Difference between revisions of "O2k-Open Support"

Latest revision as of 16:43, 9 August 2022

O2k-Open Support

Currently Asked Questions - »O2k-Open Support alert«

O2k-Open Support: technical help

O2k-Open Support includes Open Access pages that help to optimally use the O2k, to apply instrumental O2k-Quality Control, and perform user-service when needed. Support regarding data analysis and/or interpretation is provided on an individual basis, for which consultancy fees apply.

MitoPedia: O2k: find information quickly with MitoPedia, the Bioblast glossary with lists of key words and links to relevant pages.
O2k-Procedures: explain various applications of the O2k with O2k-Demo experiments
O2k-Manual: topic specific manuals with step by step help
O2k-Videosupport: provides valuable assistance, complementary to the O2k-Manual
O2k-Products: is not merely a 'List of products', it presents a guided tour to the O2k and integrates the concept of Gentle Science and Open Science.

O2k-Quality Control

Many commonly raised issues of O2k-technical support are solved in the newest version of DatLab.

The O₂ sensor and O2k chamber tests are important to eliminate instrumental artefacts (instrumental quality control, QC), distinguished from assay QC addressing problems of solution protocols (preparation of chemicals), sample preparation protocols (such as mitochondrial preparations), and SUIT protocols.

Clean O2k-chambers and contamination-free incubation medium are required.
Analysis of DatLab files recorded in instrumental QC tests is essential.
Biological experiments are not suitable for trouble shooting.

1. O₂ sensor test

The OroboPOS (polarographic oxygen sensor) requires regular service at intervals - these may be more than 1 year. SOPs are available to determine if a sensor service is required at an earlier or later date:

» O₂ sensor test: QC1: Oxygen sensor test

2. O2k-chamber test

If all quality control criteria of the O₂ sensor test are met, the operator can be assured that the quality of the sensor signal is acceptable. Next, the quality of the O2k-chamber assembly has to be tested, described in detail:

» O2k-chamber test: QC2: Instrumental O₂ background test

O2k-Open Support: Installation and startup support session

» MiPNet26.07 Installation and startup support session

Upon special request and subject to an additional fee, Oroboros Instruments offers a start-up visit by a scientific application specialist.

» O2k On-site Training

O2k-Open Support agreement

The Oroboros experts will be happy to help in using our O2k-Open Support system.

» Search for defective O2k components

» In case you need further technical support and for your valuable feedback, please contact O2k-Tech Support

» O2k-Open Support agreement

» O2k repair

The O2k-Network

The Oroboros O2k-Network serves to connect and support: Contact for advice on applications of the O2k and high-resolution respirometry - the global network in mitochondrial physiology.

» The O2k-Network Labs: » O2k-Network

» O2k-Network discussion forum

» Oroboros O2k-Laboratory - open for innovation and cooperation.

» Oroboros Science Scholarships - are offered for focused research projects on mitochondrial physiology and high-resolution respirometry

