Oroboros Ecosystem Agenda 2021 Archive

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Oroboros Ecosystem Agenda 2021 Archive

Mitochondria and Cell Research - the Oroboros Ecosystem
» Current Oroboros Ecosystem Agenda

Last week

Sep 2021

2021 Week 35 

  • Sep-01 We
O2k-Publications in the MiPMap Impaired mitochondrial function in skeletal muscle fibers of a presymptomatic COVID-19 patient

from the O2k-Network »US UT Salt Lake City Richardson R« 

  • Trinity JD, Craig JC, Fermoyle CC, McKenzie AI, Lewis MT, Park SH, Rondina MT, Richardson RS (2021) Impact of presymptomatic COVID-19 on vascular and skeletal muscle function: a case study. J Appl Physiol (1985) 130:1961-70. »Bioblast link«

  • Sep-02 Th

  • Sep-03 Fr

  • Sep-04 Sa - World Leukemia Day
O2k-brief repository Mitochondrial respiration of chronic lymphocytic leukemia cells can serve as a biomarker for treatment response
from the O2k-Network »CA Winnipeg Banerji V«
  • Chowdhury SR, Peltier C, Hou S, Singh A, Johnston JB, Gibson SB, Marshall A, Banerji V (2021) Ex vivo mitochondrial respiration parallels biochemical response to ibrutinib in CLL cells. Cancers (Basel) 13:E354. »Bioblast link«
 2021 Week 36 
  • Sep-06 Mo
Importance of mitochondrial fitness in viral infections - exercise as a protective factor for COVID-19
from the O2k-Network »CH Lausanne Place N« and  »AT Innsbruck Burtscher M« 
  • Burtscher J, Burtscher M, Millet GP (2021) The central role of mitochondrial fitness on antiviral defenses: An advocacy for physical activity during the COVID-19 pandemic. Redox Biol 43:1-13. »Bioblast link«

  • Sep-07 Tu
FAT4BRAIN »FAT4BRAIN Online Workshop: Brain energy metabolism in emotion and cognition«

  • Sep-08 We

  • Sep-09 Th
O2k-Open SupportInfo: improve Can I compare Amplex UltraRed (AmR) experiments in which the H2O2 sensitivity is different?

The H2O2 sensitivity is calculated to calibrate the raw fluorescence signal to H2O2 equivalent concentration. Using the same gain and fluorescence intensity it is possible to compare experiments with different sensitivities.
- »AmR assay H2O2 sensitivity«
- communicated by »Cardoso Luiza« and »Komlodi Timea«

  • Sep-10 Fr
2021 Week 37
  • Sep-13 Mo
»10th Int CeBiTec Research Conference 2021 Bielefeld DE« Sep 13-15

  • Sep-14 Tu
O2k-brief repository Remodeling of OXPHOS in prostate cancer to potentially damaging mtDNA mutations and differential expression of mt-genes
from the O2k-Network »AT Innsbruck Gnaiger E« and »AT Innsbruck Oroboros« 
  • Schöpf Bernd, Weissensteiner Hansi, Schäfer Georg, Fazzini Federica, Charoentong Pornpimol, Naschberger Andreas, Rupp Bernhard, Fendt Liane, Bukur Valesca, Giese Irina, Sorn Patrick, Sant’Anna-Silva Ana Carolina, Iglesias-Gonzalez Javier, Sahin Ugur, Kronenberg Florian, Gnaiger Erich, Klocker Helmut (2020) OXPHOS remodeling in high-grade prostate cancer involves mtDNA mutations and increased succinate oxidation. Nat Commun 11:1487. »Bioblast link«

  • Sep-15 We

  • Sep-16 Th

  • Sep-17 Fr
»EMC2021 Prague CZ« Sep 17-20

Aug 2021

2021 Week 31
  • Aug-02 Mo

  • Aug-03 Tu
O2k-Publications in the MiPMap Increased NS-linked flux control ratios in liver mitochondria of hyperbilirubinemic rats associated with increased mitochondrial biogenesis

from the O2k-Network »AU Southport Peart J« and »AU Queensland Neuzil J«

  • Vidimce J, Pillay J, Shrestha N, Dong LF, Neuzil J, Wagner KH, Holland OJ, Bulmer AC (2021) Mitochondrial function, fatty acid metabolism, and body composition in the hyperbilirubinemic gunn rat. Front Pharmacol 12:586715. »Bioblast link«

  • Aug-04 We

  • Aug-05 Th
O2k-Open SupportInfo: improve What is the advantage of using flux control ratios (FCRs)?

FCRs provide information on coupling and pathway control of mitochondrial respiration independent of (i) mt-content in cells or tissues, (ii) purification of isolated mitochondrial preparations, and (iii) determination of tissue mass or mt-markers.
- »Flux control ratio«
- communicated by »Cardoso Luiza« and »Cecatto Cristiane«

  • Aug-06 Fr
 2021 Week 32
  • Aug-09 Mo

  • Aug-10 Tu
O2k-Publications in the MiPMap Kinetic parameters of AOX proteins from Candida spp. with respect to ubiquinol-10 in a proteoliposome system – a step towards treatment development.
from the O2k-Network »UK Brighton Moore AL«
  • Copsey AC, Barsottini MRO, May B, Xu F, Albury MS, Young L, Moore AL (2021) Kinetic characterisation and inhibitor sensitivity of Candida albicans and Candida auris recombinant AOX expressed in a self-assembled proteoliposome system. Sci Rep 11:14748. »Bioblast link«

  • Aug-11 We

  • Aug-12 Th
O2k-Open Support Take care Do you know how to minimize the dilution of the AmR assay components upon sample addition?

