Oroboros Ecosystem Agenda 2023 Archive

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Oroboros Ecosystem Agenda 2023 Archive

Mitochondria and Cell Research - the Oroboros Ecosystem
» Current Oroboros Ecosystem Agenda

Dec 2023

2023 Week 49
  • Dec- 04 Mo

  • Dec- 05 Tu
O2k-Open SupportInfo: improve Is DatLab 8 compatible with your operative system?

The DatLab 8 software is designed for 64-bit versions of Windows operating systems and does not run on MAC devices.
- »DatLab 8«
- communicated by »Leo Elettra« and »Baglivo Eleonora«

  • Dec- 06 We
MitoFit pdf "The capacity of FA oxidative phosphorylation (F-OXPHOS) with palmitoylcarnitine was up to 4 times higher than respiration with octanoylcarnitine. The optimal concentration of palmitoylcarnitine was 10 μM which corresponds to the total concentration of LC acylcarnitines in the brain."
  • Cecatto C, Cardoso LHD, Ozola M, Korzh S, Zvejniece L, Gukalova B, Doerrier C, Dambrova M, Gnaiger E, Makrecka-Kuka M, Liepinsh E (2023) Fatty acid β-oxidation in brain mitochondria: Insights from high-resolution respirometry in mouse, rat and Drosophila brain, ischemia and aging models. MitoFit Preprints 2023.10. https://doi.org/10.26124/mitofit:2023-0010
from the O2k-Network »AT Innsbruck Oroboros« and »LV Riga Liepins E«

  • Dec- 07 Th
O2k-Publications in the MiPMap "(…) L-Erg [L-Ergothioneine] treatment prevented and delayed cystine lithiasis in a cystinuria mouse model by increasing urinary cystine solubility and restored kidney GSH content and OXPHOS function."
  • Mayayo-Vallverdú C, López de Heredia M, Prat E, González L, Espino Guarch M, Vilches C, Muñoz L, Asensi MA, Serra C, Llebaria A, Casado M, Artuch R, Garrabou G, Garcia-Roves PM, Pallardó FV, Nunes V (2023) The antioxidant l-Ergothioneine prevents cystine lithiasis in the Slc7a9-/- mouse model of cystinuria »Bioblast link«
from the O2k-Network »ES Barcelona Moren C« and »ES Barcelona Garcia-Roves PM«

  • Dec- 08 Fr

2023 Week 50
  • Dec- 11 Mo - International Mountain Day
O2k-Open SupportInfo: improve Mountain slopes can be difficult, but O2 slopes can be smooth with DatLab

The default setting of Slope smoothing is 40 data points, corresponding to an interval of 80 s at a data recording interval of 2 s. DatLab 7 expresses it in data points and DatLab 8 in seconds.
- »Smoothing in Flux/Slope«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

  • Dec- 12 Tu
MitoFit pdf From preprint to Gold Open Access publication in J Biol Chem
  • Gnaiger E (2023) Complex II ambiguities ― FADH2 in the electron transfer system. »Bioblast link«

  • Dec- 13 We

  • Dec- 14 Th
O2k-Publications in the MiPMap "HCR [High capacity runner rats] showed a consistent and robust trend of higher maximal oxygen consumption compared to LCR [low capacity runner rats], especially for long chain FAs [fatty acids]."
  • Fleischman JY, Van den Bergh F, Collins NL, Bowers M, Beard DA, Burant CF (2023) Higher mitochondrial oxidative capacity is the primary molecular differentiator in muscle of rats with high and low intrinsic cardiorespiratory fitness. »Bioblast link«
from the O2k-Network »US MI Ann Arbor Beard DA«

  • Dec- 15 Fr

2023 Week 51
  • Dec- 18 Mo
»SBC 2023 Goa IN« Dec 18-20
Why add azide after ascorbate and TMPD when measuring Complex IV activity?

The O2 flux measured after the titration of ascorbate and TMPD is due to both CIV activity and autooxidation of these compounds. By subsequently inhibiting CIV activity with azide, the O2 flux due to autooxidation (chemical background) can be determined and subtracted from the initial measurement.
- »Complex IV«, »Chemical background « 
- communicated by »Grings Mateus« and »Timon-Gomez Alba« 

  • Dec- 19 Tu

  • Dec- 20 We
O2k-Publications in the MiPMap "SIRT4 [Sirtuin 4] knockdown increases mitochondrial capacity to oxidize fatty acids, decreasing LEAK respiration and further increasing mitochondrial elongation via its regulation of mitochondrial fusion."
  • Noone J, Rochfort KD, O'Sullivan F, O'Gorman DJ (2023) SIRT4 is a regulator of human skeletal muscle fatty acid metabolism influencing inner and outer mitochondrial membrane-mediated fusion. »Bioblast link«
from the O2k-Network »US FL Orlando Goodpaster BH« and »IE Dublin O Gorman D«

  • Dec- 21 Th

  • Dec- 22 Fr

2023 Week 52
  • Dec- 25 Mo Our office and technical support will be closed for the Christmas period until January 7, 2024.

  • Dec- 26 Tu

  • Dec- 27 We
O2k-Open SupportInfo: improve Do you observe a high signal at zero oxygen?