@@ Line 1: / Line 1: @@
-{{Technical service}}
+{{Template:OROBOROS support page name}}
-[[Image:O2k-Info.png|right|80px|link=OROBOROS info|OROBOROS info]]
+{{NextGen-O2k H2020-support}}
+[[File:O2k-support system.jpg|right|350px|Oroboros support system]]
+:::::::::::: <big>''Currently Asked Questions - »'''[[O2k-Open Support alert]]'''« </big>
+__TOC__
+== O2k-Open Support: technical help ==
+:::: '''O2k-Open Support''' includes [[Open Access]] pages that help to optimally use the O2k, to apply instrumental O2k-Quality Control, and perform user-service when needed. Support regarding data analysis and/or interpretation is provided on an individual basis, for which consultancy fees apply.
+::::::# '''MitoPedia: O2k''': find information quickly with MitoPedia, the Bioblast glossary with lists of key words and links to relevant pages.
+::::::## '''[[MitoPedia: O2k-Open Support]]'''
+::::::## [[MitoPedia: O2k hardware]]
+::::::## [[MitoPedia: DatLab]]
+::::::# [[O2k-Procedures]]: explain various applications of the O2k with O2k-Demo experiments
+::::::# [[O2k-Manual]]: topic specific manuals with step by step help
+::::::# [[O2k-Videosupport]]: provides valuable assistance, complementary to the O2k-Manual
+::::::# [[O2k-Products]]: is not merely a 'List of products', it presents a guided tour to the O2k and integrates the concept of [[Gentle Science| Gentle Science and Open Science]].
-'''O2k-technical service''' helps users of the OROBOROS [[Oxygraph-2k]] to localize and resolve technical problems encountered in high-resolution respirometry and O2k-MultiSensor applications - fast and cost-effectively.
-__TOC__
+::: '''<big>O2k-Quality Control</big>'''
-== O2k-technical service: categories ==
+[[File:MitoFit-QCS.jpg|right|400px|MitoFit-QCS]]
-To evaluate and diagnose technical problems, instrumental quality control tests are performed in the absence of experimental sample.
+:::: Many commonly raised issues of O2k-technical support are solved in the newest version of [[DatLab]].
-* Polarographic oxygen sensors: calibration, accuracy and quality control SOP. »[[MiPNet06.03 POS-Calibration-SOP]]«
+:::: The '''O<sub>2</sub> sensor and O2k chamber tests''' are important to eliminate instrumental artefacts (instrumental quality control, QC), distinguished from assay QC addressing problems of [[solution protocols]] (preparation of chemicals), [[sample]] preparation protocols (such as [[mitochondrial preparations]]), and [[SUIT protocols]].
+::::# Clean O2k-chambers and contamination-free incubation medium are required.
+::::# Analysis of DatLab files recorded in instrumental QC tests is essential.
+::::# Biological experiments are not suitable for trouble shooting.
+=== 1. O<sub>2</sub> sensor test ===
+:::: The [[OroboPOS|OroboPOS]] (polarographic oxygen sensor) requires regular service at intervals - these may be more than 1 year. SOPs are available to determine if a sensor service is required at an earlier or later date:
+::::» O<sub>2</sub> sensor test: [[Oxygen_sensor_test |'''QC1: Oxygen sensor test''']]
+=== 2. O2k-chamber test ===
+:::: If all quality control criteria of the O<sub>2</sub> sensor test are met, the operator can be assured that the quality of the sensor signal is acceptable. Next, the quality of the O2k-chamber assembly has to be tested, described in detail:
+::::» O2k-chamber test: [[Oxygen flux - instrumental background |'''QC2: Instrumental O<sub>2</sub> background test''']]
+== O2k-Open Support: Installation and startup support session ==
+:::::» '''[[MiPNet26.07 Installation and startup support session]]'''
+:::: Upon special request and subject to an additional fee, Oroboros Instruments offers a start-up visit by a scientific application specialist.
+:::::» '''[[O2k On-site Training]]'''
-=== Application problems ===
+[[File:O2k-Support.jpg|right|200px|O2k-Open Support]]
-An '''application problem''' can be diagnosed on the basis of O2k-calibration and instrumental background tests. Analysis of DatLab files recorded during instrumental tests is essential. If proper instrumental performance is obtained by resolving such application problems, any hardware problem is excluded.
+== O2k-Open Support agreement ==
-=== Software problems ===
+:::: The Oroboros experts will be happy to help in using our O2k-Open Support system.
-Communication between the O2k and DatLab running on a computer.
+::::» [[Search for defective O2k components]]
-: [[DatLab Error Message: Line has wrong length]]: Line has wrong length: This message appears when a signal line has not been transmitted properly from the Oxygraph-2k to DatLab. This is a problem only, if the error message appears frequently, and may indicate that the specifications of the laptop are not adequate for proper DatLab performance.
+:::: '''»''' In case you need further technical support and for your valuable feedback, please contact [https://www.oroboros.at/index.php/o2k-technical-support '''O2k-Tech Support''']
+::::» [[O2k-Open Support agreement]]
+::::» [[O2k repair]]
-=== Hardware problems ===
-Diagnostic tests are available to identify a defective instrumental component.
-* The two-chamber approach of the O2k provides the option to exchange parts between the two chambers and thus localize a defective component: »[[Locating a problem]]
-# Hardware problems that can be solved by user-service: The [[OroboPOS]] (polarographic oxygen sensor) requires user-service at intervals of more than 1 year, and SOPs are available to determine if a sensor service is required at an earlier or later date.
+[[File:O2k-Network.png|left|100px|O2k-Network|link=O2k-Network]]
-# Hardware problems that require replacement of a defective part.
+== The O2k-Network ==
-# Some electronic or mechanical defects may be solved only by service of the Oxygraph-2k in the workshop of OROBOROS INSTRUMENTS, e.g. a defective Peltier unit.
-: [[DatLab Error Message: Block temperature]]: Average block temperature over past 10 min has not reached set temperature.
+:::: The Oroboros O2k-Network serves to connect and support: Contact for advice on applications of the O2k and high-resolution respirometry - the global network in mitochondrial physiology.
-== Cost estimation for repair ==
+::::: '''»''' The O2k-Network Labs: [[O2k-Network|» O2k-Network]]
-A cost estimation for repair of the O2k-Main unit due to an  electronic/mechanical defect can only be given after inspection of the  O2k by our workshop in Austria. Once a cost estimation has been provided, the repair is carried out after the customer’s consent. For shipment of the O2k, follow the [[Shipping an oxygraph |detailed instructions]].
+::::: '''»''' [[O2k-Network_discussion_forum|O2k-Network discussion forum]]
+:::: '''»''' [[Oroboros Laboratory|Oroboros O2k-Laboratory]] - open for innovation and cooperation.
-== O2k-technical service pages ==
+:::: '''»''' [[Oroboros_Science_Scholarship|Oroboros Science Scholarships]] - are offered for focused research projects on mitochondrial physiology and high-resolution respirometry
-* Introduction to O2k-technical service: [[Media:Trouble_shooting_presentation_2014.pdf|'''»O2k-technical service.pdf''']]
-{{#ask: mainlabel=Title|[[Technical service::+]]
+[[Category:O2k-Open Support]]
-| ?Technical service
-| limit = 100
-}}