MiPNet23.14 IOC135 Busan KR

                

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MiPNet23.14 IOC135 Busan KR

ASMRM 2018, Busan KR, 2018 Nov 06 to 07. Oroboros for MIRACLE - the bioenergetic snapshot of mitochondrial fitness (IOC135).

» ASMRM 2018 Busan KR

Oroboros (2018-11-06) Mitochondr Physiol Network

Abstract:

» ASMRM 2018 Busan KR

• O2k-Network Lab: AT Innsbruck Oroboros

O2k-Workshop

Basic and advanced applications of the Oroboros O2k-FluoRespirometer

Time

Nov 6, 14:00-20:00

Location

National Research Laboratory for Mitochondrial Signaling

Department of Physiology

Inje College of Medicine

Cardiovascular and Metabolic Disease Center

Inje University

Gaegume 2 dong, Busan, South Korea

Address

3rd Floor, Experimental Room

Bokjiro 75, Busan Jin Gu, Busan City, Korea

https://goo.gl/maps/JATb11LcY5N2

Local organizers

Prof. Dr. Jin Han

Associate Prof. Dr. Hyoung Kyu Kim

username: @mitophysiol (telegram)

https://t.me/mitophysiol

Phone: +82-51-890-8778

Cardiovascular and Metabolic Disease Center (CMDC)

Inje University

Gaegume 2 dong, Busan, Korea

KR Busan Han J - Oroboros O2k-Training Centre

Programme - with O2k demo experiment

14:00 Welcome

14:15 Recent instrumental innovations

• O2k-FluoRespirometer - the concept
• Smart Fluo-Sensors (O2k-Series H)
• O2k-Titration Set

Protocol menu

14:45 Start O2k demo experiment - SUIT-9 AmR pce D19 (O₂ and H₂O₂ flux with permeabilized cells)

15:00 Innovations in DatLab 7 - and more in MitoPedia: DatLab

• DatLab 7 and instrumental quality control: SOPs from cleaning to instrumental background test
• SUIT-Protocols » Run DL-Protocol/Set O2 limit
• Correction and normalization of flux » Flux / Slope
• New features of Mark statistics

16:00 Break

16:30 Bioenergetic snapshot of mitochondrial fitness: Select the appropriate SUIT protocol

18:00 DatLab Analysis: Evaluation of the O2k demo experiment

18:30 DatLab Analysis: Flux per volume, flux per mass, flow per cell, flux control ratio, flux control factor

19:45 Conclusions

Contact

Hyoung Kyu Kim. PhD.

Associate professor

Username: @mitophysiol (telegram)

https://t.me/mitophysiol

Phone: +82-51-890-8778

인제대학교 심혈관대사질환센터 부교수

김형규 올림

IOC recommended reading

» IOC recommended reading

ASMRM seminar by Oroboros

Nov 7 (Wed) 10:30-11:30, Park Hyatt Busan, Busan, Korea

Lecture: Oroboros for MIRACLE - the bioenergetic snapshot of mitochondrial fitness

Erich Gnaiger, PhD., Oroboros Instruments, CEO

Oroboros Instruments is a member of COST Action CA15203 MitoEAGLE

Mitochondrial research and medicine at centre stage

The Oroboros O2k-FluoRespirometer provides the flexibility required to meet current demands in mitochondrial research and medicine:

• Isolated mitochondria
• Tissue homogenates
• Permeabilized tissues
• Permeabilized cells
• Intac cells
• Enzyme preparations

The bioenergetic snapshot of mitochondrial fitness

• Fight the reproducibility crisis: shoot with accuracy
• Analyze mitochondrial complexity: snapshot of mt-pathway and coupling control
• Combine state and rate analysis:

• O₂ flux, O₂ concentration (hypoxia and hyperoxia)
• mitochondrial membrane potential
• ATP production
• ROS production
• Ca²⁺

The largest global mitochondrial network

• 3,000 O2k-Publications
• Join the O2k-Network
• Benefit from the unique features of DatLab 7 with SUIT protocols
• World-wide innovation in mitochondrial respirometry: interlaboratory MitoFit Proficiency Test

Oroboros exhibition

Oroboros and KR Seoul Scitech Korea are looking forward to welcoming you at the booth of Scitech Korea at ASMRM 2018.

• 

  Erich Gnaiger, PhD., Oroboros Instruments, CEO

• 

  Andrea Gnaiger, Mag., Oroboros Instruments, CFO

Labels:

ORO, IOC, MitoGlobal, 2018, Next