If the quality of your dithionite solution has already been checked, perform a leak current test to evaluate which electrical component is generating the out-of-scale signal.
- »Leak current test«
- communicated by »Baglivo Eleonora« and »Grings Mateus«

  • Dec- 28 Th

  • Dec- 29 Fr
O2k-Publications in the MiPMap "Leptin receptor signaling in Schwann cells adjusts catabolic processes, including mitochondrial respiration and autophagy, facilitating nerve repair."
  • Sundaram VK, Schütza V, Schröter NH, Backhaus A, Bilsing A, Joneck L, Seelbach A, Mutschler C, Gomez-Sanchez JA, Schäffner E, Sánchez EE, Akkermann D, Paul C, Schwagarus N, Müller S, Odle A, Childs G, Ewers D, Kungl T, Sitte M, Salinas G, Sereda MW, Nave KA, Schwab MH, Ost M, Arthur-Farraj P, Stassart RM, Fledrich R (2023) Adipo-glial signaling mediates metabolic adaptation in peripheral nerve regeneration. »Bioblast link«
from the O2k-Network »DE_Leipzip_Ost_M«

Nov 2023

2023 Week 44
  • Nov- 01 We

  • Nov- 02 Th

  • Nov- 03 Fr
O2k-Publications in the MiPMap "Peripheral impairments at the skeletal muscle level, namely reduced mitochondrial function and the presence of markers of impaired mitochondrial biogenesis emerged as major determinants of exercise intolerance and fatigue [in patients with post-acute sequelae of SARS-CoV-2 infection]"
  • Colosio M, Brocca L, Gatti M, Neri M, Crea E, Cadile F, Canepari M, Pellegrino MA, Polla B, Porcelli S, Bottinelli R (2023) Structural and functional impairments of skeletal muscle in patients with post-acute sequelae of SARS-CoV-2 infection. »Bioblast link«

2023 Week 45
  • Nov- 06 Mo

  • Nov- 07 Tu
O2k-Open SupportInfo: take care How often should I re-assemble the O2k-chamber?

We recommend disassembling, cleaning and re-assembling the O2k-chamber at least every 1-2 years. It is also necessary to disassemble it when there is persistent biological or protein contamination, medium leakage from the chamber, an oxygen leak of the chamber, or an unstable or unexpectedly high or low oxygen signal.
- »O2k-chamber assembly - possible problems« and »O2k-Videosupport: Insert O2k-chamber«
- communicated by »Timon-Gomez Alba« and »Schmitt Sabine«

  • Nov- 08 We

  • Nov- 09 Th

  • Nov- 10 Fr
When should I analyze my data as flux control ratios (FCRs)?

FCRs enable the comparison of samples normalized with various mitochondrial content parameters or when sample concentration is unknown. This is achieved by internally normalizing O2 fluxes in different respiratory states to the maximum flux in a common reference state.
- »Flux control ratio« 
- communicated by »Baglivo Eleonora« and »Timon-Gomez Alba« 

2023 Week 46
  • Nov- 13 Mo

  • Nov- 14 Tu - World Diabetes Day
O2k-Publications in the MiPMap "Liver [mitochondria] (...) adapt to metabolic challenges by increasing FAO-derived mitochondrial respiration in OBES [obesity model] and NASH [non-alcoholic steatohepatitis model], but by increasing both FAO- and TCA-derived respiration in DIAB [diabetes model]."
  • Dewidar B, Mastrototaro L, Englisch C, Ress C, Granata C, Rohbeck E, Pesta D, Heilmann G, Wolkersdorfer M, Esposito I, Reina Do Fundo M, Zivehe F, Yavas A, Roden M (2023) Alterations of hepatic energy metabolism in murine models of obesity, diabetes and fatty liver diseases. »Bioblast link«
from the O2k-Network »DE Duesseldorf Roden M« and »DE Cologne Pesta D«

  • Nov- 15 We

  • Nov- 16 Th - World Pancreatic Cancer Day
O2k-Publications in the MiPMap "To the advantage of PDAC [pancreatic ductal adenocarcinoma] patients, innovative and more effective therapeutics will be possible with a greater understanding of how pancreatic cancer cells control mitochondrial metabolism."
  • Padinharayil H, Rai V, George A (2023) Mitochondrial metabolism in pancreatic ductal adenocarcinoma: from mechanism-based perspectives to therapy. »Bioblast link«

  • Nov- 17 Fr

2023 Week 47
  • Nov- 20 Mo
O2k-Open SupportInfo: improve Do I need to change the filter set of the O2k-Fluo Smart-Module?

