Difference between revisions of "O2k-Open Support"

Latest revision as of 16:43, 9 August 2022

O2k-Open Support

Currently Asked Questions - »O2k-Open Support alert«

O2k-Open Support: technical help

O2k-Open Support includes Open Access pages that help to optimally use the O2k, to apply instrumental O2k-Quality Control, and perform user-service when needed. Support regarding data analysis and/or interpretation is provided on an individual basis, for which consultancy fees apply.

MitoPedia: O2k: find information quickly with MitoPedia, the Bioblast glossary with lists of key words and links to relevant pages.
O2k-Procedures: explain various applications of the O2k with O2k-Demo experiments
O2k-Manual: topic specific manuals with step by step help
O2k-Videosupport: provides valuable assistance, complementary to the O2k-Manual
O2k-Products: is not merely a 'List of products', it presents a guided tour to the O2k and integrates the concept of Gentle Science and Open Science.

O2k-Quality Control

Many commonly raised issues of O2k-technical support are solved in the newest version of DatLab.

The O₂ sensor and O2k chamber tests are important to eliminate instrumental artefacts (instrumental quality control, QC), distinguished from assay QC addressing problems of solution protocols (preparation of chemicals), sample preparation protocols (such as mitochondrial preparations), and SUIT protocols.

Clean O2k-chambers and contamination-free incubation medium are required.
Analysis of DatLab files recorded in instrumental QC tests is essential.
Biological experiments are not suitable for trouble shooting.

1. O₂ sensor test

The OroboPOS (polarographic oxygen sensor) requires regular service at intervals - these may be more than 1 year. SOPs are available to determine if a sensor service is required at an earlier or later date:

» O₂ sensor test: QC1: Oxygen sensor test

2. O2k-chamber test

If all quality control criteria of the O₂ sensor test are met, the operator can be assured that the quality of the sensor signal is acceptable. Next, the quality of the O2k-chamber assembly has to be tested, described in detail:

» O2k-chamber test: QC2: Instrumental O₂ background test

O2k-Open Support: Installation and startup support session

» MiPNet26.07 Installation and startup support session

Upon special request and subject to an additional fee, Oroboros Instruments offers a start-up visit by a scientific application specialist.

» O2k On-site Training

O2k-Open Support agreement

The Oroboros experts will be happy to help in using our O2k-Open Support system.

» Search for defective O2k components

» In case you need further technical support and for your valuable feedback, please contact O2k-Tech Support

» O2k-Open Support agreement

» O2k repair

The O2k-Network

The Oroboros O2k-Network serves to connect and support: Contact for advice on applications of the O2k and high-resolution respirometry - the global network in mitochondrial physiology.

» The O2k-Network Labs: » O2k-Network

» O2k-Network discussion forum

» Oroboros O2k-Laboratory - open for innovation and cooperation.

» Oroboros Science Scholarships - are offered for focused research projects on mitochondrial physiology and high-resolution respirometry

