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Bioblast alert - 2021
Bioblast alert 2021(08) - Cell-permeable succinate: 2021-05-07
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A cell-permeable succinate prodrug can alleviate N-methyl carbamate-induced Complex I dysfunction
- from the O2k-Network »US PA Philadelphia Kilbaugh T«, »US PA Philadelphia Jang DH« and »SE Lund Elmer E«
- Janowska JI, Piel S, Saliba N, Kim CD, Jang DH, Karlsson M, Kilbaugh TJ, Ehinger JK (2020) Mitochondrial respiratory chain Complex I dysfunction induced by N-methyl carbamate ex vivo can be alleviated with a cell-permeable succinate prodrug carbamate toxicity and treatment. Toxicol In Vitro 65:104794. - »Bioblast link«
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Cell-permeable succinate can restore statin-inhibited mitochondrial respiration
- from the O2k-Network »RO Timisoara Muntean DM« and »SE Lund Elmer E«
- Avram VF, Chamkha I, Åsander-Frostner E, Ehinger JK, Timar RZ, Hansson MJ, Muntean DM, Elmér E (2021) Cell-permeable succinate rescues mitochondrial respiration in cellular models of statin toxicity. Int J Mol Sci 22:424. - »Bioblast link«
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Bioblast alert 2021(07) - Diabetes: 2021-04-15
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Diabetes, infarct size, mitochondrial respiration and succinate dehydrogenase inhibition - how do they relate?
- from the O2k-Network »DK Aarhus Boetker HE«
- Tonnesen PT, Hjortbak MV, Lassen TR, Seefeldt JM, Bøtker HE, Jespersen NR (2021) Myocardial salvage by succinate dehydrogenase inhibition in ischemia-reperfusion injury depends on diabetes stage in rats. Mol Cell Biochem [Epub ahead of print]. - »Bioblast link«
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Gastric bypass surgery improves diabetic nephropathy in type 1 diabetic rats
- from the O2k-Network »CN Chongqing Zhu Z«
- Wei X, Lu Z, Li L, Zhang H, Sun F, Ma H, Wang L, Hu Y, Yan Z, Zheng H, Yang G, Liu D, Tepel M, Gao P, Zhu Z (2020) Reducing NADPH synthesis counteracts diabetic nephropathy through restoration of AMPK activity in type 1 diabetic rats. Cell Rep 32:108207. - »Bioblast link«
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Skeletal muscle mitochondrial bioenergetics in men and women with type 1 diabetes
- from the O2k-Network »CA Toronto Perry CG«
- Monaco CMF, Bellissimo CA, Hughes MC, Ramos SV, Laham R, Perry CGR, Hawke TJ (2020) Sexual dimorphism in human skeletal muscle mitochondrial bioenergetics in response to type 1 diabetes. Am J Physiol Endocrinol Metab 318:E44-E51. - »Bioblast link«
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Bioblast alert 2021(06) - Fatty acid oxidation (FAO): 2021-03-19
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Approved FAO inhibitor Trimetazidine restricts tumor growth in oxidative lung carcinomas
- from the O2k-Network »FR Bordeaux Rossignol R«
- Amoedo ND, Sarlak S, Obre E, Esteves P, Bégueret H, Kieffer Y, Rousseau B, Dupis A, Izotte J, Bellance N, Dard L, Redonnet-Vernhet I, Punzi G, Rodrigues MF, Dumon E, Mafhouf W, Guyonnet-Dupérat V, Gales L, Palama T, Bellvert F, Dugot-Senan N, Claverol S, Baste JM, Lacombe D, Rezvani HR, Pierri CL, Mechta-Grigoriou F, Thumerel M, Rossignol R (2021) Targeting the mitochondrial trifunctional protein restrains tumor growth in oxidative lung carcinomas. J Clin Invest 131:e133081. - »Bioblast link«
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Quantifying FAO capacity (F-pathway) - avoid overestimation
Malate anaplerotic pathways are active in various tissues and cultured cells, which can lead to an overestimation of the F-pathway capacity. .
- Evaluate the malate anaplerotic pathway: »SUIT-027«
- Analyze F-pathway avoiding overestimation: »SUIT-002« and »SUIT-025«
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Bioblast alert 2021(05) - Bioenerg Commun & MitoFit Preprints: 2021-03-04
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Mitochondrial physiology in Parkinson’s disease (PD)
A COST Action MitoEAGLE contribution:
- Krako Jakovljevic N, Ebanks B, Chakrabarti L, Markovic I, Moisoi N (2021) Mitochondrial homeostasis in cellular models of Parkinson’s Disease. MitoFit Preprints 2021.4 doi:10.26124/mitofit:2021-0004
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Internal normalization of rate – what is the difference between flux control ratio and flux control efficiency?