Because two different filter sets can be used with the blue Fluo-Sensors, filters should be changed according to the fluorophore that will be used. Extra filters are provided for replacement in case of damage.
- »O2k-Fluo Smart-Module manual« and »O2k-Fluo Smart-Module - Fluorescence dyes and settings« 
- communicated by »Cardoso Luiza« and »Timon-Gomez Alba«

  • Nov- 21 Tu

  • Nov- 22 We
Oliveira 2023 BEC
“The cautionary note provided by these studies emphasizes the need for careful consideration of which probes and conditions can be used when assessing mitochondrial physiology in Drosophila.”
from the O2k-Network »BR Rio de Janeiro Oliveira MF«

  • Nov- 23 Th
O2k-Publications in the MiPMap "Thoughtful design and execution of O2k experiments result in data that become a powerhouse for addressing many mitochondrial respiration research questions. "
  • Walsh MA, Musci RV, Jacobs RA, Hamilton KL (2023) A practical perspective on how to develop, implement, execute, and reproduce high-resolution respirometry experiments: The physiologist's guide to an Oroboros O2k »Bioblast link«
from the O2k-Network »US CO Colorado Springs Jacobs RA« and »US CO Fort Collins Hamilton K«

  • Nov- 24 Fr

2023 Week 48
  • Nov- 27 Mo

  • Nov- 28 Tu
Protocols that SUIT your research question

Using Substrate-Uncoupler-Inhibitor-Titration protocols you can study mitochondrial respiratory control through a sequence of coupling and pathway control states induced by multiple titrations within a single experimental assay.
- »SUIT protocols« and »SUITbrowser« 
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba« 

  • Nov- 29 We

  • Nov- 30 Th

  • Dec- 01 Fr World AIDS day
O2k-Publications in the MiPMap "We therefore hypothesized that the susceptibility to HIV-1 infection of FOXO1-inhibited resting T cells could be due to an increased cell metabolism. (...) Results obtained by high-resolution respirometry experiments of [human peripheral blood T cells] PBT (...) showed that [ROUTINE] respiration at the steady state was increased by [FOXO1 inhibitor] AS1842856."
  • Roux A, Leroy H, De Muylder B, Bracq L, Oussous S, Dusanter-Fourt I, Chougui G, Tacine R, Randriamampita C, Desjardins D, Le Grand R, Bouillaud F, Benichou S, Margottin-Goguet F, Cheynier R, Bismuth G, Mangeney M (2019) FOXO1 transcription factor plays a key role in T cell-HIV-1 interaction. »Bioblast link«
from the O2k-Network »FR Paris Bouillaud F«
»AMI 2023 Jhansi IN« Dec 1-3

Oct 2023

2023 Week 40
  • Oct- 02 Mo
O2k-Workshops.png Oroboros O2k-Workshop IOC162 Schroecken AT Oct 2-7

  • Oct- 03 Tu
O2k-Workshops.png Oroboros O2k-Workshop IOC162 Schroecken AT Oct 2-7

  • Oct- 04 We
O2k-Workshops.png Oroboros O2k-Workshop IOC162 Schroecken AT Oct 2-7

  • Oct- 05 Th - Breast Cancer Awareness Month
O2k-Publications in the MiPMap "GK-1 [immunomodulatory peptide] inhibits cellular respiration and induces mitochondrial uncoupling in breast tumors."
  • Cruz-Gregorio A, Aranda-Rivera AK, Aparicio-Trejo OE, Medina-Campos ON, Sciutto E, Fragoso G, Pedraza-Chaverri J (2022) GK-1 Induces oxidative stress, mitochondrial dysfunction, decreased membrane potential, and impaired autophagy flux in a mouse model of breast cancer. »Bioblast link«
from the O2k-Network »MX Mexico City Pedraza Chaverri J«
O2k-Workshops.png Oroboros O2k-Workshop IOC162 Schroecken AT Oct 2-7

  • Oct- 06 Fr
O2k-Workshops.png Oroboros O2k-Workshop IOC162 Schroecken AT Oct 2-7

  • Oct- 07 Sa
O2k-Workshops.png Oroboros O2k-Workshop IOC162 Schroecken AT Oct 2-7

2023 Week 41
  • Oct- 09 Mo

  • Oct- 10 Tu
What is the best fluorophore and concentration thereof for measuring mt-membrane potential?

It depends on sample characteristics - test the concentration of different fluorophores (safranin, TMRM and rhodamine 123) with your specific model to evaluate for inhibitory or uncoupling effects on respiration.
- »mt-membrane potential measurement« 
- communicated by »Grings Mateus« and »Cardoso Luiza« 

  • Oct- 11 We
The abbreviation 'Dith' has been used previously for Dithionite and is stepwise replaced by the shorter 'Dit'.

- »Dithionite, Dit«

  • Oct- 12 Th - World Arthritis Day
O2k-Publications in the MiPMap “Mitochondrial dysfunction did not seem to be a major contributing factor for insulin resistance in RA [rheumatoid arthritis]”
  • Moellering DR, Smith-Johnston K, Kelley C, Sammy MJ, Benedict J, Brock G, Johnson J, Baskin KK, Jarjour WN, Belury MA, Reiser PJ, Nagareddy PR, Hanaoka BY (2023) Association between skeletal muscle mitochondrial dysfunction and insulin resistance in patients with rheumatoid arthritis: a case-control study. »Bioblast link«
from the O2k-Network »US AL Birmingham Moellering_DR«

  • Oct- 13 Fr

2023 Week 42
  • Oct- 16 Mo
»2nd Workshop on Mitochondrial Functional Diagnostics Innsbruck AT«

  • Oct- 17 Tu
O2k-Open SupportInfo: improve Noticing higher values of J°1 when using the O2k-Chamber sV (0.5 mL) compared to the 2 mL O2k-Chamber?

