Oroboros O2k-technology
Description | Oroboros O2k - the modular system for high-resolution FluoRespirometry for mitochondria and cell research, using small amounts of biological samples. The O2k-FluoRespirometer is the basis for combined measurement of respiration and fluorometric signals, supported by the Titration-Injection microPump and DatLab 7 (new). The modular O2k-concept yields a high flexibility of FluoRespirometry with add-on O2k-MultiSensor Modules for research in mitochondrial physiology and pathology. |
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Product ID | 1#### |
Type | O2k, Catalogue |
Link | Oroboros O2k-Catalogue, Gnaiger_2008_POS. MiPNet06.01 O2k-Overview |
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- » 2-chamber high-resolution respirometer
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- » O2k-Dissection Set
- » Microbalance
- » O2k-Core
- » O2k-FluoRespirometer
- » O2k-MultiSensor Modules
- » PBI-Shredder O2k-Set
- » TIP2k-Module
O2k-Catalogue: O2k-Modules and Auxiliary Accessories
Oroboros O2k: development and distribution
- The O2k is developed by OROBOROS INSTRUMENTS in cooperation with WGT and produced in Austria by WGT.
- O2k-FluoRespirometry: HRR combined with measurement of ROS production, mitochondrial membrane potential, ATP production, Ca2+, pH.
- Titration-Injection microPump TIP2k: The O2k-TIP2k combination allows operation with programmable titration regimes, feedback control, and at steady-states, yielding a new flexibility in experimental design by combining the technical advantages of closed and open systems approaches. Automatic titrations for kinetics; feedback-controlled instrumental background tests; respiration in a closed or open system, in aerobic-anaerobic transitions or at steady-state oxygen concentrations, covering the entire physiological oxygen range and the extremes of hypoxia.
- O2k-Modules can be combined in any configuration with the O2k-FluoRespirometer, and can be added any time to extend the O2k according to application-specific objectives: potentiometry (pH, TPP+ or TPMP+ for mitochondrial membrane potential, Ca2+) and amperometry (NO, H2S).
- Auxiliary O2k-Tools provide extended support in the context of high-resolution respirometry and O2k-FluoRespirometry: Microsyringes, PBI-Shredder O2k-Set for tissue preparation; Microbalance, O2k-Dissection Set.
An Integrated Systems Approach: High-resolution FluoRespirometry
- The Oroboros O2k is the unique state-of-the-art instrument for HRFR - where high-resolution counts:
- with small amounts of cells or tissue,
- at low respiratory activities,
- multiple substrate-uncoupler-inhibitor titrations (SUIT protocols) with resolution of changes in oxygen flux over prolonged incubation times,
- flexibility of O2k-MultiSensor applications,
- fast transitions at low oxygen levels,
- stability and quality control.
- High-resolution respirometry is the result of our long-term expertise in instrumental design, software development, mitochondrial physiology, clinical and pharmacological applications, and world-wide scientific cooperation. The innovative O2k-developments provide a system of highest quality and distinguish high-resolution respirometry as a method and concept.
- Today, such high resolution meets rapidly increasing demands, reflected by the increasing number and high level of O2k-Publications. Applications of the O2k in leading O2k-Network Labs are the driving forces for a rapid advancement of mitochondrial physiology. Open access to the O2k-Manual is an integral part of the concept of OROBOROS INSTRUMENTS on Gentle Science and Scientific Social Responsibility. With sharing of O2k-Protocols and implementation of quality control, the study of mitochondrial and cell respiration emerges as a quantitative science. Thus high-resolution FluoRespirometry provides the foundation for comparative mitochondrial physiology, biomedical research, translation into clinical applications, establishing quantitative functional mitochondrial diagnosis.
- Sole source information: [|pdf]
- Frequently Asked Questions
- The Oroboros O2k sets a world-wide standard in high-resolution respirometry - for mitochondrial studies in cell physiology and pathology. Developments progress beyond oxygen.
- In contrast to merely offering an oxygen measuring device, the Oroboros O2k is integrated into a systems approach to high-resolution respirometry. This spans from experimental tools which are invaluable in the lab (e.g. ISS), or little aids that are crucial for tissue preparation (forceps), mitochondrial respration medium (MiR05-Kit), substrate-uncoupler-inhibitor titration protocols, and the unique O2k-Workshops.