Bioblast alert 2021

Bioblast alert - 2021

Measurement of respiratory function in cryopreserved muscle biopsies:
Zuccolotto-dos-Reis FH, Andriao-Escarso SH, Araujo JS, Espreafico EM, Alberici LC, Sobreira CFR (2021) Acetyl CoA driven respiration in frozen muscle contributes to the diagnosis of mitochondrial disease. MitoFit Preprints 2021.3. doi:10.26124/mitofit:2021-0003

Bioblast alert 2021(03) - ATP production P»: 2021-01-29

SUIT protocols with Magnesium Green™ (MgG) can be used to study respiration and ATP production P»

MgG dye does not affect respiration of cardiac isolated mitochondria.

Cardoso LHD, Doerrier C, Gnaiger E (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. MitoFit Preprints 2021.1. doi:10.26124/mitofit:2021-0001

Measuring ATP-ADP exchange using the fluorophore Magnesium Green

from the O2k-Network »HU Budapest Chinopoulos C«

Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. »Bioblast link«

Magnesium Green and P» – which sample preparations work?

Magnesium Green (MgG) can be used with mitochondrial preparations: isolated mitochondria, tissue homogenates, and permeabilized cells and tissues.
- »MgG« and »Publications MgG«
- communicated by »Cardoso Luiza« and »Komlodi Timea«

Did you know that you can measure the respiratory capacity available for P» in living cells?

SUIT protocols from the SUIT-003 family guide you through the titrations to analyze R-L net ROUTINE capacity.
- »SUIT-003 O2 ce D009« and »SUIT-003 O2 ce-pce D020«
- communicated by »Cardoso Luiza« and »Komlodi Timea«

Bioblast alert 2021(02) - Permeabilized fibers: 2021-01-21

A quote from MitoPathways Chapter 3. Normalization:

Respiratory OXPHOS capacity P in human skeletal muscle ranges from 60 to 160 pmol∙s^-1∙mg^-1 based on wet muscle mass of permeabilized fibers. By comparison, mass-specific electron transfer capacity E is approximately 25 pmol∙s^-1∙mg^-1 in platelets and senescent fibroblasts and 40 to 80 pmol∙s^-1∙mg^-1 in PBMC, HEK, CEM, HUVEC, and young fibroblasts. ― Are these values comparable? - »Gnaiger 2020 BEC MitoPathways«.

Statistical evaluation on replica in high-resolution respirometry with permeabilized skeletal muscle fibers

from the O2k-Network »AU Melbourne Bishop DJ«

Jacques M, Kuang J, Bishop DJ, Yan X, Alvarez-Romero J, Munson F, Garnham A, Papadimitriou I, Voisin S, Eynon N (2019) Mitochondrial respiration variability and simulations in human skeletal muscle: The Gene SMART study. FASEB J 34:2978-86. - »Bioblast link«

Effect of temperature on mitochondrial respiratory capacity of cardiac permeabilized fibers

from the O2k-Network »AT Innsbruck Gnaiger E« , »CA Rimouski Blier PU« and »CA Edmonton Lemieux H«

Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. »Bioblast link«

What is the optimal ADP concentration to evaluate OXPHOS capacity in permeabilized muscle fibers (pfi)?

For the evaluation of OXPHOS capacity, the limitation of respiratory fluxes must be avoided by using a kinetically-saturating ADP concentration. In permeabilized muscle fibers, an ADP concentration of 5 mM or higher is needed to reach kinetically-saturating levels.
- »Optimum ADP concentration in pfi«

Bioblast alert 2021(01) - MitoPathways: the new BEC edition of the Blue Book: 2021-01-05

The 5^th edition of MitoPathways features new chapters on additivity of mitochondrial pathway capacities and protonmotive pressure – one step beyond ∆Ψ and the protonmotive force.

Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2: 112pp. doi:10.26124/bec:2020-0002. - »Open Access«