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|title=Stock D, Leslie AGW, Walker JE (1999) Molecular architecture of the rotary motor in |authors=Stock D, Leslie AGW, Walker JE

|description='''2-Deoxyglucose''', also known as 2-deoxy-D-glucose is a glucose derivative that has the 2-hydroxyl group replaced by h :::'''2-DG: 2-deoxy-D-glucose''' (C₆H₁₂0₅); Sigma D6134, 1 g

...tor). High concentrations of antimycin A also inhibit acyl-CoA oxidase and D-amino acid oxidase. :::: '''Preparation of 5 mM stock solution''' (dissolved in ethanol abs.) for '''2-mL O2k-chamber''':

|description='''Glucose''', also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in b |complete name=D-(+)-Glucose

...raction is not well understood. For example, the deficiency of cyclophilin D in KO models does not prevent mitochondria from permeability transition and ...1</sub>N₁₁O₁₂); Sigma 30024, 25 mg, store commercial stock at 2-8°C; M = 1202.61 g·mol^-1 (≥ 95%).-->

...: '''Dig: Digitonin''' (C₅₆H₉₂O₂₉); Sigma D 5628, 1 g, store at RT; M = 1229.31 g·mol^-1--> ::::'''Preparation of 10 mg/mL (8.1 mM) stock solution (dissolved in DMSO)''' for '''2-mL O2k-chamber''':

...s may be required because of a report of the structure of ATP synthase (D. Stock, A.G.W. Leslie, J.E. Walker, Science 286 (1999) 1700-1705) which suggests t

::::'''Preparation of 1 mM storage and stock solution''' (dissolved in ethanol abs.) for '''2-mL O2k-chamber''': ::::'''Preparation of 0.25 mM stock solution''' (dissolved in ethanol abs.) for '''0.5-mL O2k-chamber''':

...itochondrial matrix for subsequent β-oxidation. There are two enantiomers: D- and L-carnitine. Only the L-isomer is physiologically active. :::: '''Preparation of 250 mM stock solution''' (dissolved in H₂O)

|abbr=D |abbr=D

:::: '''Preparation of 1 M stock solution''' (1 mL, dissolved in H₂O): :::: '''Preparation of 1 M stock solution''' (dissolved in H₂O):

:::: '''Preparation of 500 mM ATP stock solution''' :::: ''' Preparation of 500 mM ATP stock solution with 400 mM free Mg²⁺ '''

...esdorf A, Casero D, Ent P, Castruita M, Pellegrini M, Merchant SS, Malasam D (2011) A revised mineral nutrient supplement increases biomass and growth r ...medium. Other advantages include more facile preparation of trace element stock solutions, a reduction in total chemical use, a more consistent batch-to-ba

Communicated by [[Cardoso LHD]], [[Antunes D]] and [[Gnaiger E]] (last update 2019-07-31) ::: Suggested stock concentrations are shown in the specific DL-Protocol.

{{Template:SUIT-017 O2 mt D}} ::: Suggested stock concentrations are shown in the specific DL-Protocol.

...therefore near identical optical properties. They differ in their K_D values and are suitable for measuring different ranges of calcium co ::::* Calcium Green 1: K_D = 190 nM

::: '''Preparation of 1.1 mM stock solution''' (dissolved in H₂O): :::: Titration into 2 mL O2k-Chamber: 2 µL of 1.1 mM MgG stock solution, final concentration of 1.1 µM MgG.

Communicated by [[Komlodi T]], [[Antunes D]], [[Garcia-Souza LF]], [[Cardoso LH]], [[Gnaiger E]] (last update 2019-07- ::: Suggested stock concentrations are shown in the specific DL-Protocol.

