Bioblast alert - 2021
Bioblast alert 2021(04) - MitoFit Preprints: 2021-02-19
||Exploring the Q-junction in real-time|
Simultaneous measurement of coenzyme Q redox state and respiration in isolated mitochondria:
- Komlodi T, Cardoso LHD, Doerrier C, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. MitoFit Preprints 2021.2. doi:10.26124/mitofit:2021-0002
||Diagnosis of mitochondrial disease in frozen samples |
Measurement of respiratory function in cryopreserved muscle biopsies:
Zuccolotto-dos-Reis FH, Andriao-Escarso SH, Araujo JS, Espreafico EM, Alberici LC, Sobreira CFR (2021) Acetyl CoA driven respiration in frozen muscle contributes to the diagnosis of mitochondrial disease. MitoFit Preprints 2021.3. doi:10.26124/mitofit:2021-0003
Bioblast alert 2021(03) - ATP production P»: 2021-01-29
||SUIT protocols with Magnesium Green™ (MgG) can be used to study respiration and ATP production P» |
MgG dye does not affect respiration of cardiac isolated mitochondria.
- Cardoso LHD, Doerrier C, Gnaiger E (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. MitoFit Preprints 2021.1. doi:10.26124/mitofit:2021-0001
||Measuring ATP-ADP exchange using the fluorophore Magnesium Green |
- from the O2k-Network »HU Budapest Chinopoulos C«
- Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. »Bioblast link«
||Magnesium Green and P» – which sample preparations work?|
Magnesium Green (MgG) can be used with mitochondrial preparations: isolated mitochondria, tissue homogenates, and permeabilized cells and tissues.
- »MgG« and »Publications MgG«
- communicated by »Cardoso Luiza« and »Komlodi Timea«
Bioblast alert 2021(02) - Permeabilized fibers: 2021-01-21
||A quote from MitoPathways Chapter 3. Normalization:
Respiratory OXPHOS capacity P in human skeletal muscle ranges from 60 to 160 pmol∙s-1∙mg-1 based on wet muscle mass of permeabilized fibers. By comparison, mass-specific electron transfer capacity E is approximately 25 pmol∙s-1∙mg-1 in platelets and senescent fibroblasts and 40 to 80 pmol∙s-1∙mg-1 in PBMC, HEK, CEM, HUVEC, and young fibroblasts. ― Are these values comparable? - »Gnaiger 2020 BEC MitoPathways«.
||Statistical evaluation on replica in high-resolution respirometry with permeabilized skeletal muscle fibers |
- from the O2k-Network »AU Melbourne Bishop DJ«
- Jacques M, Kuang J, Bishop DJ, Yan X, Alvarez-Romero J, Munson F, Garnham A, Papadimitriou I, Voisin S, Eynon N (2019) Mitochondrial respiration variability and simulations in human skeletal muscle: The Gene SMART study. FASEB J 34:2978-86. - »Bioblast link«
|| Effect of temperature on mitochondrial respiratory capacity of cardiac permeabilized fibers|
- from the O2k-Network »AT Innsbruck Gnaiger E« , »CA Rimouski Blier PU« and »CA Edmonton Lemieux H«
- Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. »Bioblast link«
||What is the optimal ADP concentration to evaluate OXPHOS capacity in permeabilized muscle fibers (pfi)?|
For the evaluation of OXPHOS capacity, the limitation of respiratory fluxes must be avoided by using a kinetically-saturating ADP concentration. In permeabilized muscle fibers, an ADP concentration of 5 mM or higher is needed to reach kinetically-saturating levels.
- »Optimum ADP concentration in pfi«
Bioblast alert 2021(01) - MitoPathways: the new BEC edition of the Blue Book: 2021-01-05
||The 5th edition of MitoPathways features new chapters on additivity of mitochondrial pathway capacities and protonmotive pressure – one step beyond ∆Ψ and the protonmotive force.
Bioblast alert - previous issues