Difference between revisions of "IOC recommended reading"
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<big>Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp. - [[Gnaiger 2014 MitoPathways |Ā»Bioblast linkĀ«]]</big> | <big>Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp. - [[Gnaiger 2014 MitoPathways |Ā»Bioblast linkĀ«]]</big> | ||
:* ''A hard copy of this monograph on mitochondrial physiology will be included in the workshop material. The Blue Book is available on-line free of charge and provides a background on OXPHOS analysis'': | ::::* ''A hard copy of this monograph on mitochondrial physiology will be included in the workshop material. The Blue Book is available on-line free of charge and provides a background on OXPHOS analysis'': | ||
:* From oxygen concentration to oxygen flux '''ā¢ā¢''' Membrane potential: electric and chemical forces '''ā¢ā¢''' Coupling and efficiency '''ā¢ā¢''' Biochemical thresholds | ::::* From oxygen concentration to oxygen flux '''ā¢ā¢''' Membrane potential: electric and chemical forces '''ā¢ā¢''' Coupling and efficiency '''ā¢ā¢''' Biochemical thresholds | ||
:* Respiratory states | ::::* Respiratory states | ||
:* MitoPathways to electron transfer complexes | ::::* MitoPathways to electron transfer complexes | ||
:* Normalization of mitochondrial respiration: coupling control and substrate control. | ::::* Normalization of mitochondrial respiration: coupling control and substrate control. | ||
<big>Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. - [[Gnaiger_2008_POS |Ā»Bioblast linkĀ«]]</big> | <big>Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. - [[Gnaiger_2008_POS |Ā»Bioblast linkĀ«]]</big> | ||
:* Methodological introduction, comparison of HRR with traditional oxygraphy. | ::::* Methodological introduction, comparison of HRR with traditional oxygraphy. | ||
:* Dramatically different: high-resolution respirometry - the OROBOROS [[Oxygraph-2k]]. | ::::* Dramatically different: high-resolution respirometry - the OROBOROS [[Oxygraph-2k]]. | ||
:* Calibration of polarographic oxygen sensors and oxygen concentration in respiration media at air saturation. | ::::* Calibration of polarographic oxygen sensors and oxygen concentration in respiration media at air saturation. | ||
:* From Oxygraph slopes to respiratory flux corrected for background effects. | ::::* From Oxygraph slopes to respiratory flux corrected for background effects. | ||
:* Phosphorylation or coupling control protocol (PCP, CCP) with intact cells. | ::::* Phosphorylation or coupling control protocol (PCP, CCP) with intact cells. | ||
:* Titration steps of the CCP. | ::::* Titration steps of the CCP. | ||
:* Experimental example. | ::::* Experimental example. | ||
:* Flux control ratios from the CCP. | ::::* Flux control ratios from the CCP. | ||
:* Intact cells, permeabilized cells and tissue, or isolated mitochondria? | ::::* Intact cells, permeabilized cells and tissue, or isolated mitochondria? | ||
<big>Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128:277-97. - [[Gnaiger 2001 Respir Physiol |Ā»Bioblast linkĀ«]]</big> Ā | <big>Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128:277-97. - [[Gnaiger 2001 Respir Physiol |Ā»Bioblast linkĀ«]]</big> Ā | ||
:* A detailed introduction into high-resolution respirometry with particular emphasis on kinetics and measurements at low oxygen | ::::* A detailed introduction into high-resolution respirometry with particular emphasis on kinetics and measurements at low oxygen | ||
:* Mitochondrial kinetics measured by high-resolution respirometry Ā | ::::* Mitochondrial kinetics measured by high-resolution respirometry Ā | ||
:* Calibrations and corrections for response time and instrumental background Ā | ::::* Calibrations and corrections for response time and instrumental background Ā | ||