A flux control ratio is the ratio of oxygen flux in a specific respiratory control state, normalized for maximum flux in a common reference state. Flux control efficiency expresses the control of respiration as a fractional change of oxygen flux or flow between protocol steps. Both values vary between theoretical lower and upper limits of 0 and 1.
- »Gnaiger 2020 BEC MitoPathways - Chapter 3«
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Submit manuscripts simultaneously to MitoFit Preprints and Bioenergetics Communications via the BEC website
A seamless process for taking MitoFit preprints to living BEC communications with open and published peer review.
- »Bioenergetics Communications«
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Bioblast alert 2021(04) - MitoFit Preprints: 2021-02-19
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Exploring the Q-junction in real-time
Simultaneous measurement of coenzyme Q redox state and respiration in isolated mitochondria:
- Komlodi T, Cardoso LHD, Doerrier C, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. MitoFit Preprints 2021.2. doi:10.26124/mitofit:2021-0002
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Diagnosis of mitochondrial disease in frozen samples
Measurement of respiratory function in cryopreserved muscle biopsies:
- Zuccolotto-dos-Reis FH, Andriao-Escarso SH, Araujo JS, Espreafico EM, Alberici LC, Sobreira CFR (2021) Acetyl CoA driven respiration in frozen muscle contributes to the diagnosis of mitochondrial disease. MitoFit Preprints 2021.3. doi:10.26124/mitofit:2021-0003
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Bioblast alert 2021(03) - ATP production P»: 2021-01-29
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SUIT protocols with Magnesium Green™ (MgG) can be used to study respiration and ATP production P»
MgG dye does not affect respiration of cardiac isolated mitochondria.
- Cardoso LHD, Doerrier C, Gnaiger E (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. MitoFit Preprints 2021.1. doi:10.26124/mitofit:2021-0001
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Measuring ATP-ADP exchange using the fluorophore Magnesium Green
- from the O2k-Network »HU Budapest Chinopoulos C«
- Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. »Bioblast link«
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Magnesium Green and P» – which sample preparations work?
Magnesium Green (MgG) can be used with mitochondrial preparations: isolated mitochondria, tissue homogenates, and permeabilized cells and tissues.
- »MgG« and »Publications MgG«
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Bioblast alert 2021(02) - Permeabilized fibers: 2021-01-21
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A quote from MitoPathways Chapter 3. Normalization:
Respiratory OXPHOS capacity P in human skeletal muscle ranges from 60 to 160 pmol∙s-1∙mg-1 based on wet muscle mass of permeabilized fibers. By comparison, mass-specific electron transfer capacity E is approximately 25 pmol∙s-1∙mg-1 in platelets and senescent fibroblasts and 40 to 80 pmol∙s-1∙mg-1 in PBMC, HEK, CEM, HUVEC, and young fibroblasts. ― Are these values comparable? - »Gnaiger 2020 BEC MitoPathways«.
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Statistical evaluation on replica in high-resolution respirometry with permeabilized skeletal muscle fibers
- from the O2k-Network »AU Melbourne Bishop DJ«
- Jacques M, Kuang J, Bishop DJ, Yan X, Alvarez-Romero J, Munson F, Garnham A, Papadimitriou I, Voisin S, Eynon N (2019) Mitochondrial respiration variability and simulations in human skeletal muscle: The Gene SMART study. FASEB J 34:2978-86. - »Bioblast link«
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Effect of temperature on mitochondrial respiratory capacity of cardiac permeabilized fibers
- from the O2k-Network »AT Innsbruck Gnaiger E« , »CA Rimouski Blier PU« and »CA Edmonton Lemieux H«
- Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. »Bioblast link«
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What is the optimal ADP concentration to evaluate OXPHOS capacity in permeabilized muscle fibers (pfi)?
For the evaluation of OXPHOS capacity, the limitation of respiratory fluxes must be avoided by using a kinetically-saturating ADP concentration. In permeabilized muscle fibers, an ADP concentration of 5 mM or higher is needed to reach kinetically-saturating levels.
- »Optimum ADP concentration in pfi«
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Bioblast alert 2021(01) - MitoPathways: the new BEC edition of the Blue Book: 2021-01-05
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The 5th edition of MitoPathways features new chapters on additivity of mitochondrial pathway capacities and protonmotive pressure – one step beyond ∆Ψ and the protonmotive force.
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Summary
Bioblast alert - previous issues
Stay alert
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Bioblast alert 2021
