MitoPediaAlert2020 Archive: Difference between revisions
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| '''Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?''' <br> | | '''Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?''' <br> | ||
- »'''[[Setting_the_oxygen_concentration |Setting the oxygen concentration]]'''« | - »'''[[Setting_the_oxygen_concentration |Setting the oxygen concentration]]'''« | ||
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| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]] | |||
| '''Do you know how to select the proper Fluorescence-Sensor and filter set?''' <br> | |||
Using different Fluorescence-Sensors and [[Filters]], the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] allows for measurement of [[Hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential]], [[Calcium]], and numerous other applications open for O2k-user innovation.<br> | |||
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors | Selecting the Smart Fluo-Sensor]]'''« and »'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module | Setup of the O2k-Fluo LED2-Module]]'''« | |||
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Revision as of 08:11, 13 July 2020
- Currently Answered Questions from the »Oroboros Ecosystem Agenda«
Jul 2020
- Jul-02 Th
Did you know that if ET capacity is lower than OXPHOS capacity
(P>E) in living cells or mitochondrial preparations, then you have encountered an experimental artefact? The possible triggers of this experimental artefact are:
- loss of oxidative capacity during the time course of the respirometric assay,
- insufficient uncoupler concentrations,
- over titration of an uncoupler, which inhibits ET capacity,
- inhibition of ET capacity due to the use of high oligomycin (inhibitor of the ATP synthase) concentrations employed to measure LEAK respiration prior ET capacity. Information from: Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »Bioblast link«
- Jul-09 Th
Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?
- Jul-16 Th
Do you know how to select the proper Fluorescence-Sensor and filter set?
Using different Fluorescence-Sensors and Filters, the O2k-Fluo Smart-Module allows for measurement of Hydrogen peroxide, ATP, Mitochondrial membrane potential, Calcium, and numerous other applications open for O2k-user innovation.
- » Selecting the Smart Fluo-Sensor« and » Setup of the O2k-Fluo LED2-Module«