Learn more about our recommendations:
- » AmR assay dilution «
- communicated by »Komlodi Timea« and »Cecatto Cristiane«

  • Aug-13 Fr
2021 Week 33  
  • Aug-16 Mo

  • Aug-17 Tu
O2k-Publications in the MiPMap A link between decreased P-L control efficiency and higher mortality of septic shock patients

from the O2k-Network »BR Rio de Janeiro Galina A« 

  • Nedel WL, Kopczynski A, Rodolphi MS, Strogulski NR, De Bastiani M, Montes THM, Abruzzi J Jr, Galina A, Horvath TL, Portela LV (2021) Mortality of septic shock patients is associated with impaired mitochondrial oxidative coupling efficiency in lymphocytes: a prospective cohort study. Intensive Care Med Exp 9:39. »Bioblast link«

  • Aug-18 We
Abstract submission deadline for »SHVM 2021 Virtual«

  • Aug-19 Th
O2k-Open SupportWarning: loss of data Which chemicals can I use in an experiment with Amplex UltraRed (AmR)?

Certain substances are incompatible due to their effect on the AmR assay sensitivity. Testing this effect before use is recommended.
- »Chemical compatibility with AmR«
- communicated by »Cardoso Luiza« and »Cecatto Cristiane«

  • Aug-20 Fr
2021 Week 34
  • Aug-23 Mo
O2k-Publications in the MiPMap Combined aerobic and resistance training improves mitochondrial respiration in obese women

from the O2k-Network »DK Copenhagen Larsen S« 

  • Mendham AE, Goedecke JH, Zeng Y, Larsen S, George C, Hauksson J, Fortuin-de Smidt MC, Chibalin AV, Olsson T, Chorell E (2021) Exercise training improves mitochondrial respiration and is associated with an altered intramuscular phospholipid signature in women with obesity. Diabetologia 64:1642-59. »Bioblast link«

  • Aug-24 Tu
FAT4BRAIN  »FAT4BRAIN Advanced O2k-Workshop IOC150 Virtual«  August 24-25

  • Aug-25 We
Bioenergetics Communications Magnesium Green™ (MgG) enables measurement of ATP production without affecting respiration
  • Cardoso LHD, Doerrier C, Gnaiger E (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. Bioenerg Commun 2021.1. doi:10.26124/bec:2021-0001

  • Aug-26 Th
O2k-Open SupportInfo: improve Is it normal to see a pink coloring in the medium at the end of an experiment with Amplex UltraRed (AmR)?

The pink coloring is due to the accumulation of UltroxRed, generated from Amplex UltraRed reacting with H2O2.
- »AmR assay and color of the medium«
- communicated by »Cardoso Luiza« and »Komlodi Timea«

  • Aug-27 Fr

  • Aug-28 Sa

  • Aug-29 Su
»Chlamy 2021 Ile des Embiez FR« Aug 29 - Sep 3

 2021 Week 35 
  • Aug-30 Mo
Bioenergetics Communications Steps towards Open Science – from preprint to BEC manuscript with published Open Peer Reviews

BEC follows a concept of living communications with peer-reviews published non-anonymously.
- »Bioenergetics Communications«

  • Aug-31 Tu
Payment deadline for »SHVM 2021 Virtual«

Jul 2021

  • Jul-01 Th
18:00-19:00 (GMT+2): Oroboros Instruments at ISAP 2021 Virtual - Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry

  • Jul-02 Fr
O2k-brief repository Inflammation - a cause of mitochondrial dysfunction and hepatic cell death in mice fed a high-fat diet
from the O2k-Network »US MO St Louis Gross RW«
  • Moon SH, Dilthey BG, Liu X, Guan S, Sims HF, Gross RW (2021) High-Fat diet activates liver iPLA2γ generating eicosanoids that mediate metabolic stress. J Lipid Res 62: 100052. [Epub ahead of print]. »Bioblast link«
2021 Week 27
  • Jul-05 Mo
O2k-Publications in the MiPMap Early adipocyte hypertrophy is accompanied with decreased mitochondrial oxygen consumption and increased ROS formation
from the O2k-Network »DE Munich Zischka H«
  • Baldini F, Fabbri R, Eberhagen C, Voci A, Portincasa P, Zischka H, Vergani L (2021) Adipocyte hypertrophy parallels alterations of mitochondrial status in a cell model for adipose tissue dysfunction in obesity. Life Sci 265:118812. »Bioblast link«

  • Jul-06 Tu

  • Jul-07 We
TPP+ for potentiometric detection of mt-membrane potential (mtMP) or fluorometry with safranin?

Safranin enables measurement of the relationship between fluorescence intensity and mtMP, and if linear, the absolute mtMP can be calculated. TPP+ enables calibration for measurement of mtMP.
- »TPP+ vs safranin«

  • Jul-08 Th

  • Jul-09 Fr
2021 Week 28
  • Jul-12 Mo
O2k-Publications in the MiPMap 4000 O2k-Publications reached!

Well over 1000 researchers have contributed to this achievement. Send us your O2k-Publication to be added to the list.
- »O2k-Publications«

  • Jul-13 Tu
O2k-Publications in the MiPMap Dietary octanoate cannot rescue cardiomyopathy in CPT2 deficiency

from the O2k-Network »US PA Pittsburgh Goetzman ES«

  • Pereyra AS, Harris KL, Soepriatna AH, Waterbury QA, Bharathi SS, Zhang Y, Fisher-Wellman KH, Goergen CJ, Goetzman ES, Ellis JM (2021) Octanoate is differentially metabolized in liver and muscle and fails to rescue cardiomyopathy in CPT2 deficiency. J Lipid Res 62:100069 »Bioblast link«

  • Jul-14 We

  • Jul-15 Th
NextGen-O2k »NextGen-O2k Event Photobiology: Algal bioenergetics Innsbruck AT« Jul 15-16

  • Jul-16 Fr
O2k-brief repository Doxorubicin-transferrin conjugate alters mitochondrial bioenergetics in breast cancer cells
from the O2k-Network »PL Lodz Watala C«
  • Wigner P, Zielinski K, Labieniec-Watala M, Marczak A, Szwed M (2021) Doxorubicin-transferrin conjugate alters mitochondrial homeostasis and energy metabolism in human breast cancer cells. Sci Rep 11:4544. »Bioblast link«
 2021 Week 29
  • Jul-19 Mo

  • Jul-20 Tu
O2k-Publications in the MiPMap Athletic conditioning in Alaskan racing sled dogs results in decreased calculated efficiency of skeletal muscle mitochondria.

from the O2k-Network »US OK Stillwater Davis MS«

  • Davis MS, Barrett MR (2021) Effect of conditioning and physiology hyperthermia on canine skeletal muscle mitochondrial oxygen consumption. J Appl Physiol. 130:1317-1325 »Bioblast link«

  • Jul-21 We
MitoPedia: SUIT Not sure which SUIT protocol to apply?

