Template:SUIT-014: Difference between revisions
No edit summary |
No edit summary |
||
Line 14: | Line 14: | ||
| GM<sub>''L''</sub> or GM_L: Glutamate & malate, N-LEAK respiration, N<sub>''L''</sub> | | GM<sub>''L''</sub> or GM_L: Glutamate & malate, N-LEAK respiration, N<sub>''L''</sub> | ||
{{Template:SUIT N}} {{Template:SUIT L n}} | {{Template:SUIT N}} {{Template:SUIT L(n)}} | ||
|- | |- |
Revision as of 08:33, 25 January 2019
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1GM | GML | N | CI | GML or GM_L: Glutamate & malate, N-LEAK respiration, NL
NADH-linked substrates (type N-pathway to Q). Non-phosphorylating resting state (LEAK state); LEAK respiration L(n) in the absence of ADP, ATP, AMP (no adenylates). |
2D | GMP | N | CI | 1GM;2D
NADH-linked substrates (type N-pathway to Q). OXPHOS capacity P (with saturating [ADP]), active OXPHOS state. |
3P | PGMP | N | CI | 1GM;2D;3P
NADH-linked substrates (type N-pathway to Q). OXPHOS capacity P (with saturating [ADP]), active OXPHOS state. |
4S | PGMSP | NS | CI&II | 1GM;2D;3P;4S
Respiratory stimulation by simultaneous action of type N substrates & succinate, with convergent electron flow in the NS-pathway for reconstitution of TCA cycle function. OXPHOS capacity P (with saturating [ADP]), active OXPHOS state. |
5U | PGMSE | NS | CI&II | 1GM;2D;3P;4S;5U
Respiratory stimulation by simultaneous action of type N substrates & succinate, with convergent electron flow in the NS-pathway for reconstitution of TCA cycle function. Uncoupler titration (avoiding inhibition by high uncoupler concentrations) to obtain electron transfer (ET) capacity E (noncoupled ET-state). Test for limitation of OXPHOS capacity P by the phosphorylation system (ANT, ATP synthase, phosphate transporter) relative to ET capacity E in mt-preparations: E-P control efficiency and E-L coupling efficiency. In living cells: E-R control efficiency and E-L coupling efficiency. Noncoupled electron transfer state, ET state, with ET capacity E. |
6Rot | SE | S | CII | 1GM;2D;3P;4S;5U;6Rot
Succinate, S ( type S-pathway to Q). Noncoupled electron transfer state, ET state, with ET capacity E. |
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- ยป Electron transfer pathway
- ยป Fatty acid oxidation pathway control state, F
- ยป NADH electron transfer-pathway state, N
- ยป Succinate pathway control state, S
- ยป NS-pathway control state, NS
- ยป Glycerophosphate pathway control state, Gp
- ยป Complex IV single step, CIV
- ยป Anaplerotic pathway control state
- Pathway control
- Main fuel substrates
- ยป Glutamate, G
- ยป Glycerophosphate, Gp
- ยป Malate, M
- ยป Octanoylcarnitine, Oct
- ยป Pyruvate, P
- ยป Succinate, S
- Main fuel substrates
- Glossary