@@ Line 1: / Line 1: @@
-{{Mainpage OROBOROS}}
+{{Template:OROBOROS support page name}}
-[[Image:O2k-Info.png|right|80px|link=http://http://www.oroboros.at/?O2k|Info OROBOROS]]
+{{NextGen-O2k H2020-support}}
-[[Media:Trouble_shooting_presentation_2014.pdf|'''Open Access-Trouble_shooting.pdf''']]; [http://www.bioblast.at/index.php/File:Trouble_shooting_presentation_2014.pdf Versions]
+[[File:O2k-support system.jpg|right|350px|Oroboros support system]]
+:::::::::::: <big>''Currently Asked Questions - »'''[[O2k-Open Support alert]]'''« </big>
+__TOC__
+== O2k-Open Support: technical help ==
-'''O2k-Troubleshooting''' helps to localize and resolve technical problems encountered with the OROBOROS [[Oxygraph-2k]] in a very fast and cost effective approach.
+:::: '''O2k-Open Support''' includes [[Open Access]] pages that help to optimally use the O2k, to apply instrumental O2k-Quality Control, and perform user-service when needed. Support regarding data analysis and/or interpretation is provided on an individual basis, for which consultancy fees apply.
+::::::# '''MitoPedia: O2k''': find information quickly with MitoPedia, the Bioblast glossary with lists of key words and links to relevant pages.
-__TOC__
+::::::## '''[[MitoPedia: O2k-Open Support]]'''
-== Trouble shooting types ==
+::::::## [[MitoPedia: O2k hardware]]
-* 1. Distinction between '''application problems''' and technical hardware problems:
+::::::## [[MitoPedia: DatLab]]
-: An '''application problem''' can frequently be diagnosed on the basis of analysis of DatLab files recorded during O2k-calibration and instrumental background tests. By resolving such application problems, proper instrumental performance is obtained and a hardware problem is then excluded.
+::::::# [[O2k-Procedures]]: explain various applications of the O2k with O2k-Demo experiments
-: If an application problem or software problem (DatLab) can be excluded, the '''instrumental component''' causing the technical hardware problem has to be localized in Step 2.
+::::::# [[O2k-Manual]]: topic specific manuals with step by step help
+::::::# [[O2k-Videosupport]]: provides valuable assistance, complementary to the O2k-Manual
+::::::# [[O2k-Products]]: is not merely a 'List of products', it presents a guided tour to the O2k and integrates the concept of [[Gentle Science| Gentle Science and Open Science]].
-* 2. '''Technical trouble shooting''': Localization of the '''hardware component''' causing the technical problem. Specific diagnostic tests are described under 'O2k-Troubleshooting' which help to identify any defective instrumental component.
-* 3. Distinction between hardware problems that can be solved by '''user-service''' and hardware problems that require replacement of a technically defective part:
+::: '''<big>O2k-Quality Control</big>'''
-: The [[OroboPOS]] (polarographic oxygen sensor) requires user-service at intervals. Such user-service may solve specific technical problems.
+[[File:MitoFit-QCS.jpg|right|400px|MitoFit-QCS]]
-: If user-service is not an option to solve the technical problem, the diagnostic tests help to take an important decision in Step 4.
+:::: Many commonly raised issues of O2k-technical support are solved in the newest version of [[DatLab]].
-* 4. Distinction between '''hardware defects''' which can be solved by '''replacement of a defective spare part''' in contrast to electronic or mechanical problems which can be solved only by '''service of the Oxygraph-2k in the workshop of OROBOROS INSTRUMENTS'''.
+:::: The '''O<sub>2</sub> sensor and O2k chamber tests''' are important to eliminate instrumental artefacts (instrumental quality control, QC), distinguished from assay QC addressing problems of [[solution protocols]] (preparation of chemicals), [[sample]] preparation protocols (such as [[mitochondrial preparations]]), and [[SUIT protocols]].
-: Some specific potential problems, e.g. a non-functioning Peltier unit, can be detected directly by a single observation followed by specific instructions for this case, which then can be solved only by electronic service of the Oxygraph-2k in the OROBOROS workshop.
+::::# Clean O2k-chambers and contamination-free incubation medium are required.
+::::# Analysis of DatLab files recorded in instrumental QC tests is essential.
+::::# Biological experiments are not suitable for trouble shooting.
-Usually it is best to follow a general trouble shooting procedure, relating to a large variety of apparent technical problems. This is especially true for problems that may have different causes or are vague in their description. For example, "unstable signal", "no signal", "slow response" fall within this category.  The general O2k-Troubleshooting process described below will help to locate most hardware problems.
