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  • ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' ce ::: '''[[SUIT protocol pattern]]:''' ce1
    2 KB (278 words) - 09:38, 9 November 2021
  • |info='''A''' '''[[SUIT-013]]''' |SUIT number=D023_ce1
    2 KB (277 words) - 11:31, 7 March 2024
  • ...mR assay components on the respiration ; ce: non-permeabilized cells -'''[[SUIT-003]]''' |SUIT number=D058_ce1;ce1SOD;ce1HRP;ce1AmR;ce2Omy;ce3U;ce4Rot;ce5Ama
    2 KB (328 words) - 16:06, 8 June 2020
  • |info='''B:''' Experimental control for [[SUIT-003 AmR ce D058]] |SUIT number=D059_ce1;ce1H2O;ce1MiR05;ce1DMSO;ce2Omy;ce3U;ce4Rot;ce5Ama
    2 KB (309 words) - 13:44, 7 July 2020
  • |info='''A''' -'''[[SUIT-018]]''' - SUIT protocol for simultaneous determination of O<sub>2</sub> and H<sub>2</sub>O |SUIT number=D068_ce1;1Dig;1GMS;2D;3Ama
    3 KB (430 words) - 12:06, 7 March 2024
  • ...s (isolated mitochondria, tissue homogenate and permeabilized cells)- '''[[SUIT-032]]''' |SUIT number=D109_mt;1PGM;2D;3Myx
    2 KB (226 words) - 09:56, 26 January 2024
  • ...s (isolated mitochondria, tissue homogenate and permeabilized cells)- '''[[SUIT-006]]''' |SUIT number=D108_mt;1PGM;2D;2c;(3Omy);4U;5Myx
    2 KB (233 words) - 10:43, 26 January 2024
  • ...s (isolated mitochondria, tissue homogenate and permeabilized cells)- '''[[SUIT-034]]''' |SUIT number=D082_mt;1D.1;2PGM;3D2.5;4U;5Myx
    2 KB (244 words) - 09:56, 26 January 2024
  • ...s (isolated mitochondria, tissue homogenate and permeabilized cells)- '''[[SUIT-033]]''' |SUIT number=D110_mt;1D.1;2PGM;3D2.5;4Myx
    2 KB (238 words) - 09:57, 26 January 2024
  • :::: [[SUIT protocol pattern]]: 1PM;2D;3U;4S;5Rot- ...ccinate pathway control state| S]]). SUIT-004 can be extended with the CIV assay module.
    2 KB (293 words) - 23:36, 12 April 2021
  • |abbr=SUIT ...rates states induced by multiple titrations within a single experimental [[assay]].
    3 KB (339 words) - 08:57, 27 May 2020
  • ::::## [[Assay]], Experimental assay ...[[Solution protocols]]: media, substrates, uncouplers, inhibitors used in SUIT protocols, permeabilization agents, etc.
    2 KB (222 words) - 06:12, 19 July 2022
  • |info='''A''' -'''[[SUIT-018]]''' |SUIT number=D041_mt;1GMS;2D;3Ama
    3 KB (397 words) - 12:08, 7 March 2024
  • |info='''A''' -'''[[SUIT-018]]''' - SUIT protocol for simultaneous determination of O<sub>2</sub> and H<sub>2</sub>O |SUIT number=D031_mt;1GMS;2D;3Ama
    4 KB (498 words) - 12:09, 7 March 2024
  • ::: '''[[SUIT protocol pattern]]:''' 1PM;2D;2c;3G;4U- ...anaplerotic pathway control state]]. SUIT-012 can be extended with the CIV assay module.
    4 KB (505 words) - 23:57, 12 April 2021
  • ::: '''[[SUIT protocol pattern]]:''' 1OctM;2D;3G;4P;5S;6U:7Rot- ...pacity| ET state]] can be evaluated. SUIT-015 can be extended with the CIV assay module.
