SUIT-018 O2 mt D054: Difference between revisions
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ย Communicated by '''[[Komlodi T]]'''ย (last update 2019-03-05) | ย Communicated by '''[[Komlodi T]]'''ย (last update 2019-03-05) | ||
{{Template: SUIT-018 | {{Template: SUIT-018 O2_O2 Specific SUIT}} | ||
== Strengths and limitations == | == Strengths and limitations == |
Revision as of 09:33, 7 March 2019
Description
Abbreviation: NS(GM)
Reference: B: SUIT-018
SUIT number: D054_1GMS;2D;3Ama
O2k-Application: O2
- MitoPedia: SUIT
- SUIT-category: NS(GM)
- SUIT protocol pattern: lineal 1GMS;2D;3Ama
SUIT-018 O2 mt D054 is a short protocol to study the influence of the fluorescence dye on the O2 flux. During this protocol the [O2] can be modified injecting nitrogen in the chamber.
Communicated by Komlodi T (last update 2019-03-05)
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1GMS | GMSL(n) | NS | CI&CII | 1GMS
|
2D | GMSP | NS | CI&CII | 1GMS;2D
|
2c | GMScP | NS | CI&CII | 1GMS;2D;2c
|
3Ama | ROX | 1GMS;2D;2c;3Ama
|
Step | Respiratory state | Pathway control | ET-Complex | Comment |
---|---|---|---|---|
## AsTm | AsTmE | CIV | CIV | |
## Azd | CHB |
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- ยป Electron transfer pathway
- ยป Fatty acid oxidation pathway control state, F
- ยป NADH electron transfer-pathway state, N
- ยป Succinate pathway control state, S
- ยป NS-pathway control state, NS
- ยป Glycerophosphate pathway control state, Gp
- ยป Complex IV single step, CIV
- ยป Anaplerotic pathway control state
- Pathway control
- Main fuel substrates
- ยป Glutamate, G
- ยป Glycerophosphate, Gp
- ยป Malate, M
- ยป Octanoylcarnitine, Oct
- ยป Pyruvate, P
- ยป Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- This protocol can be used as a control for SUIT-018 AmR mt D031, SUIT-018 AmR mt D040 and SUIT-018 AmR mt D041. The oxygen concentration should be adjusted to the matching protocol with the Amplex UltraRed assay.
- + Reasonable duration of the experiment.
- - CIV activity and cytochrome c test can be performed.
Compare SUIT protocols
MitoPedia concepts: SUIT protocol, SUIT B, Find
MitoPedia methods:
Respirometry