The SUITbrowser is the perfect tool to find the best SUIT protocol for your research question.
- » SUITbrowser« and »O2k-Videosupport: How to find a DL-Protocol«

- communicated by »Schmitt Sabine« and »Komlodi Timea«

  • Jul-22 Th

  • Jul-23 Fr
2021 Week 30
  • Jul-26 Mo

  • Jul-27 Tu

  • Jul-28 We
O2k-Open SupportInfo: improve Need to tailor a SUIT protocol to answer your research question?

See how to modify existing DL-protocols.
- »Enable_DL-Protocol_editing«
- communicated by »Schmitt Sabine«, »Cecatto Cristiane«, and »Komlodi Timea«

  • Jul-29 Th
O2k-Open SupportInfo: improve Do you know how to export a user-specific DL-protocol (*.DLPU)?

- »Export_DL-Protocol_User_(*.DLPU)« and »O2k-Videosupport:Export DL-Protocol User«
- communicated by »Schmitt Sabine«, »Cecatto Cristiane«, and »Komlodi Timea«

  • Jul-30 Fr

Jun 2021

2021 Week 22

  • Jun-01 Tu
O2k-Publications in the MiPMap Mitochondrial transplantation improves survival and bacterial clearance in a cecal slurry model of sepsis
  • Hwang JW, Lee MJ, Chung TN, Lee HAR, Lee JH, Choi SY, Park YJ, Kim CH, Jin I, Kim SH, Kwak HB, Heo JW, Na K, Choi S, Choi YS, Kim K (2021) The immune modulatory effects of mitochondrial transplantation on cecal slurry model in rat. Crit Care 25:20. »Bioblast link«

  • Jun-02 We

  • Jun-03 Th
O2k-Open SupportWarning: loss of data How to ensure quality control when exchanging the 2-mL and the 0.5-mL chambers

Every time you exchange the chambers, a volume calibration and instrumental O2 background test should be performed.
- »MiPNet22.11 Section 5.4. O2k-chamber volume calibration« and »Oxygen flux - instrumental background«
- communicated by »Komlodi Timea« and »Cecatto Cristiane«

  • Jun-04 Fr
 2021 Week 23
  • Jun-07 Mo
O2k-Open SupportWarning: loss of data Do you observe high fluorescence signal even before adding the dye or sample?

Filters that have shifted from the correct position in the Fluo-Sensors might give rise to higher fluorescence signal than usual.
- »Troubleshooting: fluorescence module« 
- communicated by »Komlodi Timea« and »Schmitt Sabine«

  • Jun-08 Tu
FAT4BRAIN »FAT4BRAIN Online Workshop: Central regulatory mechanisms of energy metabolism«

  • Jun-09 We
ESCI.JPG ESCI 2021 Virtual Jun 9-11
Komlodi Timea presenting at ESCI 2021 Virtual: Coenzyme Q redox state and high-resolution respirometry: Novel perspectives for mitochondrial diagnostics

  • Jun-10 Th
O2k-Publications in the MiPMap Mitoquinone does not alter respiration and mt-membrane potential in obese and diabetic rats.
from the O2k-Network »US IA Iowa City Sivitz WI« 
  • Fink BD, Yu L, Coppey L, Obrosov A, Shevalye H, Kerns RJ, Yorek MA, Sivitz WI (2021) Effect of mitoquinone on liver metabolism and steatosis in obese and diabetic rats. Pharmacol Res Perspect 9:e00701. »Bioblast link«

  • Jun-11 Fr
2021 Week 24
  • Jun-14 Mo
FAT4BRAIN  »FAT4BRAIN Advanced O2k-Workshop IOC149 Virtual« 

  • Jun-15 Tu

  • Jun-16 We
»World summit on Diabetes 2021 Virtual« June 16-17
O2k-Open SupportInfo: improve Simultaneous fluctuations in O2 slope neg. and the signal of the Peltier power?

This can indicate an electrical disturbance and changing the electrical supply may resolve this problem.
- »Troubleshooting«
- communicated by »Cecatto Cristiane« and »Schmitt Sabine«

  • Jun-17 Th
11:00-11:30 am (GMT+2): Gnaiger Erich at World summit on Diabetes 2021 Virtual - Succinate Anaplerosis has an Onco-Driving potential in Prostate Cancer Cells

  • Jun-18 Fr
FAT4BRAIN »FAT4BRAIN 2nd Online ESR Workshop« 
O2k-brief repository Effects of sodium butyrate on mitochondrial respiration in colon cancer cells
from the O2k-Network »EE Tallinn Kaambre T«
  • Klepinina L, Klepinin A, Truu L, Chekulayev V, Vija H, Kuus K, Teino I, Pook M, Maimets T, Kaambre T (2021) Colon cancer cell differentiation by sodium butyrate modulates metabolic plasticity of Caco-2 cells via alteration of phosphotransfer network. PLoS One 16:e0245348. »Bioblast link«

  • Jun-19 Sa
TRANSMIT Acronym.jpg »TRANSMIT final meeting 2021 Virtual«
2021 Week 25
  • Jun-21 Mo

  • Jun-22 Tu
O2k-Publications in the MiPMap Downregulation of mitoNEET causes cardiac impairment in aging
from the O2k-Network »JP Sapporo Yokota T« 
  • Furihata T, Takada S, Kakutani N, Maekawa S, Tsuda M, Matsumoto J, Mizushima W, Fukushima A, Yokota T, Enzan N, Matsushima S, Handa H, Fumoto Y, Nio-Kobayashi J, Iwanaga T, Tanaka S, Tsutsui H, Sabe H, Kinugawa S (2021) Cardiac-specific loss of mitoNEET expression is linked with age-related heart failure. Commun Biol 4:138. »Bioblast link«

  • Jun-23 We

  • Jun-24 Th
Do you know how to select the correct fluorescence sensors and filter set?