    3 KB (393 words) - 15:21, 8 June 2020
  • |description=[[File:1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp-.png|400px|SUIT-001]] |mitopedia concept=MiP concept, SUIT protocol, Recommended
    5 KB (713 words) - 16:38, 5 March 2024
  • |mitopedia concept=MiP concept, SUIT protocol, Recommended ::: '''[[SUIT protocol pattern]]:''' 1OctM;2D;3P;4S;5U;6Rot-
    3 KB (424 words) - 14:30, 10 March 2023
  • ::: '''[[SUIT protocol pattern]]:'''1S;2D;3P;4Rot- ...e H<sub>2</sub>O<sub>2</sub> flux. The sensitivity of the Amplex UltraRed® assay (for determining H<sub>2</sub>O<sub>2</sub> production) changes over the ex
    6 KB (864 words) - 23:56, 12 April 2021
  • |description=[[File:1S;2D;3P;4Rot;5Ama.png|400px|SUIT-009]] ...t: isolated mitochondria, tissue homogenate and permeabilized cells - '''[[SUIT-009]]'''short protocol for H<sub>2</sub>O<sub>2</sub> (AmR) for isolated mi
    5 KB (681 words) - 00:11, 13 April 2021
  • ...s (isolated mitochondria, tissue homogenate and permeabilized cells)- '''[[SUIT-032]]''' |SUIT number=D078_File:1mt;1PGM;2D;3Anox;4Myx;5Reox
    3 KB (442 words) - 10:37, 12 January 2024
  • ::: '''[[SUIT protocol pattern]]:''' 1D;2M;3Oct;4M;5P;6G;7S;8Gp;9U;10Rot- ...aluated by using this SUIT protocol. SUIT-002 can be extended with the CIV assay module.
    6 KB (809 words) - 18:41, 5 March 2024
  • ...s (isolated mitochondria, tissue homogenate and permeabilized cells)- '''[[SUIT-033]]''' |SUIT number=D081_mt;1D.1;2PGM;3D2.5;4Anox;5Myx;6Reox
    3 KB (462 words) - 17:44, 4 January 2024
  • ...s (isolated mitochondria, tissue homogenate and permeabilized cells)- '''[[SUIT-034]]''' |SUIT number=D082_mt;1D.1;2PGM;3D2.5;4U;5Anox;6Myx;7Reox
    3 KB (454 words) - 16:56, 21 December 2023
  • ...'''A''' Linear coupling control with NADH-linked substrates for pce - '''[[SUIT-012]]''' |SUIT number=D052_ce1;1Dig;1PM;2D;2c;3G;4U;5Ama
    3 KB (412 words) - 00:14, 13 April 2021
  • ...s (isolated mitochondria, tissue homogenate and permeabilized cells)- '''[[SUIT-006]]''' |SUIT number=D084_mt;1PGM;2D;3(Omy);4U;5Anox;6Myx;7Reox
    3 KB (462 words) - 17:23, 21 December 2023
  • ...for isolated mitochondria, tissue homogenate and permeabilized cells- '''[[SUIT-012]]''' |SUIT number=D027_1PM;2D;2c;3G;4U;5Ama
    3 KB (397 words) - 00:12, 13 April 2021
  • |description=[[File:1GM;2D;3S;4U;5Rot-.png|400px|SUIT-011]] ::: '''[[SUIT protocol pattern]]:''' 1GM;2D;2c;3S;4U;5Rot-
    6 KB (951 words) - 15:35, 8 June 2020
  • ...IT protocol for mt; mt: isolated mitochondria and tissue homogenate - '''[[SUIT-008]]''' |SUIT number=D026_1PM;2D;2c;3G;4S;4D;5U;6Rot;7Ama;8AsTm;9Azd
    3 KB (433 words) - 10:56, 17 September 2020
  • ::: '''[[SUIT protocol pattern]]:''' 1PM;2D;3G;4S;5U;6Rot- ...relevant in some pathologies. SUIT-008 can be easily extended with the CIV assay module.
