Difference between revisions of "SUIT-004 O2 pfi D010"
From Bioblast
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== Strengths and limitations == | == Strengths and limitations == | ||
:::*A succinate concentration of >10 mM may be required for saturating SE capacity. | :::* A succinate concentration of >10 mM may be required for saturating SE capacity. | ||
::::* CIV assessment is skipped if a prolonged protocol is not practical due to time limitation. | |||
::::# '''RP1-6Ama''' may be skipped since correction of the initial states by ROX measured in RP1-6 Ama may represent an over-correction, and inhibition takes a very long time in several cases ([[Pesta 2012 Methods Mol Biol]]). | |||
:::+ Linear coupling control (L-P-E) with N substrates (PM). | :::+ Linear coupling control (L-P-E) with N substrates (PM). | ||
:::+ Pathway control in ET state (N, NS and S pathways). | :::+ Pathway control in ET state (N, NS and S pathways). | ||
:::+ This protocol can be extended with the Complex IV module. | :::+ This protocol can be extended with the Complex IV module. | ||
:::+ Short experimental time. | :::+ Short experimental time. | ||
:::- The substrate combination for maximum ET capacity (FNSGP) is not obtained in SUIT-004 in favor of reducing the | :::- The substrate combination for maximum ET capacity (FNSGP) is not obtained in SUIT-004 in favor of reducing the time consumed during the experiment. | ||
== Compare SUIT protocols == | == Compare SUIT protocols == | ||
:::* [[SUIT-001 O2 pfi D002]] | |||
:::'''Specific cases''' | :::'''Specific cases''' | ||
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::::* [[Permeabilized muscle fibres |pfi]], high O<sub>2</sub>, no ROX: NS(PM)01_pfiO2,1PM,2D(c),3U,4S,5Rot | ::::* [[Permeabilized muscle fibres |pfi]], high O<sub>2</sub>, no ROX: NS(PM)01_pfiO2,1PM,2D(c),3U,4S,5Rot | ||
{{MitoPedia concepts | {{MitoPedia concepts |
Revision as of 16:58, 30 January 2019
Description
Abbreviation: NS(PM)
Reference: A: SUIT-004 short protocol linked to SUIT-001 O2 pfi D002 - SUIT RP1 (human skeletal muscle)
SUIT number: D010_1PM;2D;2c;3U;4S;5Rot;6Ama;7AsTm;8Azd
O2k-Application: O2
- SUIT-category: NS(PM)
- SUIT protocol pattern: orthogonal 1PM;2D;2c;3U;4S;5Rot
- Linked to SUIT-001 O2 pfi D002 - SUIT RP1, specifically for human skeletal muscle mitochondria.
- Harmonized with SUIT-005 O2 pfi D011.
Communicated by Iglesias-Gonzalez J and Gnaiger E (last update 2019-01-21)
References
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1PM | PML(n) | N | CI | 1PM
|
2D | PMP | N | CI | 1PM;2D
|
2c | PMcP | N | CI | 1PM;2D;2c
|
3U | PME | N | CI | 1PM;2D;3U
|
4S | PMSE | NS | CI&II | 1PM;2D;3U;4S
|
5Rot | SE | S | CII | 1PM;2D;3U;4S;5Rot
|
6Ama | ROX | 1PM;2D;3U;4S;5Rot;6Ama
|
Step | Respiratory state | Pathway control | ET-Complex | Comment |
---|---|---|---|---|
## AsTm | AsTmE | CIV | CIV | |
## Azd | CHB |
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- A succinate concentration of >10 mM may be required for saturating SE capacity.
- CIV assessment is skipped if a prolonged protocol is not practical due to time limitation.
- RP1-6Ama may be skipped since correction of the initial states by ROX measured in RP1-6 Ama may represent an over-correction, and inhibition takes a very long time in several cases (Pesta 2012 Methods Mol Biol).
- + Linear coupling control (L-P-E) with N substrates (PM).
- + Pathway control in ET state (N, NS and S pathways).
- + This protocol can be extended with the Complex IV module.
- + Short experimental time.
- - The substrate combination for maximum ET capacity (FNSGP) is not obtained in SUIT-004 in favor of reducing the time consumed during the experiment.
Compare SUIT protocols
MitoPedia concepts: SUIT protocol, SUIT A, Find
MitoPedia methods:
Respirometry