:* Steady-state injection respirometry Ā | ::::* Steady-state injection respirometry Ā | ||
:* Mitochondrial respiratory control at low oxygen Ā | ::::* Mitochondrial respiratory control at low oxygen Ā | ||
:* Apparent oxygen affinity and catalytic efficiency of mitochondrial respiration Ā | ::::* Apparent oxygen affinity and catalytic efficiency of mitochondrial respiration Ā | ||
:* Effect of ADP and oxygen limitation on ADP/O2 flux ratios Ā | ::::* Effect of ADP and oxygen limitation on ADP/O2 flux ratios Ā | ||
:* The low-oxygen environment of the cell: Mitochondria between hypoxic and oxidative stress | ::::* The low-oxygen environment of the cell: Mitochondria between hypoxic and oxidative stress | ||
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Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp. - [[Gnaiger 2014 MitoPathways |Ā»Bioblast linkĀ«]] | Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp. - [[Gnaiger 2014 MitoPathways |Ā»Bioblast linkĀ«]] | ||
:* The Blue Book: OXPHOS analysis; CI-linked, CII-linked, CI<small>&</small>II-linked pathways, respiratory states, abbreviations. | ::::* The Blue Book: OXPHOS analysis; CI-linked, CII-linked, CI<small>&</small>II-linked pathways, respiratory states, abbreviations. | ||
Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58. - [[Pesta 2012 Methods Mol Biol |Ā»Bioblast linkĀ«]] | Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58. - [[Pesta 2012 Methods Mol Biol |Ā»Bioblast linkĀ«]] | ||
:* Introduction to high-resolution respirometry (HRR), SUIT protocols and flux control ratios. | ::::* Introduction to high-resolution respirometry (HRR), SUIT protocols and flux control ratios. | ||
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== O2k-Fluorometry == | == O2k-Fluorometry == | ||
* '''O2k-Manual''': [[MiPNet17.05 O2k-Fluo LED2-Module|Ā»O2k-Fluo LED2-ModuleĀ«]] | ::::* '''O2k-Manual''': [[MiPNet17.05 O2k-Fluo LED2-Module|Ā»O2k-Fluo LED2-ModuleĀ«]] | ||
* Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolutionĀ respirometry for simultaneous measurement of oxygen and hydrogenĀ peroxide fluxes in permeabilized cells, tissue homogenate and isolatedĀ mitochondria. Biomolecules 5:1319-38. [[Makrecka-Kuka 2015 Biomolecules|Ā»Bioblast pdfĀ«]] | ::::* Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolutionĀ respirometry for simultaneous measurement of oxygen and hydrogenĀ peroxide fluxes in permeabilized cells, tissue homogenate and isolatedĀ mitochondria. Biomolecules 5:1319-38. [[Makrecka-Kuka 2015 Biomolecules|Ā»Bioblast pdfĀ«]] | ||
* Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. - [[Krumschnabel 2014 Methods Enzymol |Ā»Bioblast linkĀ«]] | Ā | ||
* Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. - [[Krumschnabel 2015 Methods Mol Biol |Ā»Bioblast linkĀ«]] | ::::* Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. - [[Krumschnabel 2014 Methods Enzymol |Ā»Bioblast linkĀ«]] | ||
* Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01):1-6. - [[MiPNet18.05 Amplex-Mouse-heart |Ā»Bioblast linkĀ«]] | Ā | ||
* '''[[O2k-Publications: O2k-Fluorometry]]''' | ::::* Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. - [[Krumschnabel 2015 Methods Mol Biol |Ā»Bioblast linkĀ«]] | ||
Ā | |||
::::* Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01):1-6. - [[MiPNet18.05 Amplex-Mouse-heart |Ā»Bioblast linkĀ«]] | |||
Ā | |||
::::* '''[[O2k-Publications: O2k-Fluorometry]]''' | |||
== TPP/ISE == | == TPP/ISE == | ||
* O2k-MultiSensor System with ion selective electrodes (ISE) '''[[MiPNet15.03 O2k-MultiSensor-ISE |MiPNet15.03]]''' | ::::* O2k-MultiSensor System with ion selective electrodes (ISE) '''[[MiPNet15.03 O2k-MultiSensor-ISE |MiPNet15.03]]''' | ||
* Determination of membrane potential using TPP+ and an ion selective electrode (ISE) '''[[MiPNet14.05]]''' | Ā | ||