Using different fluorescence sensors, LED and photodiode filters, the O2k-Fluo Smart-Module (or O2k-Fluo LED2-Module) enables measurement of hydrogen peroxide, ATP, mitochondrial membrane potential, calcium, and numerous other applications open for O2k-user innovation.
- »Selecting the Smart Fluo-Sensor« and »Selecting a Filter Set«

  • Jun-25 Fr
2021 Week 26 
  • Jun-28 Mo
O2k-Open SupportInfo: improve Do I need to calibrate the fluorescence signal with safranin?

It is possible to run the experiment without calibration, presenting only the fluorescence intensity plot. However, a simple two-point or multiple-point calibration for safranin concentration is possible.
- »Safranin calibration«

  • Jun-29 Tu

  • Jun-30 We

May 2021

2021 Week 18
  • May-03 Mo
MitoFit pdf Exploring photosynthesis and dark respiration in the microalgae Chlamydomonas reinhardtii

Measurement of oxygen dependence with High-Resolution PhotoRespirometry:

  • Went N, Di Marcello M, Gnaiger E (2021) Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry. MitoFit Prep 2021.5. doi:10.26124/mitofit:2021-0005

  • May-04 Tu
O2k-Open SupportInfo: improve Do you know how to apply the air and zero calibration values in DatLab?

This important quality control step ensures accurate measurement of respiration.
For details on how to apply air and zero calibration in DatLab, see:
»Oxygen calibration - DatLab« and »Troubleshooting« 
- communicated by »Komlodi Timea« and »Cecatto Cristiane«

  • May-05 We
O2k-Publications in the MiPMap Succinate: a key regulator of mitochondrial function and HIF-1α upregulation in prostate cancer
from the O2k-Network »AT Innsbruck Oroboros«, »AT Innsbruck Gnaiger E«
  • Weber A, Klocker H, Oberacher H, Gnaiger E, Neuwirt H, Sampson N, Eder IE (2018) Succinate accumulation is associated with a shift of mitochondrial respiratory control and HIF-1α upregulation in PTEN negative prostate cancer cells. Int J Mol Sci 19:2129. »Bioblast link«

  • May-06 Th

  • May-07 Fr
O2k-brief repository Cell-permeable succinate can restore statin-inhibited mitochondrial respiration
from the O2k-Network »RO Timisoara Muntean DM« and »SE Lund Elmer E«
  • Avram VF, Chamkha I, Åsander-Frostner E, Ehinger JK, Timar RZ, Hansson MJ, Muntean DM, Elmér E (2021) Cell-permeable succinate rescues mitochondrial respiration in cellular models of statin toxicity. Int J Mol Sci 22:424. »Bioblast link«
 2021 Week 19
  • May-10 Mo
Abstract submission deadline for »EMC2021 Prague CZ«

  • May-11 Tu
MitoPedia: SUIT Measuring succinate pathway (S-pathway) in living cells

Using MitoKit-CII – cell permeable succinate and malonate, the S-pathway can be measured
- »SUIT-003 O2 ce D060«
- communicated by »Cecatto Cristiane« and »Komlodi Timea« 

  • May-12 We
O2k-Publications in the MiPMap Does prenatal hypoxia cause sex-dependent effects of mitochondrial function?
from the O2k-Network »UK Manchester Galli GL«
  • Hellgren KT, Premanandhan H, Quinn CJ, Trafford AW, Galli GLJ (2020) Sex-dependent effects of developmental hypoxia on cardiac mitochondria from adult murine offspring. Free Radic Biol Med [Epub ahead of print]. »Bioblast link«

  • May-13 Th
»SfRBM 2021 Virtual« May 13-14

  • May-14 Fr
»ISAP 2021 Virtual« May 14 - Aug 13
 2021 Week 20
  • May-17 Mo
O2k-Open SupportInfo: improve Planning to measure proton flux or mitochondrial membrane potential with ion-selective electrodes in the O2k?

Learn how to assemble the reference electrodes:
- »O2k-Videosupport: Assembly of the reference electrode«
- communicated by »Cardoso Luiza« and »Schmitt Sabine«

  • May-18 Tu

  • May-19 We
O2k-Publications in the MiPMap Antidiabetic drugs and blood cells – high-resolution respirometry shows PBMCs and platelets as a potential model to predict mitochondrial drug toxicity
from the O2k-Network »SE Lund Elmer E«
  • Piel S, Ehinger JK, Elmér E, Hansson Magnus J (2014) Metformin induces lactate production in peripheral blood mononuclear cells and platelets through specific mitochondrial Complex I inhibition. Acta Physiol (Oxf) 213:171-80.»Bioblast link«

  • May-20 Th
11:00-11:30 am (GMT+2): Schmitt Sabine and Went Nora at ISAP 2021 Virtual - Oroboros Instruments Q&A Session

  • May-21 Fr
 2021 Week 21
  • May-24 Mo
Immediately after switching off the light, a sharp peak of light-enhanced dark respiration is observed in algae.

- »Light-enhanced dark respiration«

  • May-25 Tu
O2k-Open SupportTake care Do you enter the correct instrumental background correction in DatLab for the experimental O2 concentrations?

After performing the instrumental background test at the appropriate O2 range, load the parameters in DatLab to ensure accurate correction for external fluxes.
- »O2 background correction in DatLab'
- communicated by »Komlodi Timea« and »Cecatto Cristiane«

  • May-26 We
»24th Kalorimetrietage 2021 Braunschweig DE« May 26-28

  • May-27 Th
»4th edition Metabolism & Cancer 2021 Virtual« May 27-29 - Visit the Oroboros booth
13:10-13:20 pm (GMT+2): Gnaiger Erich at 24th Kalorimetrietage 2021 Braunschweig DE - The three main features of the O2k

  • May-28 Fr
11:25 am (GMT+2): Gnaiger Erich presenting at 4th edition Metabolism & Cancer 2021 Virtual - Oroboros Instruments innovations - NextGen-O2k and Bioenergetics Communications

Apr 2021

  • Apr-1 Th
Abstract submission deadline for »SfRBM 2021 Virtual«

  • Apr-2 Fr - World Autism Awareness Day
O2k-brief repository Impaired mitochondrial function correlates to autism endophenotypes in mice
from the O2k-Network »DE Ulm Radermacher P« and »US PA Philadelphia Wallace DC«
  • Yardeni T, Cristancho AG, McCoy AJ, Schaefer PM, McManus MJ, Marsh ED, Wallace DC (2021) An mtDNA mutant mouse demonstrates that mitochondrial deficiency can result in autism endophenotypes. Proc Natl Acad Sci U S A 118:e2021429118. »Bioblast link«
 2021 Week 14
  • Apr-05 Mo

  • Apr-06 Tu
O2k-Open Support Warning: loss of data A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.

