Template:SUIT-003 O2 ce D038

Steps and respiratory states

Step	State	Pathway	Q-junction	Comment - Events (E) and Marks (M)
ce1	ROUTINE			ce1 ROUTINE respiration in the physiological coupling state R. Externally added permeable substrates could contribute to this respiratory state.
ce2(Omy)	L			ce1;ce2(Omy) Non-phosphorylating resting state (LEAK state); LEAK-respiration, L(Omy), after blocking the ATP synthase with oligomycin.
ce3U	E			ce1;ce2(Omy);ce3U Noncoupled electron transfer state, ET state, with ET capacity E.
ce3Glc	E			ce1;ce2(Omy);ce3U;ce3Glc Noncoupled electron transfer state, ET state, with ET capacity E. Glucose is added to test Crabtree effect.
ce3(U)	E			ce1;ce2(Omy);ce3U;ce3Glc;ce3(U) Noncoupled electron transfer state, ET state, with ET capacity E. Additional uncoupler titrations are performed to ensure reaching ET state after addition of glucose.
ce4Ama	ROX			ce1;ce2(Omy);ce3U;ce3Glc;ce3(U);ce4Ama Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated either after inhibition of CIII (e.g. antimycin A, myxothiazol), CIV (e.g. Cyanide) or in the absence of endogenous fuel-substrates. Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration.

Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

Coupling control

» Coupling control state

» ET capacity

» OXPHOS capacity

» LEAK respiration

Pathway control

» Electron transfer pathway

» Fatty acid oxidation pathway control state, F

» NADH electron transfer-pathway state, N

» Succinate pathway control state, S

» NS-pathway control state, NS

» Glycerophosphate pathway control state, Gp

» Complex IV single step, CIV

» Anaplerotic pathway control state

Main fuel substrates

» Glutamate, G

» Glycerophosphate, Gp

» Malate, M

» Octanoylcarnitine, Oct

» Pyruvate, P

» Succinate, S

Glossary

» List of SUIT states

» SUIT concept