SUIT-023 O2 ce D053: Difference between revisions
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ย Communicated by [[Huete-Ortega M]] and [[Gnaiger E]] (last update 2019-04-09) | ย Communicated by [[Huete-Ortega M]] and [[Gnaiger E]] (last update 2019-04-09) | ||
{{Template:SUIT- | {{Template:SUIT-023 O2 ce D053}} | ||
== Strengths and limitations == | == Strengths and limitations == |
Revision as of 15:45, 9 April 2019
Description
Abbreviation: ?
Reference: A Determination of the respiration due to the cytochrome c oxidase pathway -SUIT-023
SUIT number: D053_ce1;ce2SHAM;ce3KCN
O2k-Application: O2
- MitoPedia: SUIT
- SUIT-category: ce
- SUIT protocol pattern: linear ce1;ce2SHAM;ce3KCN
The SUIT-023 O2 ce D053 has been designed to provide an estimation of the mitochondrial respiration in microalgal intact cells due to the cytochrome c oxidase pathway, which is non-resistant to cyanide-and antimycin A inhibition. This protocol must be complemented with the study in parallel of the mitochondrial respiration due to the alternative oxidase pathway (see SUIT-022).
Communicated by Huete-Ortega M and Gnaiger E (last update 2019-04-09)
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce1 | ROUTINE | ce1
| ||
ce2SHAM | ROUTINE OR ROX | ce1;ce2SHAM
| ||
ce3KCN | ROX | ce1;ce2SHAM;ce3KCN
|
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- Coupling control
- Pathway control
- ยป Electron transfer pathway
- ยป Fatty acid oxidation pathway control state, F
- ยป NADH electron transfer-pathway state, N
- ยป Succinate pathway control state, S
- ยป NS-pathway control state, NS
- ยป Glycerophosphate pathway control state, Gp
- ยป Complex IV single step, CIV
- ยป Anaplerotic pathway control state
- Pathway control
- Main fuel substrates
- ยป Glutamate, G
- ยป Glycerophosphate, Gp
- ยป Malate, M
- ยป Octanoylcarnitine, Oct
- ยป Pyruvate, P
- ยป Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- SUIT-023 O2 ce D053 provides an estimation of the cyanide-and antimycin A-non-resistant mitochondrial respiration in microalgal intact cells.
- The concentrations of inhibitors used in this protocol have been optimised for the specific case of the microalgal model species Chlamydomonas reinhardtii
- + Short duration of experiment
- - Estimation of the linear coupling control is not done
- - The use of cyanide requires a careful and in deep cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.
Compare SUIT protocols
- SUIT-022 O2 ce D051 has to be used in parallel to obtain complete information of the contribution of both alternative oxidase and cytochrome c oxidase pathways to mitochondrial respiration.
References
MitoPedia concepts: SUIT protocol, SUIT A, Find
MitoPedia methods:
Respirometry