-== General O2k-Troubleshooting process ==
-To evaluate and diagnose hardware problems, instrumental tests are performed in the absence of any experimental sample, using an aqueous medium for which any microbiological contamination can be excluded. Experimental test runs containing a biological sample is generally not suitable for technical troubleshooting.
+=== 1. O<sub>2</sub> sensor test ===
+:::: The [[OroboPOS|OroboPOS]] (polarographic oxygen sensor) requires regular service at intervals - these may be more than 1 year. SOPs are available to determine if a sensor service is required at an earlier or later date:
+::::» O<sub>2</sub> sensor test: [[Oxygen_sensor_test |'''QC1: Oxygen sensor test''']]
-# Perform an OroboPOS [[Sensor test]] and analyze its results.
+=== 2. O2k-chamber test ===
-# Locate the problem utilizing the two-chamber approach of the O2k by strictly following the procedure described on the [[Locating a problem]] page. This also involves several more POS sensor tests.
+:::: If all quality control criteria of the O<sub>2</sub> sensor test are met, the operator can be assured that the quality of the sensor signal is acceptable. Next, the quality of the O2k-chamber assembly has to be tested, described in detail:
+::::» O2k-chamber test: [[Oxygen flux - instrumental background |'''QC2: Instrumental O<sub>2</sub> background test''']]
-== Specific problems that do not require a sensor test ==
-=== DatLab error messages ===
+== O2k-Open Support: Installation and startup support session ==
-* [[DatLab Error Message: Block temperature]]: ERROR: Average block temperature over past 10 min has not reached set temperature.
+:::::» '''[[MiPNet26.07 Installation and startup support session]]'''
-* [[DatLab Error Message: Line has wrong length]]: ERROR: Line has wrong length: This message appears when a signal line has not been transmitted properly from the Oxygraph-2k to DatLab.
-=== Other problems that do not require a POS sensor test ===
+:::: Upon special request and subject to an additional fee, Oroboros Instruments offers a start-up visit by a scientific application specialist.
+:::::» '''[[O2k On-site Training]]'''
-* Problems related only to the pX channel or the additional amperometric channel.
-* Jumping or blocked stirring bar.
-* [[Biological contamination | High oxygen flux at closed chamber]].
-== How to find O2k-Troubleshooting and technical service information in Bioblast ==
+[[File:O2k-Support.jpg|right|200px|O2k-Open Support]]
+== O2k-Open Support agreement ==
-* You can use the standard Wiki search function in the right upper corner of most pages.
+:::: The Oroboros experts will be happy to help in using our O2k-Open Support system.
-* Pages in the [[O2k-Catalogue_OROBOROS|O2k-Catalogue]] will frequentyl include a list of technical service pages related to the described component.
+::::» [[Search for defective O2k components]]
-* Use the list below to locate pages relevant to your problem.
+:::: '''»''' In case you need further technical support and for your valuable feedback, please contact [https://www.oroboros.at/index.php/o2k-technical-support '''O2k-Tech Support''']
+::::» [[O2k-Open Support agreement]]
+::::» [[O2k repair]]
-== Cost estimation for repair ==
-A cost  estimation for repair of the O2k-Main unit due to an  electronic/mechanical defect can only be given after inspection of the  O2k by our workshop in Austria. Once a cost estimation has been  provided, the repair is carried out after the customer’s consent. For  shipment of the O2k, please refer to the detailed [[Shipping an oxygraph  | shipping instructions]] on this troubleshooting site.
+[[File:O2k-Network.png|left|100px|O2k-Network|link=O2k-Network]]
+== The O2k-Network ==
-== All technical service and O2k-Troubleshooting pages ==
+:::: The Oroboros O2k-Network serves to connect and support: Contact for advice on applications of the O2k and high-resolution respirometry - the global network in mitochondrial physiology.
-{{#ask: mainlabel=Title|[[Category:All]] | [[Technical service::+]]
+::::: '''»''' The O2k-Network Labs: [[O2k-Network|» O2k-Network]]
-| ?Technical service
-| limit = 100
-}}
+::::: '''»''' [[O2k-Network_discussion_forum|O2k-Network discussion forum]]
+:::: '''»''' [[Oroboros Laboratory|Oroboros O2k-Laboratory]] - open for innovation and cooperation.
+:::: '''»''' [[Oroboros_Science_Scholarship|Oroboros Science Scholarships]] - are offered for focused research projects on mitochondrial physiology and high-resolution respirometry
-__SHOWFACTBOX__
+[[Category:O2k-Open Support]]
-{{Troubleshooting}}