Difference between revisions of "MiPNet06.03 POS-calibration-SOP"

Latest revision as of 14:27, 27 July 2023

MiPNet06.03 POS-calibration-SOP

O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration.

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Oroboros (2023-02-06) Mitochondr Physiol Network

Abstract: Gnaiger E (2023) O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration. Mitochondr Physiol Network 06.03(21):1-10.

Open the pdf document above.

» Current O2k-series: NextGen-O2k Series XB and O2k Series J

» Current software versions DatLab 8.0: MitoPedia: DatLab

Further details: » MitoPedia: O2k-Open Support

• O2k-Network Lab: AT_Innsbruck_Oroboros

Keywords

Bioblast links: Oxygen signal - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

Specific

O2k-Procedures

» High signal at zero oxygen

» POS calibration - static

» POS calibration - dynamic

MiPNet O2k-Procedures

» POS-calibration

» O2k-Manual: POS-service

» O2k-cleaning

» O2k-Series G and DatLab 6: Calibration

General

» Oxygen, dioxygen, O₂

» Oxygen calibration - DatLab

» Oxygen solubility

» Oxygen solubility factor

» Oxygen pressure

» Concentration

» Activity

» Pressure - Pascal

» Barometric pressure

» High-resolution respirometry

» OroboPOS

» Polarographic oxygen sensor

» MitoFit Quality Control System

» Sensitivity

Other keyword lists

» Keywords: Concentration and pressure

» Oxygen calibration - DatLab

Labels: MiParea: Respiration, Instruments;methods

HRR: Oxygraph-2k, O2k-Manual

DatLab, O2k-SOP, DL7, Oxygen solubility, DL8, MitoFit 2021 Dark respiration, MitoFit 2021 Photosynthesis, DL8, PLoSONE2022ace-sce, MitoFit2022QC, POS

@@ Line 1: / Line 1: @@
-{{OROBOROS header page name}}
+{{Template:OROBOROS support page name}}
 {{Publication
-|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] Polarographic oxygen sensors: calibration, accuracy and quality control SOP.
+|title=[[Image:O2k-Manual.jpg|right|70px|link=O2k-Manual|O2k-Manual]]O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration.
-|info=[[File:PDF.jpg|24px|link=http://wiki.oroboros.at/images/7/77/MiPNet06.03_POS-Calibration-SOP.pdf |Bioblast pdf]] » [http://www.bioblast.at/index.php/File:MiPNet06.03_POS-Calibration-SOP.pdf Versions - Open Access]
+|info=[[File:PDF.jpg|100px|link=https://wiki.oroboros.at/images/7/77/MiPNet06.03_POS-Calibration-SOP.pdf |Bioblast pdf]]  » [http://www.bioblast.at/index.php/File:MiPNet06.03_POS-Calibration-SOP.pdf Versions]
-|authors=OROBOROS
+|authors=Oroboros
-|year=2016-02-07
+|year=2023-02-06
 |journal=Mitochondr Physiol Network
-|abstract=Gnaiger E (2016) Polarographic oxygen sensors: calibration, accuracy and quality control SOP. Mitochondr Physiol Network 06.03(15):1-8.
+|abstract=Gnaiger E (2023) O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration. Mitochondr Physiol Network 06.03(21):1-10.
+{{MiPNet pdf page linking to MitoPedia}}
-[[High-resolution respirometry]] critically depends on accurate calibration of the [[polarographic oxygen sensor]]s which respond to [[Oxygen_pressure| partial oxygen pressure]].  Expressing the oxygen signal in terms of partial pressure has several advantages, but respiration is expressed in molar units related to biochemical stoichiometries. Conversion of oxygen partial pressure to oxygen concentration requires accurate information on oxygen solubilities in experimental incubation media. Absolute errors up to 10% are commonly found in the established literature. The [[oxygen solubility]] of mitochondrial respiration medium [[MiR06]] relative to pure water (oxygen solubility factor, ''F''<sub>M</sub>) is 0.92, accurately determined (for MiR05, i.e. MiR06 without catalase; [[MiPNet14.13]]) at 37 °C and 30 °C.  At air saturation at standard barometric pressure (100 kPa) and 37 °C, the partial oxygen pressure is 19.63 kPa, and the oxygen concentration is 190.7 µM in MiR06.  A clean and ready-to-use O2k-Chamber is a requirement for saving time and quickly calibrating the polarographic oxygen sensors.
+|mipnetlab=AT_Innsbruck_Oroboros
-:» O2k-Manual: »[[MiPNet19.18D_O2k-Calibration]]«
-:» Product: [[OroboPOS]], [[Oxygraph-2k|O2k]], [[OROBOROS O2k-Catalogue |O2k-Catalogue]]
-:» Template: [[File:O2k-Calibration-List.xls|O2k Calibration List]]
-|mipnetlab=AT_Innsbruck_OROBOROS
-}}
-{{Labeling
-|area=Instruments;methods
-|instruments=Oxygraph-2k, O2k-Protocol
-|additional=O2k-SOP
 }}
-{{Technical support integrated}}
+== Keywords ==
-[[File:O2 sensor test.jpg|right|400px|link=O2 sensor test |O2 sensor test]]
+{{Template:Keywords: Oxygen signal}}
-__TOC__
+::::» [[Oxygen calibration - DatLab]]
-== Further details ==
-* Next step of instrumental quality control: »[[MiPNet14.06 InstrumentalO2Background]]«
-* Forstner H, Gnaiger E (1983) Calculation of equilibrium oxygen concentration. In: Polarographic Oxygen Sensors. Aquatic and Physiological Applications. Gnaiger E, Forstner H (eds), Springer, Berlin, Heidelberg, New York:321-33. [[Forstner_1983_POS |»Bioblast Access«]]
+{{Labeling
-[[Image:MiPNet06.03_Table.jpg|centre|frame|<sup>c</sup>O2* is the oxygen concentration at thermodynamic equilibrium with air under standard conditions of 100 kPa barometric pressure. ''F''<sub>M</sub> has been determined for MiR05 at 30 °C and 37 °C, and is assumed to be identical at 25 °C.]]
+|area=Respiration, Instruments;methods
+|instruments=Oxygraph-2k, O2k-Manual
-== References ==
+|additional=DatLab, O2k-SOP, DL7, Oxygen solubility, DL8,
-* [[Gnaiger_2008_POS]]
+MitoFit 2021 Dark respiration, MitoFit 2021 Photosynthesis, DL8, PLoSONE2022ace-sce, MitoFit2022QC, POS
-* [[MiPNet19.18D_O2k-Calibration]]
+}}
-'''This article is cited in'''
-* Rodriguez JV, Pizarro MD, Scandizzi AL, Guibert EE, Almada LL, Mamprin ME (2008) Construction and performance of a minibioreactor suitable as experimental bioartificial liver. Artif Organs 32:323-8. [http://www.ncbi.nlm.nih.gov/pubmed/18370948 »PubMed 18370948«]
-[[Category:Technical service]]