Difference between revisions of "SUIT-018 O2 mt D054"
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::: '''[[Categories of SUIT protocols |SUIT-category]]:''' NS(GM) | ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' NS(GM) | ||
::: '''[[SUIT protocol pattern]]:''' lineal 1GMS;2D- | ::: '''[[SUIT protocol pattern]]:''' lineal 1GMS;2D- | ||
SUIT-018 O2 mt D054 is a short protocol to study the influence of | SUIT-018 O2 mt D054 is a short protocol to study the influence of a fluorescence dye on the O<sub>2</sub> flux. During this protocol the [O<sub>2</sub>] can be modified injecting nitrogen in the chamber. | ||
__TOC__ | __TOC__ |
Revision as of 13:29, 3 April 2019
Description
Abbreviation: NS(GM)
Reference: B: SUIT-018
SUIT number: D054_1GMS;2D;3Ama
O2k-Application: O2
- MitoPedia: SUIT
- SUIT-category: NS(GM)
- SUIT protocol pattern: lineal 1GMS;2D-
SUIT-018 O2 mt D054 is a short protocol to study the influence of a fluorescence dye on the O2 flux. During this protocol the [O2] can be modified injecting nitrogen in the chamber.
Communicated by Komlodi T and Gnaiger E (last update 2019-04-03)
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1GMS | GMSL(n) | NS | CI&CII | 1GMS
|
2D | GMSP | NS | CI&CII | 1GMS;2D
|
2c | GMScP | NS | CI&CII | 1GMS;2D;2c
|
3Ama | ROX | 1GMS;2D;2c;3Ama
|
Step | Respiratory state | Pathway control | ET-Complex | Comment |
---|---|---|---|---|
## AsTm | AsTmE | CIV | CIV | |
## Azd | CHB |
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- Coupling control
- Pathway control
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- This protocol can be used as a control for SUIT-018 AmR mt D031, SUIT-018 AmR mt D040 and SUIT-018 AmR mt D041. The oxygen concentration should be adjusted to the matching protocol with the Amplex UltraRed assay.
- + Reasonable duration of the experiment.
- - CIV activity and cytochrome c test can be performed.
Compare SUIT protocols
MitoPedia concepts: SUIT protocol, SUIT B, Find
MitoPedia methods:
Respirometry