The instrumental oxygen background flux, J°O2, is volume-dependent in the closed chamber; therefore, the values of J°1 are about four times higher in the sV chamber.
- »O2k-sV-Module«, »POS calibration-SOP«
- communicated by »Timon-Gomez Alba« and »Leo Elettra«

  • Oct- 18 We
»EU-METAHEART Kick-off meeting Brussels BE«

  • Oct- 19 Th
»Life Sciences Partnering 2023 Innsbruck AT«
O2k-Publications in the MiPMap "MDH2 is a metabolic switch rewiring [electron transfer system] and TCA fuelling"
  • Molinié T, Cougouilles E, David C, Cahoreau E, Portais JC, Mourier A (2022) MDH2 produced OAA is a metabolic switch rewiring the fuelling of respiratory chain and TCA cycle. »Bioblast link«
from the O2k-Network »FR Bordeaux Mourier A«

  • Oct- 20 Fr

2023 Week 43
  • Oct- 23 Mo
O2k-Feedback “Thanks for this excellent guidance and support thus far.”

Maarman Gerald J, from the O2k-Network Lab »ZA Cape Town Maarman GJ«
- »O2k-Feedback«

  • Oct- 24 Tu
MitoFit pdf A theoretical communication from Garcia Menendez on electromagnetic coupling hypothesis.
  • Garcia Menendez R (2023) Update and a new statement of the electromagnetic coupling hypothesis to explain the translocation of protons in mitochondria, bacteria and chloroplasts. MitoFit Preprints 2023.8. https://doi.org/10.26124/mitofit:2023-0008

  • Oct- 25 We

  • Oct- 26 Th
O2k-Publications in the MiPMap "In collegiate swimmers, (…) a maximal exercise bout (i.e., the bucket test) was associated with minimal changes in cellular-level IO2 [oxygen flow in isolated PBMCs]. In contrast, due to the robust mobilization of immune cells in response to the maximal exercise bout, the tissue-level IO2-Tissue [blood] was significantly elevated."
  • Stampley JE, Cho E, Wang H, Theall B, Johannsen NM, Spielmann G, Irving BA (2023) Impact of maximal exercise on immune cell mobilization and bioenergetics. »Bioblast link«
from the O2k-Network »US LA Baton Rouge Irving BA«

  • Oct- 27 Fr

2023 Week 44
  • Oct- 30 Mo

  • Oct- 31 Tu
How to interpret cytochrome c efficiency

Stimulation of OXPHOS or ET capacity upon addition of cytochrome c provides evidence of cytochrome c loss, indicating damage of the mitochondrial outer membrane, which could be caused by sample preparation or pathological conditions.
- »Cytochrome c control efficiency« 
- communicated by »Schmitt Sabine« and »Leo Elettra« 

Sep 2023

2023 Week 36
  • Sep- 04 Mo

  • Sep- 05 Tu
Higher concentrations of oligomycin can decrease the ET capacity due to an inhibitory effect

Use a lower oligomycin concentration (5-10 nM) to measure LEAK respiration without impairing ET capacity and test to find the optimum concentration for your specific model.
- »Oligomycin in high-resolution respirometry«, »Doerrier 2018 Methods Mol Biol« 
- communicated by »Timon-Gomez Alba« and »Grings Mateus« 

  • Sep- 06 We

  • Sep- 07 Th
O2k-Publications in the MiPMap "...enhancing mitochondrial function by either overexpressing parkin or bypassing defective mitochondrial complexes has significant beneficial effects in a fly model of HD [Huntington's disease], rescuing neurodegeneration and enhancing viability and lifespan."
  • Campesan S, Del Popolo I, Marcou K, Straatman-Iwanowska A, Repici M, Boytcheva KV, Cotton VE, Allcock N, Rosato E, Kyriacou CP, Giorgini F (2023) Bypassing mitochondrial defects rescues Huntington's phenotypes in Drosophila. »Bioblast link«
from the O2k-Network »UK Leicester Giorgini F«

  • Sep- 08 Fr

2023 Week 37
  • Sep- 11 Mo
O2k-Open SupportInfo: improve Barometric pressure displaying as 0 kPa in the O2 calibration window?

In many cases, this can be solved by by switching off and unplugging the O2k for several minutes.
- »O2k-Barometric Pressure Transducer«
- communicated by »Schmitt Sabine« and »Grings Mateus«

  • Sep- 12 Tu

  • Sep- 13 We

  • Sep- 14 Th
O2k-Publications in the MiPMap "The higher ROS release rates observed in both the muscle and liver of older individuals may suggest that increased ROS release is evidence of mitochondrial dysfunction during ageing in finches."
  • Salmón P, Millet C, Selman C, Monaghan P, Dawson NJ (2023) Tissue-specific reductions in mitochondrial efficiency and increased ROS release rates during ageing in zebra finches, Taeniopygia guttata. »Bioblast link«
from the O2k-Network »DE Wilhelmshaven Salmon P«, »UK Glasgow Metcalfe NB«

