SUIT-023 O2 ce D053: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr= | |abbr=AOX-ce SHAM+CN | ||
|description=[[File:ce1;ce2SHAM;ce3KCN.png|350px]] | |description=[[File:ce1;ce2SHAM;ce3KCN.png|350px]] | ||
|info='''A''' Determination of the respiration due to the cytochrome ''c'' oxidase pathway -'''[[SUIT-023]]''' | |info='''A''' Determination of the respiration due to the cytochrome ''c'' oxidase pathway -'''[[SUIT-023]]''' | ||
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|SUIT number=D053_ce1;ce2SHAM;ce3KCN | |SUIT number=D053_ce1;ce2SHAM;ce3KCN | ||
}} | }} | ||
::: '''[[SUIT protocol pattern]]:''' ce1;ce2SHAM;ce3KCN | |||
::: '''[[SUIT protocol pattern]]:''' | |||
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The SUIT-023 O2 ce D053 provides an estimation of mitochondrial respiration in [[microalgal]] living cells through the CIII-cytochrome ''c'' oxidase pathway (CIII-CIV), which is inhibited by [[Antimycin_A| antimycin A]] and [[cyanide| cyanide]]. This protocol must be complemented with the study in parallel of the mitochondrial respiration through the Q-[[alternative oxidase]] pathway (see [[SUIT-022]]). | |||
__TOC__ | __TOC__ | ||
ย Communicated by [[Huete-Ortega M]] and [[Gnaiger E]] (last update 2019- | ย Communicated by [[Huete-Ortega M]] and [[Gnaiger E]] (last update 2019-06-15) | ||
{{Template:SUIT-023 O2 ce D053}} | {{Template:SUIT-023 O2 ce D053}} | ||
== Strengths and limitations == | == Strengths and limitations == | ||
:::* SUIT-023 O2 ce D053 provides an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-non-resistant mitochondrial respiration in [[microalgal]] | :::* SUIT-023 O2 ce D053 provides an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-non-resistant mitochondrial respiration in [[microalgal]] living cells. | ||
:::* The concentrations of inhibitors used in this protocol have been optimised for the specific case of the microalgal model species ''Chlamydomonas reinhardtii'' | :::* The concentrations of inhibitors used in this protocol have been optimised for the specific case of the microalgal model species ''Chlamydomonas reinhardtii'' | ||
:::+ Short duration of experiment | :::+ Short duration of experiment | ||
:::- | :::- No estimation of coupling control | ||
:::- The use of [[cyanide| cyanide]] requires | :::- The use of [[cyanide| cyanide]] requires careful cleaning of the chambers after use and careful handling of the chemical to guarantee health safety. | ||
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== Compare SUIT protocols == | == Compare SUIT protocols == | ||
::::* [[SUIT-022 O2 ce D051]] has to be used in parallel to obtain complete information of the contribution of | ::::* [[SUIT-022 O2 ce D051]] has to be used in parallel to obtain complete information of the contribution of the Q-AOX and CIII-CIV pathways to mitochondrial respiration. | ||
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Revision as of 08:56, 15 June 2019
Description
Abbreviation: AOX-ce SHAM+CN
Reference: A Determination of the respiration due to the cytochrome c oxidase pathway -SUIT-023
SUIT number: D053_ce1;ce2SHAM;ce3KCN
O2k-Application: O2
- SUIT protocol pattern: ce1;ce2SHAM;ce3KCN
The SUIT-023 O2 ce D053 provides an estimation of mitochondrial respiration in microalgal living cells through the CIII-cytochrome c oxidase pathway (CIII-CIV), which is inhibited by antimycin A and cyanide. This protocol must be complemented with the study in parallel of the mitochondrial respiration through the Q-alternative oxidase pathway (see SUIT-022).
Communicated by Huete-Ortega M and Gnaiger E (last update 2019-06-15)
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce1 | ROUTINE | ce1
| ||
ce2SHAM | ROUTINE OR ROX | ce1;ce2SHAM
| ||
ce3KCN | ROX | ce1;ce2SHAM;ce3KCN
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- ยป Electron transfer pathway
- ยป Fatty acid oxidation pathway control state, F
- ยป NADH electron transfer-pathway state, N
- ยป Succinate pathway control state, S
- ยป NS-pathway control state, NS
- ยป Glycerophosphate pathway control state, Gp
- ยป Complex IV single step, CIV
- ยป Anaplerotic pathway control state
- Pathway control
- Main fuel substrates
- ยป Glutamate, G
- ยป Glycerophosphate, Gp
- ยป Malate, M
- ยป Octanoylcarnitine, Oct
- ยป Pyruvate, P
- ยป Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- SUIT-023 O2 ce D053 provides an estimation of the cyanide-and antimycin A-non-resistant mitochondrial respiration in microalgal living cells.
- The concentrations of inhibitors used in this protocol have been optimised for the specific case of the microalgal model species Chlamydomonas reinhardtii
- + Short duration of experiment
- - No estimation of coupling control
- - The use of cyanide requires careful cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.
Compare SUIT protocols
- SUIT-022 O2 ce D051 has to be used in parallel to obtain complete information of the contribution of the Q-AOX and CIII-CIV pathways to mitochondrial respiration.
References
MitoPedia concepts: SUIT protocol, SUIT A, Find
MitoPedia methods:
Respirometry