* Rottenberg H (1984) Membrane potential and surface potential in mitochondria: uptake and binding of lipophilic cations. J Membr Biol 81:127-38. | ::::* Determination of membrane potential using TPP+ and an ion selective electrode (ISE) '''[[MiPNet14.05]]''' | ||
Ā | |||
::::* Rottenberg H (1984) Membrane potential and surface potential in mitochondria: uptake and binding of lipophilic cations. J Membr Biol 81:127-38. | |||
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Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41: 1837-1845. - [[Gnaiger 2009 Int J Biochem Cell Biol |Ā»Bioblast linkĀ«]] | Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41: 1837-1845. - [[Gnaiger 2009 Int J Biochem Cell Biol |Ā»Bioblast linkĀ«]] | ||
:* Respirometry with permeabilized fibres and isolated mitochondria. | ::::* Respirometry with permeabilized fibres and isolated mitochondria. | ||
:* Convergent CI<small>&</small>II electron input and OXPHOS capacity. | ::::* Convergent NS (CI<small>&</small>II) electron input and OXPHOS capacity. | ||
:* Tissue-OXPHOS capacity in human permeabilized muscle fibres and isolated mitochondria. | ::::* Tissue-OXPHOS capacity in human permeabilized muscle fibres and isolated mitochondria. | ||
:* Tissue-OXPHOS capacity and functional diversity. | ::::* Tissue-OXPHOS capacity and functional diversity. | ||
Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: Facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662: 7-25. - [[Scandurra 2010 Adv Exp Med Biol |Ā»Bioblast linkĀ«]] | Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: Facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662: 7-25. - [[Scandurra 2010 Adv Exp Med Biol |Ā»Bioblast linkĀ«]] | ||
:* Isolated mitochondria and intact cells. Ā | ::::* Isolated mitochondria and intact cells. Ā | ||
:* Nanomolar resolution of oxygen concentration. Ā | ::::* Nanomolar resolution of oxygen concentration. Ā | ||
:* Effects of cell density and activity. Ā | ::::* Effects of cell density and activity. Ā | ||
:* Closed versus open systems. Ā | ::::* Closed versus open systems. Ā | ||
:* ''p''<sub>50</sub> in vivo. | ::::* ''p''<sub>50</sub> in vivo. | ||
Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000) Mitochondria in the cold. In: Life in the Cold (Heldmaier G, Klingenspor M, eds) Springer, Heidelberg, Berlin, New York:431-42. - [[Gnaiger 2000 Life in the Cold |Ā»Bioblast linkĀ«]] | Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000) Mitochondria in the cold. In: Life in the Cold (Heldmaier G, Klingenspor M, eds) Springer, Heidelberg, Berlin, New York:431-42. - [[Gnaiger 2000 Life in the Cold |Ā»Bioblast linkĀ«]] | ||
:* Isolated mitochondria and permeabilized muscle fibers. | ::::* Isolated mitochondria and permeabilized muscle fibers. | ||
:* Optimization of mitochondrial cold storage ā¢ Mitochondrial respiration medium, [[MiR05]] ā¢ Mitochondrial cold ischemia-reperfusion injury. | ::::* Optimization of mitochondrial cold storage ā¢ Mitochondrial respiration medium, [[MiR05]] ā¢ Mitochondrial cold ischemia-reperfusion injury. | ||
Renner K, Amberger A, Konwalinka G, Kofler R, Gnaiger E (2003) Changes of mitochondrial respiration, mitochondrial content and cell size after induction of apoptosis in leukemia cells. Biochim Biophys Acta 1642:115-23. - [[Renner 2003 Biochim Biophys Acta |Ā»Bioblast linkĀ«]] Ā | Renner K, Amberger A, Konwalinka G, Kofler R, Gnaiger E (2003) Changes of mitochondrial respiration, mitochondrial content and cell size after induction of apoptosis in leukemia cells. Biochim Biophys Acta 1642:115-23. - [[Renner 2003 Biochim Biophys Acta |Ā»Bioblast linkĀ«]] Ā | ||
:* Intact cells. Ā | ::::* Intact cells. Ā | ||
:* Cytochrome ''c'' oxidase. | ::::* Cytochrome ''c'' oxidase. | ||