...a calibration with the fluorescent dye and the determination of the K_d of ADP and ATP to Mg²⁺ in the pertaining experimental cond :::* [[Magnesium_Green#Preparation_of_other_solutions|ADP]] (D)

...a calibration with the fluorescent dye and the determination of the K_d of ADP and ATP to Mg²⁺ in the pertaining experimental cond :::* [[Magnesium_Green#Preparation_of_other_solutions|ADP]] (D)

...mol] || SI; amount ''n''_''X'' of ''X'' and change of the amount d''n''_''X'' of ''X''; Eq. 8.3 | mass, dry mass || [[Body mass]] || ''m''_d ||[kg] || Fig. 5 (dry weight)

...discharged '''(3)'''. The same or larger volume ''V''_J.''i'' of stock cell suspension J is pipetted into the chamber '''(4)'''. The stirrer may b ...er further dilution from s to stock J. Subsamples are transferred from the stock cell suspension J into the O2k-chamber in a variety of ways, selection of w

...chondria leads to the hyperpolarisation of the mt-membrane, while [[ADP]] (D) decreases the mt-membrane potential. Addition of [[oligomycin]] (Omy) resu ::: Suggested stock concentrations are shown in the specific DL-Protocol.

Communicated by [[Huete-Ortega M]], [[Antunes D]], [[Komlodi T]] and [[Gnaiger E]] (last update 2019-07-25) ::: Suggested stock concentrations are shown in the specific DL-Protocol.

...n narrower slices of previous work 18,46, thereby limiting the ‘effective’ stock of knowledge. ::::::* First, we see a decline in the diversity of work cited (Fig. 6a,d), indicating that contemporary science and technology are engaging with nar

...the mitochondria leads to hyperpolarisation of the mt-membrane, while ADP (D) decreases the mt-membrane potential. Succinate (S) is able to further incr ::: Suggested stock concentrations are shown in the specific DL-Protocol.

|abstract='''Pesta D, Gnaiger E (2015) Preparation of permeabilized muscle fibers for diagnosis :::* [[Pesta_2012_Methods_Mol_Biol| Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human

|| stock || stock

:::: '''Preparation of 10 mM stock solution- Conditioning solution 1''' (dissolved in 100 mM KCl solution) fo :::: '''Preparation of 1 µM stock solution- Conditioning solution 2''' (dissolved in 100 mM KCl solution) fo

:::## Fill with CCCP stock solution and start the experiment. ...k. From E-0032 onward all O2ks have an upgrade for the TIP2k. O2k series A-D can be upgraded for TIP2k for an extra fee.

...W, Leuner K, Müller WE, Kudin AP, Kunz WS, Zimmermann A, Roeper J, Wenzel D, Jendrach M, García-Arencíbia M, Fernández-Ruiz J, Huber L, Rohrer H, Ba * Kamienieva I, Charzyńska A, Duszyński J, Malińska D, Szczepanowska J (2023) In search for mitochondrial biomarkers of Parkinson

::: '''Preparation of 1 mM stock solution''' ::::* [[ADP]] (D)

...he O2k-Network »[[US FL Orlando Goodpaster BH]]« and »[[IE Dublin O Gorman D]]« ...z EE, Akkermann D, Paul C, Schwagarus N, Müller S, Odle A, Childs G, Ewers D, Kungl T, Sitte M, Salinas G, Sereda MW, Nave KA, Schwab MH, Ost M, Arthur-

*Pham T, MacRae CL, Broome SC, D'souza RF, Narang R, Wang HW, Mori TA, Hickey AJR, Mitchell CJ, Merry TL (20 ...u Z, Li L, Zhang H, Sun F, Ma H, Wang L, Hu Y, Yan Z, Zheng H, Yang G, Liu D, Tepel M, Gao P, Zhu Z (2020) Reducing NADPH synthesis counteracts diabetic

:::# Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human :::# Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and ox

:::: Enhance the rigorous training activities particularly for Ph.D. students and young researchers (STSM, Schools, Workshops, Conferences) in | [[Kucera O]], [[Lotkova H]], [[Stankova P]], [[Endlicher R]], [[Rychtrmoc D]], [[Sobotka O]]