SUIT-022: Difference between revisions
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|info='''A: Determination of the respiration due to the alternative oxidase pathway''' [[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/6/64/SUIT-022.pdf|Bioblast pdf]] ยป[http://wiki.oroboros.at/index.php/File:SUIT-022.pdf Versions] | |info='''A: Determination of the respiration due to the alternative oxidase pathway''' [[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/6/64/SUIT-022.pdf|Bioblast pdf]] ยป[http://wiki.oroboros.at/index.php/File:SUIT-022.pdf Versions] | ||
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::: '''[[Categories of SUIT protocols |SUIT-category]]:''' ce ย | ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' ce ย |
Revision as of 10:16, 15 May 2019
Description
Abbreviation: ce
Reference: A: Determination of the respiration due to the alternative oxidase pathway ยปVersions
- SUIT-category: ce
- SUIT protocol pattern: linear ce1;ce2KCN;ce3SHAM
The SUIT-022 is designed to provide an estimation of the mitochondrial respiration in cells due to the alternative oxidase pathway, which is cyanide-and antimycin A-resistant. This protocol must be complemented with the study in parallel of the mitochondrial respiration due to the cytochrome c oxidase (CIV) pathway (see SUIT-023).
Communicated by Huete-Ortega M and Gnaiger E (last update 2019-04-09)
Specific SUIT protocols
SUIT-022 O2 ce D051
- SUIT-022 O2 ce D051 for intact microalgal cells (ce)
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce1 | ROUTINE | ce1
| ||
ce2KCN | ROUTINE or ROX | ce1;ce2KCN
| ||
ce3SHAM | ROX | ce1;ce2KCN;ce3SHAM
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- ยป Electron transfer pathway
- ยป Fatty acid oxidation pathway control state, F
- ยป NADH electron transfer-pathway state, N
- ยป Succinate pathway control state, S
- ยป NS-pathway control state, NS
- ยป Glycerophosphate pathway control state, Gp
- ยป Complex IV single step, CIV
- ยป Anaplerotic pathway control state
- Pathway control
- Main fuel substrates
- ยป Glutamate, G
- ยป Glycerophosphate, Gp
- ยป Malate, M
- ยป Octanoylcarnitine, Oct
- ยป Pyruvate, P
- ยป Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- SUIT-022 provides an estimation of the cyanide-and antimycin A-resistant mitochondrial respiration due to the activity of the alternative oxidase on cells on ROUTINE. To complement the study with the estimation of the mitochondrial respiration due to the cytochrome c oxidase (CIV) pathway, it must be carried out in parallel to SUIT-023.
- + This protocol can be used with many type of cells, although optimal concentrations of inhibitors have to be optimised.
- + Short duration of experiment
- - Estimation of the linear coupling control is not done
- - The use of cyanide requires a careful and in deep cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.
Compare SUIT protocols
- SUIT-023 O2 ce D053 for microalgal intact cells
References
MitoPedia concepts: MiP concept, SUIT protocol, Recommended
MitoPedia methods:
Respirometry