Difference between revisions of "Template:SUIT-003 ce1;ce2;ce3"

Revision as of 17:29, 25 January 2019

Steps and respiratory states

Step	State	Comment - Events (E) and Marks (M)
ce1	ROUTINE	ce1 ROUTINE respiration in the physiological coupling state R. Externally added permeable substrates could contribute to this respiratory state.
ce2(Omy)	L	ce1;ce2Omy Non-phosphorylating resting state (LEAK state); LEAK-respiration, L(Omy), after blocking the ATP synthase with oligomycin. The addition of oligomycin or its carrier (EtOH) is optional depending on the experimental question to resolve. If we add the carrier we do not reach LEAK state and we will evaluate if the EtOH alters the ROUTINE state.
ce3U	E	ce1;ce2Omy;ce3U Uncoupler titration (avoiding inhibition by high uncoupler concentrations) to obtain electron transfer (ET) capacity E (noncoupled ET-state). Test for limitation of OXPHOS capacity P by the phosphorylation system (ANT, ATP synthase, phosphate transporter) relative to ET capacity E in mt-preparations: E-P control efficiency and E-L coupling efficiency. In living cells: E-R control efficiency and E-L coupling efficiency. Noncoupled electron transfer state, ET state, with ET capacity E.
ce*	ROX	ce1;ce2Omy;ce3U;ce* Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated either after inhibition of CIII (e.g. antimycin A, myxothiazol), CIV (e.g. Cyanide) or in the absence of endogenous fuel-substrates. Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration. Succinate pathway control state (S-pathway) after inhibiting CI with rotenone, which also inhibits the F-pathway. The addition of rotenone or Antimycin A will depend on the experimental question to resolve.

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Coupling control

» Coupling control state

» ET capacity

» OXPHOS capacity

» LEAK respiration

Pathway control

» Electron transfer pathway

» Fatty acid oxidation pathway control state, F

» NADH electron transfer-pathway state, N

» Succinate pathway control state, S

» NS-pathway control state, NS

» Glycerophosphate pathway control state, Gp

» Complex IV single step, CIV

» Anaplerotic pathway control state

Main fuel substrates

» Glutamate, G

» Glycerophosphate, Gp

» Malate, M

» Octanoylcarnitine, Oct

» Pyruvate, P

» Succinate, S

Glossary

» List of SUIT states

» SUIT concept

@@ Line 24: / Line 24: @@
 *{{Template:SUIT L(Omy)}}
-*The addition of [[oligomycin]] or its carrier is optional depending on the experimental question to resolve.
+*The addition of [[oligomycin]] or its carrier (EtOH) is optional depending on the experimental question to resolve. If we add the carrier we do not reach LEAK state and we will evaluate if the EtOH alters the ROUTINE state.
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