Difference between revisions of "SUIT-003 O2 ce D028"
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|abbr=ceS | |abbr=ceS | ||
|description=[[File:ce1;ce2Omy;ce3U;ce4Rot;ce5S;ce6Ama.png|200px]] | |description=[[File:ce1;ce2Omy;ce3U;ce4Rot;ce5S;ce6Ama.png|200px]] | ||
|info='''A''' '''[[SUIT-003]]''' | |info='''A''' '''[[SUIT-003]]''' [[coupling control protocol]] with succinate for permeability test | ||
|SUIT number=D###ce1;ce2Omy;ce3U;ce4Rot;ce5S;ce6Ama | |SUIT number=D###ce1;ce2Omy;ce3U;ce4Rot;ce5S;ce6Ama | ||
|application=O2 | |application=O2 |
Revision as of 12:59, 21 January 2019
Description
Abbreviation: ceS
Reference: A SUIT-003 coupling control protocol with succinate for permeability test
SUIT number: D###ce1;ce2Omy;ce3U;ce4Rot;ce5S;ce6Ama
O2k-Application: O2
- SUIT-category: ceS
- SUIT protocol pattern: linear cell coupling control protocol ce1;ce2Omy;ce3U;ce4Rot;ce5S;ce6Ama
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce1 | ROUTINE | ce1
| ||
ce2Omy | L | ce1;ce2Omy
| ||
ce3U | E | ce1;ce2Omy;ce3U
| ||
ce4Rot | ROX | ce1;ce2Omy;ce3U;ce4Rot
|
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce5S | ce1;ce2Omy;ce3U;ce4Rot;ce5S
| |||
ce6Ama | ROX | ce1;ce2Omy;ce3U;ce4Rot;ce5S;ce6Ama
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Main fuel substrates
- Glossary
ceR,L,E format
In the diagrams of coupling control protocols for intact cells (CCP), the steps may be replaced by the respiratory states.
- ce4Rot,ce5S;ce6Ama
- ce4Rot: In many intact cells ROX measured after titration of rotenone (ce4Rot) is not or only slightly further inhibited by subsequent titration of antimycin A.
- ce5S: Succinate is not permeable through the plasma membrane of many intact cells (Steinlechner-Maran 1997 Transplantation). If succinate is accessible to rotenone-inhibited uncoupled mitochondria in dead cells (dce), respiration will be stimulated.
- ce6Ama: Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration (Stadlmann_2006_Cell Biochem Biophys).
MitoPedia concepts:
SUIT protocol,
SUIT A,
Find
MitoPedia methods:
Respirometry