See how you can check that the OroboPOS-Holders are intact.
- »O2k-OroboPOS-Holder«

  • Apr-07 We

  • Apr-08 Th - Parkinson’s Disease Awareness Month
O2k-Publications in the MiPMap Dominant mutation in Parkinson’s Disease causes loss of protein function in mitochondria
from the O2k-Network »FR Angers Gueguen N« and »BE Leuven Spinazzi M«
  • Cornelissen T, Spinazzi M, Martin S, Imberechts D, Vangheluwe P, Bird M, De Strooper B, Vandenberghe W (2020) CHCHD2 harboring the Parkinson's disease-linked T61I mutation precipitates inside mitochondria and induces precipitation of wild-type CHCHD2. Hum Mol Genet [Epub ahead of print]. — »Bioblast link«

  • Apr-09 Fr

  • Apr-10 Sa

  • Apr-11 Su - World Parkinson's Disease Day
O2k-brief repository Parkinson model with 6-hydroxydopamine shows brain-region specific mitochondrial bioenergetics
from the O2k-Network »BR Santa Maria Soares FA«
  • Gonçalves DF, Courtes AA, Hartmann DD, da Rosa PC, Oliveira DM, Soares FAA, Dalla Corte CL (2018) 6-Hydroxydopamine induces different mitochondrial bioenergetics response in brain regions of rat. Neurotoxicology 70:1-11. — »Bioblast link«

2021 Week 15
  • Apr-12 Mo

  • Apr-13 Tu
O2k-Open SupportWarning: loss of data Do you know what to do when the fluorescence signal starts at a negative value?

A negative signal may be caused by wet Fluo-Sensors. For further information:
- »Negative fluorescence signal«
-communicated by »Komlodi Timea« and »Cecatto Cristiane«

  • Apr-14 We

  • Apr-15 Th
O2k-brief repository How running an ultramarathon affects platelet mitochondrial function
from the O2k-Network »IT Verona Calabria E«, »AT Innsbruck Burtscher M«, »DE Duesseldorf Roden M«, »AT Innsbruck Gnaiger E« and »AT Innsbruck Oroboros«
  • Hoppel F, Calabria E, Pesta DH, Kantner-Rumplmair W, Gnaiger E, Burtscher M (2021) Effects of ultramarathon running on mitochondrial function of platelets and oxidative stress parameters: a pilot study. Front Physiol 12:632664. »Bioblast link«

  • Apr-16 Fr
Abstract submission deadline for »24th Kalorimetrietage 2021 Braunschweig DE«

2021 Week 16
  • Apr-19 Mo

  • Apr-20 Tu
O2k-Open SupportWarning: loss of data Noisy fluorescence signal?

Follow these steps to localize and solve the problem:
- »Noisy fluorescence signal«
-communicated by »Komlodi Timea« and »Cecatto Cristiane«

  • Apr-21 We

  • Apr-22 Th
O2k-Publications in the MiPMap Succinate anaplerosis enhances the malignant potential of prostate cancer cells.
from the O2k-Network »AT Innsbruck Gnaiger E« and »FR Bordeaux Rossignol R«
  • Bastos Sant'Anna Silva AC, Perez-Valencia JA, Sciacovelli M, Lalou C, Sarlak S, Tronci L, Nikitopoulou E, Meszaros AT, Frezza C, Rossignol R, Gnaiger E, Klocker H (2021) Succinate anaplerosis has an onco-driving potential in prostate cancer cells. Cancers 13:1727.»Bioblast link«

  • Apr-23 Fr

2021 Week 17
  • Apr-26 Mo

  • Apr-27 Tu
O2k-Open SupportTake care Why is the fluorescence signal constantly at 10 V or at 0 V?

Follow these steps to localize and solve the problem:
- »Fluorescence signal is constantly at 10 V« and »0 V fluorescence signal«
- communicated by »Komlodi Timea« and »Cecatto Cristiane« 

  • Apr-28 We
O2k-Publications in the MiPMap pH-regulated succinate uptake in prostate cancer
from the O2k-Network »US PA Philadelphia Orynbayeva Z«
  • Zhunussova A, Sen B, Friedman L, Tuleukhanov S, Brooks AD, Sensenig R, Orynbayeva Z (2015) Tumor microenvironment promotes dicarboxylic acid carrier-mediated transport of succinate to fuel prostate cancer mitochondria. Am J Cancer Res 5:1665-79. »Bioblast link«

  • Apr-29 Th

  • Apr-30 Fr
Registration deadline for »24th Kalorimetrietage 2021 Braunschweig DE«

Mar 2021

2021 Week 9
  • Mar-1 Mo
O2k-Publications in the MiPMap Complex I of the mitochondrial electron transfer system is an important druggable target in Alzheimer's disease
from the O2k-Network »US NY New York Galkin A«
  • Stojakovic A, Trushin S, Sheu A, Khalili L, Chang SY, Li X, Christensen T, Salisbury JL, Geroux RE, Gateno B, Flannery PJ, Dehankar M, Funk CC, Wilkins J, Stepanova A, O'Hagan T, Galkin A, Nesbitt J, Zhu X, Tripathi U, Macura S, Tchkonia T, Pirtskhalava T, Kirkland JL, Kudgus RA, Schoon RA, Reid JM, Yamazaki Y, Kanekiyo T, Zhang S, Nemutlu E, Dzeja P, Jaspersen A, Kwon YIC, Lee MK, Trushina E (2021) Partial inhibition of mitochondrial complex I ameliorates Alzheimer's disease pathology and cognition in APP/PS1 female mice. Commun Biol 4:61. »Bioblast link«

