Difference between revisions of "O2k-Catalogue: O2k-Modules"

Latest revision as of 17:10, 15 November 2019

O2k-Catalogue: O2k-Modules

Description	4#### O2k-Catalogue: O2k-Modules. O2k-Modules can be obtained with the O2k or added later, and can be simply installed by the user.
Product ID	4####
Type	O2k, MultiSensor
Link
Image	4####

O2k-Modules

O2k-Modules are add-on modules supported by the O2k. When one (or more) analytical parameters are monitored simultaneously with oxygen concentration and oxygen flux in the O2k, this is an O2k-MultiSensor application. The O2k fully supports the O2k-Modules.

High-resolution respirometry is extended by O2k-MultiSensor modules allowing the simultaneous measurement of oxygen consumption and one additional parameter. Fluorometric methods are available for a wide range of analytical parameters of major interest in mitochochondrial physiology: H₂O₂ production, mitochondrial membrane potential, intracellular pH, Ca²⁺, Mg²⁺, and NADH levels.

Advantages of the simultaneous measurement of additional parameters in a single respirometric chamber:

Quality control of preparations by respirometric performance.
Objective exclusion criteria for protocol related problems, e.g. overtitration of uncouplers, side effects of TPP⁺ or fluorophores.
Elimination of artifacts of normalization for a mitochondrial marker when combining two methods in a single chamber.
Additional information is acquired for a limited amount of sample.
Oxygen dependence of parameters can be studied.

O2k-Catalogue: O2k-Modules

O2k-MultiSensor Manual

Chapter	Section	Last update
MiPNet15.03 O2k-MultiSensor-ISE	O2k-MultiSensor system with ion selective electrodes (ISE).	2021-08-02
MiPNet15.05 NO-manual	O2k-MultiSensor system with amperometric sensors: NO, H₂S, H₂O₂.	2016-04-21
MiPNet17.05 O2k-Fluo LED2-Module	O2k-Fluo LED2-Module.	2021-06-22
MiPNet23.15 O2k-pH ISE-Module	Measurement of proton flux with the O2k-pH ISE-Module.	2018-09-08
MiPNet24.12 NextGen-O2k: Q-Module	NextGen-O2k: Q-Module manual	2021-10-29
MiPNet26.11 NextGen-O2k: PB-Module	NextGen-O2k: PB-Module manual	2021-10-29
MiPNet26.12 NextGen-O2k: NADH-Module	NextGen-O2k: NADH-Module manual	2022-05-17
MiPNet28.09 O2k-Fluo Smart-Module manual	O2k-Fluo Smart-Module manual	2023-09-25

»O2k-Publications: O2k-Fluorometry

References

Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38. - »Bioblast link«

Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. - »Bioblast link«

@@ Line 1: / Line 1: @@
-{{OroboroPedia}}
+{{Product
+|description='''4'''#### '''''O2k-Catalogue: O2k-Modules'''''. O2k-Modules can be obtained with the O2k or added later, and can be simply installed by the user.
-== '''List of O2k-MultiSensor products''' ==
+|product id=4'''####'''
+|product type=O2k, MultiSensor,
+|product image='''4'''####
+}}
-Please visit the [http://www.oroboros.at/index.php?id=mipnetanalyzer MultiSensor page] for an overview of MultiSensor options for the O2k.
+[[File:O2k-Core to FluoRespirometer.jpg|right|400px |O2k-Core to FluoRespirometer]]
+== O2k-Modules ==
-'''Back to: [[List of all products]]'''
+'''O2k-Modules''' are add-on modules supported by the O2k. When one (or more) analytical parameters are monitored simultaneously with oxygen concentration and oxygen flux in the O2k, this is an '''O2k-MultiSensor''' application.  The O2k fully supports the O2k-Modules.
+* [[MiPNet17.05 O2k-Fluo LED2-Module]]
+* [[O2k-TPP%2B_ISE-Module]]
+* [[O2k-pH ISE-Module]]
+* [[O2k-NO Amp-Module]]
+* [[Hydrogen peroxide]]
+* [[Cytochrome Spectra and Oxygen]]
+[[High-resolution respirometry]] is extended by O2k-MultiSensor modules allowing the simultaneous measurement of oxygen consumption and one additional parameter. Fluorometric methods are available for a wide range of analytical parameters of major interest in mitochochondrial physiology: [[O2k-Publications:_O2k-Fluorometry_-_Fluorophores|H<sub>2</sub>O<sub>2</sub> production, mitochondrial membrane potential, intracellular pH, Ca<sup>2+</sup>, Mg<sup>2+</sup>, and NADH levels]].
-Submit your order: [[Has mailaddress::{{{mailaddress|instruments@oroboros.at}}}]]
+Advantages of the simultaneous measurement of additional parameters in a single respirometric chamber:
+# Quality control of preparations by respirometric performance.
+# Objective exclusion criteria for protocol related problems, e.g. overtitration of uncouplers, side effects of TPP<sup>+</sup> or fluorophores.
+# Elimination of artifacts of normalization for a mitochondrial marker when combining two methods in a single chamber.
+# Additional information is acquired for a limited amount of sample.
+# Oxygen dependence of parameters can be studied.
-{{#ask: mainlabel=Title|[[Category:Products]] | [[Product type::MultiSensor]]
+== '''O2k-Catalogue: O2k-Modules''' ==
+{{#ask:[[Category:Products]][[Product type::O2k-Module]]
 | ?description
 | ?product id
 | ?product image
+|sort=product id
+|order=ascending
+}}
+== O2k-MultiSensor Manual ==
+{{#ask:[[Category:Publications]] [[Instrument and method::O2k-Manual]] [[Additional label::O2k-MultiSensor]]
+| mainlabel=Chapter
+|?Has title=Section
+|?Was published in year=Last update
+|format=broadtable
+|limit=500
+|sort=
+|order=ascending
+|offset=0
 }}
+: »[[O2k-Publications: O2k-Fluorometry]]
+== References ==
+:::* Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38. - [[Makrecka-Kuka 2015 Biomolecules |»Bioblast link«]]
+:::* Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. - [[Krumschnabel 2015 Methods Mol Biol |»Bioblast link«]]