  • Sep- 15 Fr - World Lymphoma Awareness Day
O2k-Publications in the MiPMap "Respirometry assays confirmed that OXPHOShigh Richter transformation cells had a 3.5-fold higher oxygen consumption at ROUTINE respiration and fivefold higher electron transfer system capacity compared to chronic lymphocytic leukemia"
  • Nadeu F, Royo R, Massoni-Badosa R, Playa-Albinyana H, Garcia-Torre B, et al (2022) Detection of early seeding of Richter transformation in chronic lymphocytic leukemia. »Bioblast link«
from the O2k-Network »ES Barcelona Garcia-Roves PM« and »ES Barcelona IDIBAPS Hospital Clinic«

2023 Week 38
  • Sep- 18 Mo
World Mitochondrial Disease Week logo.jpg World Mitochondrial Disease Week – Sep 18-24

World Mitochondrial Disease Week raises awareness of mitochondrial disease through educational, fundraising and advocacy activities.
»See what is happening near you«

Our Chinese distributor Huawei came to visit us in Innsbruck today.
»CN Beijing Huawei«
Huawei and Oroboros

  • Sep- 19 Tu
O2k-Publications in the MiPMap In 1962, the first report of a patient with a mitochondrial disease was published.
  • Luft R, Ikkos D, Palmieri G, Ernster L, Afzelius B (1962). A case of severe hypermetabolism of nonthyroid origin with a defect in the maintenance of mitochondrial respiratory control: a correlated clinical, biochemical, and morphological study. »Bioblast link«

  • Sep- 20 We

  • Sep- 21 Th
O2k-Network.png The first mitochondrial DNA mutations associated with disease were described in 1988.

These discoveries were a game changer in the field of mitochondrial diseases. 

  • Holt IJ, Harding A E, Morgan-Hughes JA (1988) Deletions of muscle mitochondrial DNA in patients with mitochondrial myopathies. »Bioblast link«
  • Wallace DC, Singh G, Lott MT, Hodge JA, Schurr TG, Lezza AM, Elsas LJ 2nd, Nikoskelainen EK (1988) Mitochondrial DNA mutation associated with Leber's hereditary optic neuropathy. »Bioblast link«
  • Zeviani M, Moraes CT, DiMauro S, Nakase H, Bonilla E, Schon EA, Rowland LP (1988) Deletions of mitochondrial DNA in Kearns-Sayre syndrome. »Bioblast link«

  • Sep- 22 Fr

2023 Week 39
  • Sep- 25 Mo

  • Sep- 26 Tu
Do you know the difference between uncoupled, noncoupled and dyscoupled respiration?

Noncoupled respiration is induced experimentally for evaluation of ET capacity, while uncoupling and dyscoupling are respectively caused by physiological and pathological conditions that exert an influence on proton leak and slip.
- »Noncoupled respiration«, »BEC 2020.01« 
- communicated by »Leo Elettra« and »Cardoso Luiza« 

  • Sep- 27 We

  • Sep- 28 Th
O2k-Publications in the MiPMap "... systemic transplantation of young MDSPCs [muscle-derived stem/progenitor cells] into progeroid ZMPSTE24-deficient mice preserves skeletal muscle mitochondrial function and fatigue-resistant fibers in a sex-specific manner"
  • Thompson SD, Barrett KL, Rugel CL, Redmond R, Rudofski A, Kurian J, Curtin JL, Dayanidhi S, Lavasani M (2023) Sex-specific preservation of neuromuscular function and metabolism following systemic transplantation of multipotent adult stem cells in a murine model of progeria. »Bioblast link«
from the O2k-Network »US IL Chicago Dayanidhi S«

  • Sep- 29 Fr

Aug 2023

2023 Week 31
  • Jul- 31 Mo
O2k-Feedback “I really appreciate the amazing customer support of your company.”

Jean-Philippe Leduc-Gaudet, PhD from Université du Québec à Trois-Rivières, CA
- »O2k-Feedback«

  • Aug- 01 Tu
Respiration might be inhibited in the presence of high concentrations of some saccharides due to the Crabtree effect

It is observed in multiple cell types, such as tumor cells, bacteria or yeast, and it can be measured using SUIT-003 protocols.
- »Crabtree effect«, »SUIT-003« 
- communicated by »Timon-Gomez Alba« and »Leo Elettra« 

  • Aug- 02 We

  • Aug- 03 Th
O2k-Publications in the MiPMap "Hepatocyte-derived cells maintain cellular and mitochondrial Ca2+ concentrations within strictly controlled ranges, maximizing electron transport capacity by promoting the Goldilocks Ca2+ “sweet spot” for oxidative phosphorylation activity: neither too much nor too little, but just right."
  • Vilas-Boas EA, Cabral-Costa JV, Ramos VM, Caldeira da Silva CC, Kowaltowski AJ (2023) Goldilocks calcium concentrations and the regulation of oxidative phosphorylation: too much, too little, or just right. »Bioblast link«
from the O2k-Network »BR Sao Paulo Kowaltowski AJ«

  • Aug- 04 Fr

2023 Week 32
  • Aug- 07 Mo

  • Aug- 08 Tu
Rodriguez 2023 BEC
An overview of the “do’s and don’ts” when it comes to assessing mitochondrial function in Drosophila
  • Rodríguez E, Bettinazzi S, Inwongwan S, Camus MF, Lane N (2023) Harmonizing protocols to measure Drosophila respiratory function in mitochondrial preparations. Bioenerg Commun 2023.3. https://doi.org/10.26124/bec:2023-0003
from the O2k-Network »UK London Lane N«