    4 KB (668 words) - 09:41, 30 November 2023
  • |description=[[File:1GMS;2D-.png|300px|SUIT-018]] ::: '''[[SUIT protocol pattern]]:''' 1GMS;2D;-
    5 KB (778 words) - 23:47, 12 April 2021
  • ...'A: quality control evaluation of [[mitochondrial preparations]]''' - '''[[SUIT-029]]''' |SUIT number=D066_mt;1PM;2T;2D;2c;3Omy;4U;5G;6S;6U;7Rot;8Ama
    4 KB (508 words) - 11:50, 15 March 2024
  • ...are reliable and reproducible (Figure 6A). Interlaboratory ring testing of reference samples is known as an external quality control (Figure 6B). The need for s ...a prototype test scenario and expanding the range of application-specific SUIT protocols in the MitoFit-data base. O2k-Core applications are extended by a
    3 KB (444 words) - 13:26, 3 April 2022
  • ...eux 2017 Sci Rep]] - '''[[SUIT-008]]'''- O2 pfi D014 protocol was used a a reference protocol in [[MitoEAGLE]] project (WG2) to evaluate the researcher skills p |SUIT number=D014_1PM;2D;2c;3G;4S;4D;5U;6Rot;7Ama;8AsTm;9Azd
    4 KB (539 words) - 23:48, 12 April 2021
  • ...sub> (AmR) in ce: non-permeabilized cells, pce: permeabilized cells -'''[[SUIT-009]]''' |SUIT number=D019_ce1;1Dig;1S;2D;3P;4Rot;5Ama
    5 KB (832 words) - 00:10, 13 April 2021
  • ::: '''MitoPedia: SUIT''' - '''Substrate-uncoupler-inhibitor titration protocols''' :::<big> » The '''[[SUITbrowser]] helps to find the best SUIT protocol for your research questions'''.</big>
    9 KB (1,166 words) - 19:56, 11 February 2023
  • ::: '''[[SUIT protocol pattern]]:''' 1ce;2ceOmy;3ceU;ce*, or ce1;ce2Omy;ce3U;ce4Rot;ce5S; ...em]] has to be studied to avoid an underestimation of the [[ET capacity]]. SUIT-003 follows a modular approach and can be easily used in combination with t
    6 KB (974 words) - 13:11, 17 November 2023
  • ...dardized respiratory SUIT protocols combined with SOPs in the fluorometric assay of H<sub>2</sub>O<sub>2</sub> production offer a sensitive diagnostic tool ==Reference==
    4 KB (511 words) - 18:41, 10 January 2022
  • |description=[[File:1GMS;2D;3Ama.png|290px|SUIT-018]] ...ns (isolated mitochondria, tissue homogenate and permeabilized cells)-'''[[SUIT-018]]'''
    4 KB (625 words) - 12:09, 7 March 2024
  • ...s (isolated mitochondria, tissue homogenate and permeabilized cells)- '''[[SUIT-006]]''' |SUIT number=D048_1PM;2D;3Omy;4U;5Ama
    4 KB (615 words) - 23:51, 12 April 2021
  • ...ferent respiratory control states, normalized for maximum flux in a common reference state, to obtain theoretical lower and upper limits of 0.0 and 1.0 (0 % and ...ometric incubation with sequential titrations (sequential protocol; [[SUIT|SUIT protocol]]) provide an internal normalization, expressing respiratory contr
    3 KB (427 words) - 10:40, 24 October 2023
  • |abbr=SUIT-catg |description=[[File:SUIT-catg_MitoPathway types.jpg|right|200px]]
    5 KB (673 words) - 16:25, 3 June 2020
  • |info='''A: for cells-permeabilized cells''' [[Lemieux 2017 Sci Rep]] - '''[[SUIT-008]]''' |SUIT number=D025_ce1;1Dig;1PM;2D;2c;3G;4S;5U;6Rot;7Ama;8AsTm;9Azd
    3 KB (516 words) - 23:58, 12 April 2021
  • ...BIOPS for 1 h at 37 °C. Different substrate-uncoupler-inhibitor titration (SUIT) protocols were used for diagnostic OXPHOS analysis. Using inhibitors of th ...dardized respiratory SUIT protocols combined with SOPs in the fluorometric assay of H<sub>2</sub>O<sub>2</sub> production offer a sensitive diagnostic tool
    5 KB (653 words) - 18:48, 10 January 2022
  • |title=[[File:SobotkaO.JPG|left|90px|Ondrej Sobotka]] Reference protocols for ROS production measurements. ...y control. SUIT protocols give us this opportunity with recently developed Reference protocols 1&2 for a wide use in comparative mitochondrial physiology.