  • Mar-2 Tu
Measuring fatty acid oxidation (FAO)-linked respiration requires a low concentration of malate

In the absence of malate, accumulation of Acetyl-CoA inhibits FAO.
- »Fatty acid oxidation pathway control state«, »Mitochondrial Pathways: Chapter 5« 
- communicated by »Cecatto Cristiane« and »Cardoso Luiza« 

  • Mar-3 We
FAT4BRAIN »FAT4BRAIN O2k-Workshop IOC148 Virtual Event« (Mar 3-4)

  • Mar-4 Th

  • Mar-05 Fr

 2021 Week 10
  • Mar-08 Mo
Abstract submission deadline for: »ESCI 2021 Virtual«

  • Mar-10 We
MitoFit pdf Mitochondrial physiology in Parkinson’s disease (PD)

A COST Action MitoEAGLE contribution:

  • Krako Jakovljevic N, Ebanks B, Chakrabarti L, Markovic I, Moisoi N (2021) Mitochondrial homeostasis in cellular models of Parkinson’s Disease. MitoFit Preprints 2021.4 doi:10.26124/mitofit:2021-0004

  • Mar-11 Th
EU-CARDIOPROTECTION.png »6th EU-Cardioprotection Meeting 2021 Virtual«
Gnaiger 2020 BEC MitoPathways Internal normalization of rate – what is the difference between flux control ratio and flux control efficiency?

A flux control ratio is the ratio of oxygen flux in a specific respiratory control state, normalized for maximum flux in a common reference state. Flux control efficiency expresses the control of respiration as a fractional change of oxygen flux or flow between protocol steps. Both values vary between theoretical lower and upper limits of 0 and 1.
- »Gnaiger 2020 BEC MitoPathways - Chapter 3« 
- » Posted by: »Komlodi Timea« and »Cecatto Cristiane«

  • Mar-12 Fr
2021 Week 11
  • Mar-15 Mo

  • Mar-16 Tu
CI inhibitors like rotenone inhibit fatty acid oxidation (FAO)-linked respiration

Inhibition of CI leads to accumulation of NADH in the mt-matrix. The resultant lack of NAD+ blocks FAO.
- »Fatty acid oxidation pathway control state«, »Mitochondrial Pathways: Chapter 5« 
- communicated by »Cardoso Luiza« and »Cecatto Cristiane«

  • Mar-17 We

  • Mar-18 Th
O2k-Publications in the MiPMap Mitochondrial function in platelets of ultramarathon runners
from the O2k-Network »IT Verona Calabria E«, »AT Innsbruck Burtscher M«, »DE Duesseldorf Roden M«, »AT Innsbruck Gnaiger E« and »AT Innsbruck Oroboros«
  • Hoppel F, Calabria E, Pesta DH, Kantner-Rumplmair W, Gnaiger E, Burtscher M (2021) Effects of ultramarathon running on mitochondrial function of platelets and oxidative stress parameters: a pilot study. Front Physiol 12:632664. »Bioblast link«

  • Mar-19 Fr
2021 Week 12 
  • Mar-22 Mo
TRANSMIT Acronym.jpg»TRANSMIT School 2021 Virtual« Mar 22-23

  • Mar-24 We - World Tuberculosis Day
O2k-Publications in the MiPMap The synthetic peptide IP-1 as a treatment for multidrug-resistant tuberculosis
from the O2k-Network »MX Mexico City Uribe-Carvajal S«
  • Peláez Coyotl EA, Barrios Palacios J, Muciño G, Moreno-Blas D, Costas M, Montiel Montes T, Diener C, Uribe-Carvajal S, Massieu L, Castro-Obregón S, Espinosa OR, Mata Espinosa D, Barrios-Payan J, León Contreras JC, Corzo G, Hernández-Pando R, Del Rio G (2020) Antimicrobial peptide against Mycobacterium tuberculosis that activates autophagy is an effective treatment for tuberculosis. Pharmaceutics 12:E1071. »Bioblast link«

  • Mar-25 Th
O2k-Open SupportInfo: improve O2k-checklist: Get started with an O2k-experiment

The O2k-checklist provides an overview of all essential steps for starting an O2k-experiment.
- » MiPNet20.04_O2k-checklist« 
- » communicated by »Cecatto Cristiane« and »Schmitt Sabine«

  • Mar-26 Fr
MitoEAGLE dissemination - did you receive your package?

Bioenergetics Communications - to enhance reproducibility in mitochondrial physiology
- including: the »MitoEAGLE Poster« and »Mitochondrial pathways and respiratory control«

2021 Week 13

  • Mar-29 Mo
MitoEAGLE dissemination - did you receive your package?

Bioenergetics Communications - to enhance reproducibility in mitochondrial physiology
- including: the »MitoEAGLE Poster« and »Mitochondrial pathways and respiratory control«

  • Mar-30 Tu

  • Mar-31 We
O2k-Publications in the MiPMap Diabetes, infarct size, mitochondrial respiration and succinate dehydrogenase inhibition - how do they relate?
from the O2k-Network »DK Aarhus Boetker HE«
  • Tonnesen PT, Hjortbak MV, Lassen TR, Seefeldt JM, Bøtker HE, Jespersen NR (2021) Myocardial salvage by succinate dehydrogenase inhibition in ischemia-reperfusion injury depends on diabetes stage in rats. Mol Cell Biochem [Epub ahead of print].»Bioblast link«

Feb 2021

 2021 Week 5
  • Feb-01 Mo

  • Feb-02 Tu
O2k-Open SupportInfo: improve DatLab error messages: What do they mean and how to solve them?