  • Aug- 09 We

  • Aug- 10 Th
O2k-Publications in the MiPMap "Reducing lipid hydroxides by GPX4 overexpression ameliorates the majority of muscle atrophy and weakness phenotypes present in mouse models of sarcopenia"
  • Czyżowska A, Brown J, Xu H, Sataranatarajan K, Kinter M, Tyrell VJ, O'Donnell VB, Van Remmen H (2023) Elevated phospholipid hydroperoxide glutathione peroxidase (GPX4) expression modulates oxylipin formation and inhibits age-related skeletal muscle atrophy and weakness. »Bioblast link«
from the O2k-Network »US OK Oklahoma City Van Remmen H«

  • Aug- 11 Fr

2023 Week 33
  • Aug- 14 Mo
O2k-Open SupportInfo: improve What do you do if values, symbols, and/or units are not correctly displayed in the DatLab software?

It is possible to retrieve the values, symbols and/or correct units by updating the Windows control panel settings.
- » Troubleshooting for problems with text in DatLab«
- communicated by »Cecatto Cristiane« and »Timon-Gomez Alba«

  • Aug- 15 Tu

  • Aug- 16 We

  • Aug- 17 Th
O2k-Publications in the MiPMap "... species-specific differences in cardiac metabolic capacity [...] may influence the acclimation potential of notothenioid fishes to changing environmental conditions."
  • Mandic M, Frazier AJ, Naslund AW, Todgham AE (2022) A comparative and ontogenetic examination of mitochondrial function in antarctic notothenioid species. »Bioblast link«

  • Aug- 18 Fr

2023 Week 34
  • Aug- 21 Mo

  • Aug- 22 Tu
Are pyruvate, glutamate, and malate substrates of Complex I?

No, NADH is a Complex I substrate. However, pyruvate, glutamate and malate are used in high-resolution respirometry as substrates for mitochondrial dehydrogenases to generate NADH in the mitochondrial matrix because NADH cannot cross the mitochondrial inner membrane.
- »NADH electron transfer-pathway state« and »Complex I« 
- communicated by »Cardoso Luiza« and »Grings Mateus«

  • Aug- 23 We

  • Aug- 24 Th
FAT4BRAIN "Understanding acylcarnitine metabolic and signaling pathways provides a better rationale for selecting targets for therapeutic interventions."
  • Dambrova M, Makrecka-Kuka M, Kuka J, Vilskersts R, Nordberg D, Attwood MM, Smesny S, Sen ZD, Guo AC, Oler E, Tian S, Zheng J, Wishart DS, Liepinsh E, Schioth HB. (2022) Acylcarnitines: Nomenclature, Biomarkers, Therapeutic Potential, Drug Targets, and Clinical Trials. »Bioblast link«
from the O2k-Network »LV Riga Liepins E«

  • Aug- 25 Fr

2023 Week 35
  • Aug- 28 Mo
O2k-Open SupportInfo: improve Can I compare data sets recorded with different sensor gain settings?

Oxygen signals obtained after proper calibration are independent of the gain settings, therefore oxygen concentrations (and fluxes) can be compared, as long as the corresponding air and zero calibrations are applied.
- »Gain of the O2 channel«
- communicated by »Baglivo Eleonora« and »Leo Elettra«

  • Aug- 29 Tu
MitoFit pdf "Altogether, our results show that chronic hypoxia optimizes the efficiency of mitochondrial ATP synthesis in the retrosplenial cortex of mice. Contrastingly, rats sustain the production of ATP only by increasing mitochondrial content and altering mitochondrial dynamics, suggesting drastic mitochondrial malfunctions."
  • Arias-Reyes C, Aliaga-Raduán F, Pinto-Aparicio R, Joseph V, Soliz J (2023) Mitochondrial plasticity in the retrosplenial cortex enhances ATP synthesis during acclimatization to hypoxia in mice but not in rats. MitoFit Preprints 2023.6. https://doi.org/10.26124/mitofit:2023-0006
from the O2k-Network »CA Quebec Soliz J«

  • Aug- 30 We

  • Aug- 31 Th
O2k-Publications in the MiPMap "Our study showed that salinity modulates the mitochondrial responses to long-term and acute hypoxia exposure in an euryhaline intertidal bivalve C. gigas."
  • Steffen JBM, Sokolov EP, Sokolova IM (2023) Combined effects of salinity and intermittent hypoxia on mitochondrial capacity and reactive oxygen species efflux in the Pacific oyster, Crassostrea gigas »Bioblast link«
from the O2k-Network »DE Rostock Sokolova I«

  • Sep- 01 Fr

Jul 2023

2023 Week 27
  • Jul- 03 Mo

  • Jul- 04 Tu
The function of mitochondrial respiratory supercomplexes is still under discussion

Although their structure has been described in different species, their function is still unclear. Come to MiPschool 2023 to find out about the latest discoveries.
- »Respiratory complexes and supercomplexes« and »MiPschool 2023« 
- communicated by »Timon-Gomez Alba« and »Cecatto Cristiane«