    3 KB (362 words) - 14:24, 5 July 2023
  • |info='''A''' pfi: permeabilized fibers - '''[[SUIT-011]]''' |SUIT number=D024_1GM;2D;2c;3S;4U;5Rot;6Ama
    5 KB (784 words) - 11:18, 17 September 2020
  • ...s (isolated mitochondria, permeabilized cells, and tissue homogenate)-'''[[SUIT-021]]''' |SUIT number=D036_1GM;2D;3S;4Rot;5Omy;6U;7Ama
    6 KB (834 words) - 14:07, 6 September 2021
  • The fluorometric Amplex UltraRed AmR assay is frequently used for quantitative assessment of hydrogen peroxide product ...'') the medium specificity of the background fluorescence slope of the AmR assay, and (''2'') the oxygen dependence of H<sub>2</sub>O<sub>2</sub> flux in ba
    5 KB (700 words) - 13:33, 1 May 2024
  • ...s (isolated mitochondria, tissue homogenate and permeabilized cells) -'''[[SUIT-006]]''' |SUIT number=D055_1PM;2D;3Cat;4U;5Ama
    5 KB (755 words) - 10:43, 18 August 2023
  • ...2</sub> flux and mitochondrial ATP production in permeabilized cells -'''[[SUIT-006]]''' |SUIT number=D085_ce1;1Dig;1PM;2D;3Cat;4U;5Ama
    5 KB (762 words) - 11:12, 18 August 2023
  • ...respiratory control by coupling and mitochondrial pathways, SUIT reference assay.''' |[[Gnaiger 2014 MitoPathways|The Blue Book]] - [[SUIT reference protocol|SUIT reference protocol]]
    7 KB (1,004 words) - 11:59, 27 May 2019
  • ...have to be overcome, providing a rationale for establishing a set of basic reference protocols. ...Science Camp 2016]]: Optimizing strategies on the malate concentration in SUIT protocols.
    3 KB (424 words) - 13:26, 3 April 2022
  • ...g|left|90px|Calabria Elisa]] PBMCS purification and mitochondrial function assay: monitoring preparative steps and comparison of protocols. ...of the main aim of [[WG4]] is also protocols in the harmonization to SUIT reference protocols
    2 KB (295 words) - 14:00, 27 March 2018
  • ...xperience with the MitoKit-CII compounds, and specifically explain the new SUIT-protocols and limitations of application of the MitoKit-CII compounds. == Specific SUIT protocols ==
    5 KB (694 words) - 11:51, 17 August 2022
  • ...e extended, when further respiratory states (e.g. cell viability test; CIV assay) are added to the coupling control module consisting of three coupling cont ...POS]], [[Gnaiger 2020 BEC MitoPathways]], [[BEC 2020.1]], [[SUIT-003]], [[SUIT-006]]
    6 KB (874 words) - 10:49, 30 October 2023
  • ...chemicals within a single experimental assay. The application of complex [[SUIT]] protocols and handling multiple O2k-Instruments simultaneously will becom ...strumental development of automated O2k-Titration Robot for application in SUIT protocols.
    5 KB (598 words) - 15:02, 15 February 2024
  • ...with N-linked substrates. Both protocols were designed to provide a common reference state of NS-linked ET capacity, in comparison to separate N- and S-linked p
    4 KB (530 words) - 18:38, 10 January 2022
  • ...g|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] The following SUIT protocols are specifically optimized for PBMC and platelets <br>
    21 KB (2,889 words) - 13:31, 19 January 2021
  • ...]]), total flux ''E''´ (not corrected for ''Rox''), should be taken as the reference. ''Rox'' may be related to, but is of course different from [[ROS]] product ...ion, which may be evaluated by a direct measurement within an experimental assay, or by independent test experiments for critical evaluation of the best est
    10 KB (1,409 words) - 08:36, 25 November 2023
  • ...ce flux for normalization. The reference flux is determined in a metabolic reference state (1) as internal mt-marker (Gnaiger 2020), ...data''': The default [[data recording interval]] is 2 s, applied with most SUIT protocols. The larger the data recording interval, the lower is the time re
    17 KB (2,751 words) - 09:55, 17 November 2023
  • |description=[[File:SUIT-catg FNSGpCIV.jpg|right|400px]] ...te|ET-pathway competent states]], including oxygen supply. [[Categories of SUIT protocols]] are defined according to mitochondrial ET-pathway states.