:* Cytochrome ''c'' test. | ::::* Cytochrome ''c'' test. | ||
:* Respiration per million cells, per citrate synthase, per mg protein, or per cytochrome ''c'' oxidase activity. | ::::* Respiration per million cells, per citrate synthase, per mg protein, or per cytochrome ''c'' oxidase activity. | ||
Gnaiger E, Steinlechner-Maran R, MĆ©ndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27:583-96. - [[Gnaiger 1995 J Bioenerg Biomembr |Ā»Bioblast linkĀ«]] | Gnaiger E, Steinlechner-Maran R, MĆ©ndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27:583-96. - [[Gnaiger 1995 J Bioenerg Biomembr |Ā»Bioblast linkĀ«]] | ||
:* First publication to establish high-resolution respirometry, based on the OROBOROS Oxygraph. | ::::* First publication to establish high-resolution respirometry, based on the OROBOROS Oxygraph. | ||
:* Standardized oxygen calibration (air and internal zero oxygen calibration). | ::::* Standardized oxygen calibration (air and internal zero oxygen calibration). | ||
:* Automatic instrumental background correction of oxygen flux. | ::::* Automatic instrumental background correction of oxygen flux. | ||
:* Deconvolution of the oxygen signal for time resolution required in oxygen kinetics. | ::::* Deconvolution of the oxygen signal for time resolution required in oxygen kinetics. | ||
:* Quality control of the limit of detection of oxygen flux at Ā±1 pmol O<sub>2</sub>Ā·s<sup>-1</sup>Ā·ml<sup>-1</sup>. Ā | ::::* Quality control of the limit of detection of oxygen flux at Ā±1 pmol O<sub>2</sub>Ā·s<sup>-1</sup>Ā·ml<sup>-1</sup>. Ā | ||
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Ā» [[Media:MiPNet19.18 O2k-Core Manual.pdf|'''O2k-Core Manual.pdf''']]: O2k-Manual for [[DatLab 6]]. | ::::Ā» [[Media:MiPNet19.18 O2k-Core Manual.pdf|'''O2k-Core Manual.pdf''']]: O2k-Manual for [[DatLab 6]]. | ||
Ā» [[MiPNet17.09 IOC Questions | '''Worksheet O2k-Basic''']]: This worksheet contains questions on the O2k and HRR addressed to participants of O2k-Basic Workshops. Answers to these questions are elaborated during the O2k-Workshop. | ::::Ā» [[MiPNet17.09 IOC Questions | '''Worksheet O2k-Basic''']]: This worksheet contains questions on the O2k and HRR addressed to participants of O2k-Basic Workshops. Answers to these questions are elaborated during the O2k-Workshop. | ||
Ā» [[O2k-Publications|'''O2k-Publications''']] | ::::Ā» [[O2k-Publications|'''O2k-Publications''']] | ||
Ā» [[O2k-Protocols|'''O2k-Protocols''']] | ::::Ā» [[O2k-Protocols|'''O2k-Protocols''']] | ||
[[Category:OroboPedia]] | [[Category:OroboPedia]] |
Revision as of 08:25, 3 March 2016
IOC recommended reading
HRR: high-resolution respirometry
Recommended reading
Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp. - Ā»Bioblast linkĀ«
- A hard copy of this monograph on mitochondrial physiology will be included in the workshop material. The Blue Book is available on-line free of charge and provides a background on OXPHOS analysis:
- From oxygen concentration to oxygen flux ā¢ā¢ Membrane potential: electric and chemical forces ā¢ā¢ Coupling and efficiency ā¢ā¢ Biochemical thresholds
- Respiratory states
- MitoPathways to electron transfer complexes
- Normalization of mitochondrial respiration: coupling control and substrate control.
Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. - Ā»Bioblast linkĀ«
- Methodological introduction, comparison of HRR with traditional oxygraphy.
- Dramatically different: high-resolution respirometry - the OROBOROS Oxygraph-2k.
- Calibration of polarographic oxygen sensors and oxygen concentration in respiration media at air saturation.
- From Oxygraph slopes to respiratory flux corrected for background effects.
- Phosphorylation or coupling control protocol (PCP, CCP) with intact cells.
- Titration steps of the CCP.
- Experimental example.
- Flux control ratios from the CCP.