  • Jul- 05 We

  • Jul- 06 Th
O2k-Publications in the MiPMap "G3 MB [Group 3 medulloblastoma] cells demonstrate exquisite sensitivity towards inhibitors of complex-I in the electron transport [system (ETS)] but not to other metabolism-targeting agents, including glycolytic inhibitors."
  • Martell E, Kuzmychova H, Kaul E, Senthil H, Chowdhury SR, Morrison LC, Fresnoza A, Zagozewski J, Venugopal C, Anderson CM, Singh SK, Banerji V, Werbowetski-Ogilvie TE, Sharif T (2023) Metabolism-based targeting of MYC via MPC-SOD2 axis-mediated oxidation promotes cellular differentiation in group 3 medulloblastoma. »Bioblast link«
from the O2k-Network »CA Winnipeg Banerji V«

  • Jul- 07 Fr

2023 Week 28
  • Jul- 10 Mo
»PBMC-Workshop Innsbruck AT«

  • Jul- 11 Tu
Here is a system of terms and abbreviations for mitochondrial respiratory pathway and coupling control states.

Although states and rates are distinguished by different symbols for coupling control states, such a distinction is not suggested for pathway control states and rates to avoid handing and overwhelming number of different symbols.
- »MitoPedia: Pathway and coupling control states«

  • Jul- 12 We
Is the ambiguity crisis a contemporary crisis comparable to the credibility or reproducibility crisis?

This is discussed in Gnaiger E (2023) Complex II ambiguities ― FADH2 in the electron transfer system. https://doi.org/10.26124/mitofit:2023-0003.v6
- »MitoPedia: Ambiguity crisis«

  • Jul- 13 Th
O2k-brief repository Triplefin fish species have different ROS production and electron leakage according to their tolerance to hypoxia.
  • Devaux JBL, Hedges CP, Birch N, Herbert N, Renshaw GMC, Hickey AJR (2023) Electron transfer and ROS production in brain mitochondria of intertidal and subtidal triplefin fish (Tripterygiidae). »Bioblast link«

  • Jul- 14 Fr

  • Jul- 15 Sa
»2nd Mitochondria Conference 2023 Lisbon PT« Jul 15-18

2023 Week 29
  • Jul- 17 Mo
O2k-Open SupportInfo: take care Are you planning to perform several measurements in one day using the same O2k-chamber?

To avoid any possible contamination by inhibitors in the subsequent measurements, the O2k-chamber needs to be cleaned each time with the standard protocol AfterUse or AfterUse_inhibitors.
- »O2k-cleaning - Sections 2.3 and 2.4«
- communicated by »Timon-Gomez Alba« and »Cecatto Cristiane«

  • Jul- 18 Tu

  • Jul- 19 We

  • Jul- 20 Th
O2k-Publications in the MiPMap "A cancer-selective Na+ increase correlated with HCC [hepatocellular carcinoma] cell killing, and a slower growth rate of HCC xenograft tumors."
  • Clemente N, Baroni S, Fiorilla S, Tasso F, Reano S, Borsotti C, Ruggiero MR, Alchera E, Corrazzari M, Walker G, Follenzi A, Crich SG, Carini R (2023) Boosting intracellular sodium selectively kills hepatocarcinoma cells and induces hepatocellular carcinoma tumor shrinkage in mice. »Bioblast link«
from the O2k-Network »IT Novara Filigheddu N«

  • Jul- 21 Fr

  • Jul- 22 Sa

  • Jul- 23 Su
MiP school logo 2023.png MiPschool Obergurgl 2023 Jul 23-27

2023 Week 30
  • Jul- 24 Mo
MiP school logo 2023.png MiPschool Obergurgl 2023 Jul 23-27

  • Jul- 25 Tu
MiP school logo 2023.png MiPschool Obergurgl 2023 Jul 23-27
Body mass excess (BME) or body mass index (BMI)?

BME provides an evidence-based index of underweight, overweight and obesity across all heights and ages, in contrast to BMI. Try our BME calculator.
- »Body mass excess calculator« and »Body mass excess« 
- communicated by »Timon-Gomez Alba« and »Cecatto Cristiane«

  • Jul- 26 We
MiP school logo 2023.png MiPschool Obergurgl 2023 Jul 23-27

  • Jul- 27 Th
MiP school logo 2023.png MiPschool Obergurgl 2023 Jul 23-27
MiP conference logo 2023.png 15th MiPconference: Bioenergetics Communications on mitObesity and Healthy Aging Obergurgl 2023 Jul 27-30
O2k-brief repository Mitochondrial function abnormalities in patients with heart failure with preserved ejection fraction are associated with exercise intolerance and represent novel therapeutic targets.
  • Scandalis L, Kitzman DW, Nicklas BJ, Lyles M, Brubaker P, Nelson MB, Gordon M, Stone J, Bergstrom J, Neufer PD, Gnaiger E, Molina AJA (2023) Skeletal muscle mitochondrial respiration and exercise intolerance in patients with heart failure with preserved ejection fraction. »Bioblast link«

  • Jul- 28 Fr
MiP conference logo 2023.png 15th MiPconference: Bioenergetics Communications on mitObesity and Healthy Aging Obergurgl 2023 Jul 27-30

  • Jul- 29 Sa
MiP conference logo 2023.png 15th MiPconference: Bioenergetics Communications on mitObesity and Healthy Aging Obergurgl 2023 Jul 27-30

  • Jul- 30 Su
MiP conference logo 2023.png 15th MiPconference: Bioenergetics Communications on mitObesity and Healthy Aging Obergurgl 2023 Jul 27-30

Jun 2023

2023 Week 23
  • Jun- 05 Mo

  • Jun- 06 Tu
»Health Innovation Summit 2023 Vienna AT«
13:30-14:30 Erich Gnaiger discussion panel member at Health Innovation Summit 2023 Vienna AT - The BMI gender data gap: Cellular energy metabolism is fired by mitochondria. Their respiratory capacity decreases with obesity. But the WHO defines obesity with an adult-white-male medicine bias inherent in the body mass index.