    15 KB (1,913 words) - 19:27, 25 August 2023
  • ...ith CI-linked substrates. Both protocols were designed to provide a common reference state of CI&II-linked ET capacity, in comparison to separate Complex I- and
    5 KB (640 words) - 18:36, 10 January 2022
  • ...a<sup>2+</sup> and fuel substrates, were carried over into the respiratory assay from the serum in PA. A simple washing step was sufficient to enable functi |?Has title=Reference
    4 KB (612 words) - 16:09, 4 June 2022
  • ...ith CI-linked substrates. Both protocols were designed to provide a common reference state of CI<small>&</small>II-linked ET capacity, in comparison to separate
    5 KB (671 words) - 18:35, 10 January 2022
  • ...ith CI-linked substrates. Both protocols were designed to provide a common reference state of CI<small>&</small>II-linked ET-capacity, in comparison to separate
    5 KB (657 words) - 18:46, 10 January 2022
  • ...not in the LEAK state. In this [[flux control efficiency]], CHNOc is the [[reference state]] with stimulated flux; CHNO is the [[background state]] with CHNO su ...Acta |»Bioblast link«]]</ref>,<ref>Kuznetsov AV, Gnaiger E (2010) Oxygraph assay of cytochrome ''c'' oxidase activity: chemical background correction. Mitoc
    11 KB (1,496 words) - 11:35, 21 January 2022
  • ...ing an experiment due to AmR consumption and dilution by titrations in a [[SUIT protocol]]. Check for any potential effects of the experimental AmR concent ...aRed assay, and in the same respiration medium, the sensitivity of the AmR assay should be in the same range. For more information: [[Komlodi 2018 Methods M
    35 KB (5,265 words) - 15:10, 25 April 2024
  • ...pling hypothesis was based on a substrate-uncoupler-inhibitor titration ([[SUIT]]) protocol using high-resolution respirometry designed to interrogate coup ...is expressed as the change of flux from ''Y'' to ''Z'', normalized for the reference state ''Z'':
    10 KB (1,454 words) - 18:29, 10 January 2022
  • [[File:SUIT-nomenclature.jpg|right|400px|link= MitoPedia: SUIT|SUIT protocols]] === A1: Diagnostic SUIT protocols of mitochondrial fitness ===
    34 KB (4,472 words) - 11:55, 12 March 2024
  • ...ack into the open chamber. However, if the chamber is opened without prior SUIT steps, the medium in the stopper capillary does not contain cells and dilut ...index ''VI''<sub>ce</sub> should be equal or higher than 0.95 in a healthy reference cell population. Normalization for the cell count of the total of living ce
    35 KB (5,607 words) - 16:01, 21 February 2024
  • ::::* 16:30-17:00 SUIT design and the reproducibility crisis: open discussion on quality control ( ...cts and artefacts in measurements of H2O2 production using Amplex UltraRed assay as a function of oxygen pressure » [[Media:Timea Komlodi_april19.pdf |Abst
    33 KB (4,250 words) - 15:07, 2 February 2022
  • |?Has title=Reference ...certain types of clinical studies. - I would add that the selection of the reference for normalization is driven by the question being asked by a particular stu
    22 KB (3,436 words) - 00:28, 30 January 2024
  • ::::* Citations in the text <ref> Reference </ref> ::* Kuznetsov AV, Gnaiger E (2010) Oxygraph assay of cytochrome ''c'' oxidase activity: chemical background correction. Mitoc
    15 KB (2,011 words) - 20:19, 1 January 2021
  • ...ide production under hypoxia: Facts and artefacts with the Amplex UltraRed assay '''<br> ...to measure H<sub>2</sub>O<sub>2</sub> production using the Amplex UltraRed assay in combination with high-resolution respirometry.'''<br>
    85 KB (11,819 words) - 14:18, 10 January 2022
  • | Training in the Oroboros MitoFit Laboratory – from SUIT protocols to interpretation and reporting ...resolution respirometry, O2k-Fluorometry; MitoEAGLE proficiency test, SUIT reference protocols; isolation of mitochondria from various tissues, intact and perme
    41 KB (5,420 words) - 15:06, 23 November 2020
  • ...round H<sub>2</sub>O<sub>2</sub> calibration in the Amplex® UltraRed (AmR) assay:'''<br> ...d fluorescence in our Excel analysis template, and (''2'') calculation AmR assay sensitivity, for quality control. <br>
    126 KB (17,785 words) - 08:55, 13 August 2021
  • ...ub> fluxes in different respiratory states to the maximum flux in a common reference state. <br> |'''Protocols that SUIT your research question'''<br>
    80 KB (11,165 words) - 09:37, 8 January 2024
  • ...g|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] |''' What are the substrate-uncoupler-inhibitor titration reference protocols?'''<br>
    87 KB (11,941 words) - 15:14, 16 November 2023
  • ...etabolism, provides the basis for substrate-uncoupler-inhibitor titration (SUIT) protocols, and updates the terminology consistent with the MitoEAGLE white ...ariables on flow or flux can be expressed by normalization for rate in a ''reference state'', and is evaluated relative to a ''background state''. The concept o
    89 KB (12,074 words) - 17:54, 6 May 2024
  • ...mitochondria (imt) casts doubts on the validity of measurements in the pfi assay (oxygen limitation?). :::: '''Critical comments (with reference to lack of further evidence) or validation of isolated mitochondria by comp
    52 KB (7,111 words) - 14:50, 13 January 2023