb-12 We
What do metformin, melatonin and other bioactive mitObesity compounds have in common? - Β»MitObesity drugsΒ«
Jan 2020
- Jan-16 Th
Oxygen flux - which SI units should be used?
Did you ever think that units for body mass could be an issue? - Β»Body massΒ«
- Jan-08 We
Force and Energy: this we all need in the New Year - and MitoPedia may help to clarify the difference.