SUIT-006: Difference between revisions
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:::+ The combinations of substrates for one ET-pathway state with inhibitors for other pathways provide the best coupling control protocols specififc for one ET-pathway state, e.g.: MnaPM for N-pathway control state, RotS for S-pathway control state. | :::+ The combinations of substrates for one ET-pathway state with inhibitors for other pathways provide the best coupling control protocols specififc for one ET-pathway state, e.g.: MnaPM for N-pathway control state, RotS for S-pathway control state. | ||
:::+ Short duration of the experiment. | :::+ Short duration of the experiment. | ||
:::- This protocol does not address questions regarding the convergence of pathways at the Q-junction. | |||
:::- Careful washing is required after the experiment to avoid carry-over of inhibitors and uncoupler. | :::- Careful washing is required after the experiment to avoid carry-over of inhibitors and uncoupler. | ||
:::- CIV activity is not measured, to save experimental time. | :::- CIV activity is not measured, to save experimental time. |
Revision as of 17:07, 17 January 2019
Description
[[File:|200px]]
Abbreviation: N, S, F, Gp
Reference: A ยปVersions
- SUIT-category: N, S, F, Gp
- SUIT protocol pattern: linear coupling control protocol 1SI;2D;3U;-
DLP applications
References
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1X | XL(n) | 1X
| ||
2D | XP | 1X;2D
| ||
2c | XcP | 1X;2D;2c
| ||
3Omy | XLOmy | 1X;2D;2c;3Omy
| ||
4U | XE | 1X;2D;2c;3Omy;4U
| ||
5Ama | ROX | 1X;2D;2c;3Omy;4U;5Ama
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- ยป Electron transfer pathway
- ยป Fatty acid oxidation pathway control state, F
- ยป NADH electron transfer-pathway state, N
- ยป Succinate pathway control state, S
- ยป NS-pathway control state, NS
- ยป Glycerophosphate pathway control state, Gp
- ยป Complex IV single step, CIV
- ยป Anaplerotic pathway control state
- Pathway control
- Main fuel substrates
- ยป Glutamate, G
- ยป Glycerophosphate, Gp
- ยป Malate, M
- ยป Octanoylcarnitine, Oct
- ยป Pyruvate, P
- ยป Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- This protocol is indicated when the aim is to analyse coupling control in mt preparations.
- Different combinations of substrates and inhibitors can be used depending on the specific aims, e.g.: PM, GM, MnaPM or other combinations for N-pathway control state; S or RotS for S-pathway control state.
- + The combinations of substrates for one ET-pathway state with inhibitors for other pathways provide the best coupling control protocols specififc for one ET-pathway state, e.g.: MnaPM for N-pathway control state, RotS for S-pathway control state.
- + Short duration of the experiment.
- - This protocol does not address questions regarding the convergence of pathways at the Q-junction.
- - Careful washing is required after the experiment to avoid carry-over of inhibitors and uncoupler.
- - CIV activity is not measured, to save experimental time.
Compare SUIT protocols
MitoPedia concepts: MiP concept, SUIT protocol, Recommended
MitoPedia methods:
Respirometry