SF6847

high-resolution terminology - matching measurements at high-resolution

SF6847

Description

SF6847 (C₁₈H₂₂N_2O), also known as tyrphostin A9 or malonoben, is a protonophore and a very potent uncoupler of oxidative phosphorylation, being used in the nM range. Like all uncouplers, SF6847 concentrations must be titrated carefully to evaluate the optimum concentration for maximum stimulation of mitochondrial respiration, particularly to avoid inhibition of respiration at higher concentrations.

Abbreviation: SF6847

Reference: Chinopoulos 2014 Methods Enzymol, Terada 1984 Biochim Biophys Acta

Application in HRR

Preparation

SF 6847/tyrphostin A9/malonoben (C₁₈H₂₂N_2O, [[3,5-bis(1,1-Dimethylethyl)-4-hydroxyphenyl]methylene]propanedinitrile; Sigma T182, 5 mg: M = 282.38 g·mol^-1. Alternative source: Enzo, BML-EI215.

Caution: Toxic!

Preparation of 50 mM stock solution (dissolved in 99.9% ethanol):

1. Weigh 5 mg in a dark glass vial: dissolve with 354.1 µL ethanol.
2. Store in glass vials at -20 °C.

Preparation of 500 µM stock solution (dissolved in 99.9% ethanol):

1. Add 990 µL ethanol and 10 µL of the 50 mM stock solution to a dark glass vial.
2. Store in glass vials at -20 °C.

Preparation of 50 µM stock solution (dissolved in 99.9% ethanol):

1. Add 900 µL ethanol and 100 µL of the 500 µM stock solution to a dark glass vial.
2. Divide into 0.1 ml aliquotes, in dark glass vials.
3. Store at -20 °C.

O2k manual titrations MiPNet09.12 O2k-Titrations

• Titration volume: 1 µl steps 50 µM stock solution using a 10 µL syringe (2 ml O2k-Chamber).
• Final concentration: 25 nM steps.

Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.

Bioblast links: Uncoupling - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

Specific

» Artefacts by single dose uncoupling

» ATP synthase

» CCCP

» Coupling-control protocol

» DNP

» Dyscoupled respiration

» FCCP

» Is respiration uncoupled - noncoupled - dyscoupled?

» Noncoupled respiration: Discussion

» Uncoupler

» Uncoupled respiration - see » Noncoupled respiration

» Uncoupling proteins

» Uncoupling protein 1

» Uncoupler titrations - Optimum uncoupler concentration

Respiratory states and control ratios

» Biochemical coupling efficiency

» Coupling-control state

» Electron-transfer-pathway state

» Electron-transfer pathway

ET capacity

» E-L coupling efficiency

» Flux control efficiency

» Flux control ratio

» LEAK-control ratio

» LEAK respiration

» Noncoupled respiration

» OXPHOS

» OXPHOS capacity; » State 3

» OXPHOS-control ratio, P/E ratio

» Respiratory acceptor control ratio

» ROUTINE-control ratio

» ROUTINE respiration

» ROUTINE state

» State 3u

» State 4

» Uncoupling-control ratio UCR

• Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1
• Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002

General (alphabetical order)

» Adenine nucleotide translocase

» Adenylates

» Electron transfer pathway

» Mitochondrial preparations

» mt-membrane potential

» Oxygen flux

» Phosphorylation system

» Proton leak

» Proton slip

» TIP2k

Other keyword lists

» Template:Keywords: Force and membrane potential

MitoPedia topics: Uncoupler