- Intact cells, permeabilized cells and tissue, or isolated mitochondria?
Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128:277-97. - Ā»Bioblast linkĀ«
- A detailed introduction into high-resolution respirometry with particular emphasis on kinetics and measurements at low oxygen
- Mitochondrial kinetics measured by high-resolution respirometry
- Calibrations and corrections for response time and instrumental background
- Steady-state injection respirometry
- Mitochondrial respiratory control at low oxygen
- Apparent oxygen affinity and catalytic efficiency of mitochondrial respiration
- Effect of ADP and oxygen limitation on ADP/O2 flux ratios
- The low-oxygen environment of the cell: Mitochondria between hypoxic and oxidative stress
SUIT protocols for high-resolution respirometry
Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp. - Ā»Bioblast linkĀ«
- The Blue Book: OXPHOS analysis; CI-linked, CII-linked, CI&II-linked pathways, respiratory states, abbreviations.
Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58. - Ā»Bioblast linkĀ«
- Introduction to high-resolution respirometry (HRR), SUIT protocols and flux control ratios.
Ā» MitoPedia: Respiratory states
O2k-Fluorometry
- O2k-Manual: Ā»O2k-Fluo LED2-ModuleĀ«
- Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38. Ā»Bioblast pdfĀ«
- Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. - Ā»Bioblast linkĀ«
- Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. - Ā»Bioblast linkĀ«
- Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01):1-6. - Ā»Bioblast linkĀ«
TPP/ISE
- O2k-MultiSensor System with ion selective electrodes (ISE) MiPNet15.03
- Determination of membrane potential using TPP+ and an ion selective electrode (ISE) MiPNet14.05
- Rottenberg H (1984) Membrane potential and surface potential in mitochondria: uptake and binding of lipophilic cations. J Membr Biol 81:127-38.
Further reading
Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41: 1837-1845. - Ā»Bioblast linkĀ«
- Respirometry with permeabilized fibres and isolated mitochondria.
- Convergent NS (CI&II) electron input and OXPHOS capacity.
- Tissue-OXPHOS capacity in human permeabilized muscle fibres and isolated mitochondria.
- Tissue-OXPHOS capacity and functional diversity.
Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: Facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662: 7-25. - Ā»Bioblast linkĀ«
- Isolated mitochondria and intact cells.
- Nanomolar resolution of oxygen concentration.
- Effects of cell density and activity.
- Closed versus open systems.
- p50 in vivo.
Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000) Mitochondria in the cold. In: Life in the Cold (Heldmaier G, Klingenspor M, eds) Springer, Heidelberg, Berlin, New York:431-42. - Ā»Bioblast linkĀ«
- Isolated mitochondria and permeabilized muscle fibers.
- Optimization of mitochondrial cold storage ā¢ Mitochondrial respiration medium, MiR05 ā¢ Mitochondrial cold ischemia-reperfusion injury.
Renner K, Amberger A, Konwalinka G, Kofler R, Gnaiger E (2003) Changes of mitochondrial respiration, mitochondrial content and cell size after induction of apoptosis in leukemia cells. Biochim Biophys Acta 1642:115-23. - Ā»Bioblast linkĀ«
- Intact cells.
- Cytochrome c oxidase.
- Cytochrome c test.
- Respiration per million cells, per citrate synthase, per mg protein, or per cytochrome c oxidase activity.
Gnaiger E, Steinlechner-Maran R, MĆ©ndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27:583-96. - Ā»Bioblast linkĀ«
- First publication to establish high-resolution respirometry, based on the OROBOROS Oxygraph.
- Standardized oxygen calibration (air and internal zero oxygen calibration).
- Automatic instrumental background correction of oxygen flux.
- Deconvolution of the oxygen signal for time resolution required in oxygen kinetics.
- Quality control of the limit of detection of oxygen flux at Ā±1 pmol O2Ā·s-1Ā·ml-1.
General
- Ā» O2k-Core Manual.pdf: O2k-Manual for DatLab 6.
- Ā» Worksheet O2k-Basic: This worksheet contains questions on the O2k and HRR addressed to participants of O2k-Basic Workshops. Answers to these questions are elaborated during the O2k-Workshop.