From Bioblast
Description
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Abbreviation: N or S
Reference: A Β»Versions
- SUIT-category: N or S
- SUIT protocol pattern: linear coupling control protocol 1SI;2D;3U;-
DLP applications
References
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1X | XL(n) | 1X
| ||
2D | XP | 1X;2D
| ||
2c | XcP | 1X;2D;2c
| ||
3Omy | XLOmy | 1X;2D;2c;3Omy
| ||
4U | XE | 1X;2D;2c;3Omy;4U
| ||
5Ama | ROX | 1X;2D;2c;3Omy;4U;5Ama
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Β» Electron transfer pathway
- Β» Fatty acid oxidation pathway control state, F
- Β» NADH electron transfer-pathway state, N
- Β» Succinate pathway control state, S
- Β» NS-pathway control state, NS
- Β» Glycerophosphate pathway control state, Gp
- Β» Complex IV single step, CIV
- Β» Anaplerotic pathway control state
- Pathway control
- Main fuel substrates
- Β» Glutamate, G
- Β» Glycerophosphate, Gp
- Β» Malate, M
- Β» Octanoylcarnitine, Oct
- Β» Pyruvate, P
- Β» Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- This protocol is indicated when the aim is to analyse coupling control in mt preparations.
- Different combinations of substrates and inhibitors can be used depending on the specific aims, e.g.: PM, GM, MnaPM or other combinations for N-pathway control state; S or RotS for S-pathway control state.
- + The combinations of substrates for one ET-pathway state with inhibitors for other pathways provide the best coupling control protocols specififc for one ET-pathway state, e.g.: MnaPM for N-pathway control state, RotS for S-pathway control state.
- + Short duration of the experiment.
- - Careful washing is required after the experiment to avoid carry-over of inhibitors and uncoupler.
- - CIV activity is not measured, to save experimental time.
Compare SUIT protocols
MitoPedia concepts: MiP concept, SUIT protocol, Recommended
MitoPedia methods:
Respirometry