- » DatLab error messages« 
- communicated by »Garcia-Souza Luiz F« and »Tindle-Solomon Lisa«

  • Feb-03 We

  • Feb-04 Th
O2k-Publications in the MiPMap Could inhibitors of Dihydroorotate-dehydrogenase be anti-cancer agents?
from the O2k-Network »CZ Prague Neuzil J« »AU Queensland Neuzil J« »NL Nijmegen Koopman WJ« »AU Sydney Stocker R« »AU Queensland Peart J«
  • Bajzikova M, Kovarova J, Coelho AR, Boukalova S, Oh S, Rohlenova K, Svec D, Hubackova S, Endaya B, Judasova K, Bezawork-Geleta A, Kluckova K, Chatre L, Zobalova R, Novakova A, Vanova K, Ezrova Z, Maghzal GJ, Magalhaes Novais S, Olsinova M, Krobova L, An YJ, Davidova E, Nahacka Z, Sobol M, Cunha-Oliveira T, Sandoval-Acuña C, Strnad H, Zhang T, Huynh T, Serafim TL, Hozak P, Sardao VA, Koopman Werner JH, Ricchetti M, Oliveira PJ, Kolar F, Kubista M, Truksa J, Dvorakova-Hortova K, Pacak K, Gurlich R, Stocker R, Zhou Y, Berridge MV, Park S, Dong L, Rohlena J, Neuzil J (2019) Reactivation of dihydroorotate dehydrogenase-driven pyrimidine biosynthesis restores tumor growth of respiration-deficient cancer cells. Cell Metab 29:399-416. »Bioblast link«

  • Feb-05 Fr

 2021 Week 6
  • Feb-08 Mo
O2k-Open SupportInfo: improve Do you know how to properly insert the ISS-tip into the O2k-chamber?

With the stirrer bar still rotating, insert the ISS-tip to the bottom of the O2k-Chamber, so that it points away from you.
- »MiPNet19.03 O2k-cleaning and ISS: Section 2« 
- communicated by »Doerrier Carolina«, »Cecatto Cristiane« and »Komlodi Timea«

  • Feb-10 We
O2k-Publications in the MiPMap Approved FAO inhibitor Trimetazidine restricts tumor growth in oxidative lung carcinomas
from the O2k-Network »FR Bordeaux Rossignol R«
  • Amoedo ND, Sarlak S, Obre E, Esteves P, Bégueret H, Kieffer Y, Rousseau B, Dupis A, Izotte J, Bellance N, Dard L, Redonnet-Vernhet I, Punzi G, Rodrigues MF, Dumon E, Mafhouf W, Guyonnet-Dupérat V, Gales L, Palama T, Bellvert F, Dugot-Senan N, Claverol S, Baste JM, Lacombe D, Rezvani HR, Pierri CL, Mechta-Grigoriou F, Thumerel M, Rossignol R (2021) Targeting the mitochondrial trifunctional protein restrains tumor growth in oxidative lung carcinomas. J Clin Invest 131:e133081. »Bioblast link«

  • Feb-11 Th

  • Feb-12 Fr

  • Feb-13 Sa

  • Feb-14 Su Donor Day
O2k-Publications in the MiPMap Preservation solutions are superior to cardioplegic solutions for prolonged cold storage of the heart
from the O2k-Network »AT Innsbruck Gnaiger E« and »AT Innsbruck Oroboros«
  • Schneeberger S, Kuznetsov AV, Seiler R, Renz O, Meusburger H, Mark W, Brandacher G, Margreiter R, Gnaiger E (2008) Mitochondrial ischemia-reperfusion injury of the transplanted rat heart: improved protection by preservation versus cardioplegic solutions. Shock 30:365-71. »Bioblast link«
 2021 Week 7
  • Feb-15 Mo International Childhood Cancer Day
O2k-Publications in the MiPMap Glucocorticoids induce apoptosis via repressed mitochondrial respiration and altered expression of mt-transporters in childhood acute lymphoblastic leukemia
from the O2k-Network »DE Regensburg Renner-Sattler K« and »AT Innsbruck Gnaiger E«
  • Eberhart K, Rainer J, Bindreither D, Ritter I, Gnaiger E, Kofler R, Oefner PJ, Renner K (2011) Glucocorticoid-induced alterations in mitochondrial membrane properties and respiration in childhood acute lymphoblastic leukemia. Biochim Biophys Acta 1807:719-25. »Bioblast link«

  • Feb-16 Tu

  • Feb-17 We
O2k-Open SupportInfo: improve Improve preparation of permeabilized muscle fibers

The application of high-resolution respirometry in permeabilized muscle fibers provides a sensitive diagnostic tool for the analysis of mitochondrial (dys)function in small biopsy samples.
- »O2k-Videosupport: Fiber permeabilization« 
- »Doerrier 2018 Methods Mol Biol« 
- communicated by »Doerrier Carolina« and »Tindle-Solomon Lisa«

  • Feb-18 Th

  • Feb-19 Fr
 2021 Week 8
  • Feb-22 Mo
MitoFit pdf Exploring the Q-junction in real-time

Simultaneous measurement of coenzyme Q redox state and respiration in isolated mitochondria:

  • Komlodi T, Cardoso LHD, Doerrier C, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. MitoFit Preprints 2021.2. doi:10.26124/mitofit:2021-0002

  • Feb-24 We
O2k-Publications in the MiPMap Anticancer effect of metformin in myeloproliferative neoplasms: downregulation of JAK2/STAT signaling and mitochondrial activity
from the O2k-Network »BR Ribeirao Preto Alberici LC«
  • Machado-Neto JA, Fenerich BA, Scopim-Ribeiro R, Eide CA, Coelho-Silva JL, Dechandt CRP, Fernandes JC, Rodrigues Alves APN, Scheucher PS, Simões BP, Alberici LC, de Figueiredo Pontes LL, Tognon CE, Druker BJ, Rego EM, Traina F (2018) Metformin exerts multitarget antileukemia activity in JAK2V617F-positive myeloproliferative neoplasms. Cell Death Dis 9:311. »Bioblast link«

