Difference between revisions of "Respiratory acceptor control ratio"
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|abbr=''RCR'' | |abbr=''RCR'' | ||
|description=The '''respiratory acceptor control ratio''' (''RCR'') is defined as [[State 3]]/[[State 4]] [1]. If State 3 is measured at saturating [ADP], ''RCR'' is the inverse of the OXPHOS control ratio, ''[[L/P]]'' (when State 3 is equivalent to the OXPHOS state, ''P''). ''RCR'' is directly but non-linearly related to the [[OXPHOS coupling efficiency]], ''j<sub>≈P</sub>'' = 1-''L/P''. Whereas the normalized flux ratio ''j<sub>≈P</sub>'' has boundaries from 0.0 to 1.0, ''RCR'' ranges from 1.0 to infinity, which needs to be considered when performing statistical analyses. In intact cells, the term ''RCR'' has been used for the ratio [[State 3u]]/[[State 4o]], i.e. for the inverse ''[[L/E]]'' ratio [2,3]. Then for conceptual and statistical reasons, ''RCR'' should be replaced by the [[ET-coupling efficiency]], ''j<sub>≈E</sub>''= 1-''L/E'' [4]. | |description=The '''respiratory acceptor control ratio''' (''RCR'') is defined as [[State 3]]/[[State 4]] [1]. If State 3 is measured at saturating [ADP], ''RCR'' is the inverse of the OXPHOS control ratio, ''[[L/P]]'' (when State 3 is equivalent to the OXPHOS state, ''P''). ''RCR'' is directly but non-linearly related to the [[OXPHOS coupling efficiency]], ''j<sub>≈P</sub>'' = 1-''L/P''. Whereas the normalized flux ratio ''j<sub>≈P</sub>'' has boundaries from 0.0 to 1.0, ''RCR'' ranges from 1.0 to infinity, which needs to be considered when performing statistical analyses. In intact cells, the term ''RCR'' has been used for the ratio [[State 3u]]/[[State 4o]], i.e. for the inverse ''[[L/E]]'' ratio [2,3]. Then for conceptual and statistical reasons, ''RCR'' should be replaced by the [[ET-coupling efficiency]], ''j<sub>≈E</sub>''= 1-''L/E'' [4]. | ||
− | |info=[[Chance 1955 J Biol Chem-I]], [[Gnaiger | + | |info=[[Chance 1955 J Biol Chem-I]], [[Gnaiger 2020 MitoPathways]], [[BEC 2020.1]] |
}} | }} | ||
== Compare == | == Compare == |
Latest revision as of 12:08, 23 May 2020
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Respiratory acceptor control ratio
Description
The respiratory acceptor control ratio (RCR) is defined as State 3/State 4 [1]. If State 3 is measured at saturating [ADP], RCR is the inverse of the OXPHOS control ratio, L/P (when State 3 is equivalent to the OXPHOS state, P). RCR is directly but non-linearly related to the OXPHOS coupling efficiency, j_{≈P} = 1-L/P. Whereas the normalized flux ratio j_{≈P} has boundaries from 0.0 to 1.0, RCR ranges from 1.0 to infinity, which needs to be considered when performing statistical analyses. In intact cells, the term RCR has been used for the ratio State 3u/State 4o, i.e. for the inverse L/E ratio [2,3]. Then for conceptual and statistical reasons, RCR should be replaced by the ET-coupling efficiency, j_{≈E}= 1-L/E [4].
Abbreviation: RCR
Reference: Chance 1955 J Biol Chem-I, Gnaiger 2020 MitoPathways, BEC 2020.1
Compare
- OXPHOS coupling efficiency
- Discussion: OXPHOS versus ET-capacity.
- More details: »ET-coupling efficiency
- Discussion
- Bioblast links: Uncoupling - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Specific
- » Artefacts by single dose uncoupling
- » ATP synthase
- » CCCP
- » Coupling control protocol
- » DNP
- » Dyscoupled respiration
- » FCCP
- » Gnaiger 2019 MitoFit Preprint Arch
- » Is respiration uncoupled - noncoupled - dyscoupled?
- » Noncoupled respiration: Discussion
- » Uncoupler
- » Uncoupled respiration - see » Noncoupled respiration
- » Uncoupling proteins
- » Uncoupling protein 1
- » Uncoupler titrations - Optimum uncoupler concentration
- Specific
- Respiratory states and control ratios
- » Biochemical coupling efficiency
- » Coupling control state
- » Electron transfer-pathway state
- » Electron transfer-pathway
- ET-capacity
- » ET-coupling efficiency
- » Flux control factor
- » Flux control ratio
- » LEAK control ratio
- » LEAK respiration
- » Noncoupled respiration
- » OXPHOS
- » OXPHOS capacity; » State 3
- » OXPHOS control ratio, P/E ratio
- » Respiratory acceptor control ratio
- » ROUTINE control ratio
- » ROUTINE-respiration
- » ROUTINE state
- » State 3u
- » State 4
- » Uncoupler control ratio, UCR
- Respiratory states and control ratios
- General (alphabetical order)
- Other keyword lists
References
- Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation. I. Kinetics of oxygen utilization. J Biol Chem 217:383-93. - »Bioblast link« - "No extremely rapid phase of respiration is observed to precede the steady rate caused by the addition of phosphate acceptor; phosphate acceptor sets the respiratory system into operation very rapidly and at a relatively constant rate. The constant-respiration rate of the phosphorylating system is maintained until the concentration of phosphate acceptor has fallen to a low value; then the respiration abruptly falls by a factor of 4- to l0-fold, depending upon the quality of the preparation. This abrupt decrease of respiration is due to the high affinity of the oxidative phosphorylation system for ADP."
- Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28. - »Bioblast link«
- Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41:1837–45. - »Bioblast link«
- Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 17.18. OROBOROS MiPNet Publications, Innsbruck. - »Bioblast link«
MitoPedia concepts:
Respiratory control ratio
MitoPedia methods:
Respirometry