  • Feb-25 Th
MitoFit pdf Diagnosis of mitochondrial disease in frozen samples

Measurement of respiratory function in cryopreserved muscle biopsies:
Zuccolotto-dos-Reis FH, Andriao-Escarso SH, Araujo JS, Espreafico EM, Alberici LC, Sobreira CFR (2021) Acetyl CoA driven respiration in frozen muscle contributes to the diagnosis of mitochondrial disease. MitoFit Preprints 2021.3. doi:10.26124/mitofit:2021-0003

  • Feb-26 Fr

  • Feb-27 Sa

  • Feb-28 Su Rare disease day
O2k-Publications in the MiPMap Wolframin deficiency-related changes in the bioenergetics of mouse muscle
from the O2k-Network »EE Tallinn Kaambre T« »EE Tartu Paju K«
  • Tepp K, Puurand M, Timohhina N, Aid-Vanakova J, Reile I, Shevchuk I, Chekulayev V, Eimre M, Peet N, Kadaja L, Paju K, Kaambre T (2020) Adaptation of striated muscles to Wolframin deficiency in mice: Alterations in cellular bioenergetics. Biochim Biophys Acta Gen Subj 1864:129523. »Bioblast link«

Jan 2021

  • Jan-01 Fr
Happy New Year!
 2021 Week 1
  • Jan-04 Mo
The Oroboros office is enjoying a break from December 24th to January 6th.

  • Jan-05 Tu

  • Jan-06 We

  • Jan-07 Th
Oroboros Instruments is pleased to be a collaborator on the CureMILS project that has recently been approved for EJP-RD funding.

Led by O2k-Network member, Alessandro Prigione, the project aims to employ a novel approach to enable drug discovery of Mitochondrial DNA (mtDNA)-associated Leigh syndrome (MILS).

- Read more »CureMILS«

  • Jan-08 Fr
O2k-brief repository Liver mitochondrial adaptation in a mouse model of NAFLD induced by a Western-style diet
from the O2k-Network »CZ Hradec Kralove Cervinkova Z«
  • Staňková P, Kučera O, Peterová E, Lotková H, Maseko TE, Nožičková K, Červinková Z (2020) Adaptation of mitochondrial substrate flux in a mouse model of nonalcoholic fatty liver disease. Int J Mol Sci 21:E1101. »Bioblast link«

2021 Week 2
  • Jan-11 Mo
O2k-Open SupportInfo: improve Are you a beginner or need a refresher on using SUIT protocols with DatLab7.4?

- » O2k-Videosupport: How to perform an experiment with a SUIT DL-Protocol« and » Run DL-Protocol/Set O2 limit in DatLab « 
- communicated by »Komlodi Timea« and Schmitt Sabine«

  • Jan-13 We
O2k-Publications in the MiPMap Positive effect of regular physical activity on mitochondrial respiration in burnout syndrome
from the O2k-Network »CH Basel Eckert A«
  • Brand S, Ebner K, Mikoteit T, Lejri I, Gerber M, Beck J, Holsboer-Trachsler E, Eckert A (2020) Influence of regular physical activity on mitochondrial activity and symptoms of burnout-an interventional pilot study. J Clin Med 9:E667.»Bioblast link«

  • Jan-14 Th

  • Jan-15 Fr
2021 Week 3
  • Jan-18 Mo

  • Jan-19 Tu
O2k-Publications in the MiPMap Complex III is essential in mitochondrial electron transfer system maturation and supercomplex formation
from the O2k-Network »US FL Miami Moraes CT«
  • Protasoni M, Pérez-Pérez R, Lobo-Jarne T, Harbour ME, Ding S, Peñas A, Diaz F, Moraes CT, Fearnley IM, Zeviani M, Ugalde C, Fernández-Vizarra E (2020) Respiratory supercomplexes act as a platform for complex III-mediated maturation of human mitochondrial complexes I and IV. EMBO J 39:e102817. »Bioblast link«

  • Jan-20 We
Gnaiger 2020 BEC MitoPathways A quote from MitoPathways Chapter 3. Normalization:

Respiratory OXPHOS capacity P in human skeletal muscle ranges from 60 to 160 pmol∙s­-1∙mg­-1 based on wet muscle mass of permeabilized fibers. By comparison, mass-specific electron transfer capacity E is approximately 25 pmol∙s-1∙mg-1 in platelets and senescent fibroblasts and 40 to 80 pmol∙s-1∙mg-1 in PBMC, HEK, CEM, HUVEC, and young fibroblasts. ― Are these values comparable? - »Gnaiger 2020 BEC MitoPathways«.

  • Jan-21 Th
Measuring ATP production with Magnesium Green – which sample preparations work?

Magnesium Green (MgG) can be used with mitochondrial preparations: isolated mitochondria, tissue homogenates, and permeabilized cells and tissues.
- »MgG« and »Publications MgG« 
- communicated by »Cardoso Luiza« and »Komlodi Timea«

  • Jan-22 Fr
2021 Week 4
  • Jan-25 Mo

  • Jan-27 We
O2k-Publications in the MiPMap Cell-permeable succinate prodrug NV118 improves statin-induced mitochondrial dysfunction in human platelets
from the O2k-Network »RO Timisoara Muntean DM« and »SE Lund Elmer E«
  • Avram VF, Chamkha I, Åsander-Frostner E, Ehinger JK, Timar RZ, Hansson MJ, Muntean DM, Elmér E (2021) Cell-permeable succinate rescues mitochondrial respiration in cellular models of statin toxicity. Int J Mol Sci 22:424. »Bioblast link«

  • Jan-28 Th
Gnaiger 2020 BEC MitoPathways A quote from MitoPathways Chapter 6. Convergent electron transfer at the Q-junction:

The concept on convergent electron transfer at the Q-junction (ETS) challenges conventional OXPHOS analysis based on the ETC terminology and a way of thinking about the mitochondrial ‘electron transfer chain’ - »Gnaiger 2020 BEC MitoPathways«.

  • Jan-29 Fr
MitoFit pdf SUIT protocols with Magnesium Green™ (MgG) can be used to study respiration and ATP production P»

MgG dye does not affect respiration of cardiac isolated mitochondria.

  • Cardoso LHD, Doerrier C, Gnaiger E (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. MitoFit Preprints 2021.1. doi:10.26124/mitofit:2021-0001.

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