  • Jun- 07 We
»ESCI 2023 Prague CZ« Jun 7-9

  • Jun- 08 Th
»International GIBB Meeting 2023 Riva del Garda IT« Jun 8-10
13:00 Baglivo Eleonora poster session at ESCI 2023 Prague CZ - The cytochrome c test for respirometric quality control of the integrity of the mitochondrial outer membrane - Session S01 - Mitochondrial Biology
13:00 Leo Elettra poster session at ESCI 2023 Prague CZ - High-resolution respirometry is comparable in 0.5 mL and 2.0 mL chamber volumes: studies with platelets, permeabilized fibroblasts, and isolated mitochondria - Session S01 - Mitochondrial Biology
16:00-16:15 Timon-Gomez Alba oral presentation at International GIBB Meeting 2023 Riva del Garda IT - A reference substrate-uncoupler-inhibitor titration protocol to create a mitochondrial pathway and coupling control database - Session 1 - Redox reactions and dynamics of energy-transducing membranes

  • Jun- 09 Fr
14:30-15:00 Gnaiger Erich Plenary lecture 3 at International GIBB Meeting 2023 Riva del Garda IT - Nonlinearity of the proton leak and membrane potential - protonmotive pressure as a unifying concept

  • Jun- 10 Sa

  • Jun- 11 Su
»EUROMIT2023 Bologna IT« Jun 11-15
2023 Week 24
  • Jun- 12 Mo
»EUROMIT2023 Bologna IT« Jun 11-15
15:30 Timon-Gomez Alba poster session at EUROMIT2023 Bologna IT - Mitochondrial injury in warm ischemia studied by high-resolution respirometry - Session topic: Modelling pathogenic mechanisms: OXPHOS, metabolic rewiring and tissue specificity. Poster #11
18:00 Gnaiger Erich poster session at EUROMIT2023 Bologna IT - Off-target effects of etomoxir: inhibition of mitochondrial Complex I and fatty acid oxidation - Session topic: Modelling pathogenic mechanisms: OXPHOS, metabolic rewiring and tissue specificity. Poster #135

  • Jun- 13 Tu
12:45-13:45 Gnaiger Erich lunch workshop at EUROMIT2023 Bologna IT - Mitochondrial bioenergetics - a quantitative analytical and diagnostic approach - Sala Europa

  • Jun- 14 We

  • Jun- 15 Th
O2k-Publications in the MiPMap "Obesity has an opposing association with mitochondrial respiration in adipose- and liver tissue with no overall association with NAFLD (nonalcoholic fatty liver disease) severity."
  • Pedersen JS, Rygg MO, Chrøis K, Sustarsic EG, Gerhart-Hines Z, Wever Albrechtsen NJ, Serizawa RR, Kristiansen VB, Basse AL, Boilesen AEB, Olsen BH, Hansen T, Gluud LL, Madsbad S, Larsen S, Bendtsen F, Dela F (2022) Influence of NAFLD and bariatric surgery on hepatic and adipose tissue mitochondrial biogenesis and respiration. »Bioblast link«
from the O2k-Network »DK Copenhagen Gerhart-Hines Z« and »DK Copenhagen Larsen S«

  • Jun- 16 Fr
2023 Week 25
  • Jun- 19 Mo
O2k-Workshops.png Oroboros O2k-Workshop IOC Schroecken AT Jun 19-24

  • Jun- 20 Tu
O2k-Workshops.png Oroboros O2k-Workshop IOC Schroecken AT Jun 19-24
Decline of mitochondrial fitness in overweight states is a biomarker of the systemic mitObesity syndrome, providing a mechanistic link between common obesity and associated chronic comorbidities.

Body mass excess provides an evidence-based index of underweight, overweight and obesity across all heights and ages, applicable to all populations for which the healthy reference baseline is established.
- »Body mass excess and mitObesity«

  • Jun- 21 We
O2k-Workshops.png Oroboros O2k-Workshop IOC Schroecken AT Jun 19-24

  • Jun- 22 Th
O2k-Workshops.png Oroboros O2k-Workshop IOC Schroecken AT Jun 19-24
O2k-Publications in the MiPMap "BAM15 is a tolerable and efficacious protonophore that protects against diet‐induced obesity."
  • Axelrod CL, King WT, Davuluri G, Noland RC, Hall J, Hull M, Dantas WS, Zunica ER, Alexopoulos SJ, Hoehn KL, Langohr I, Stadler K, Doyle H, Schmidt E, Nieuwoudt S, Fitzgerald K, Pergola K, Fujioka H, Mey JT, Fealy C, Mulya A, Beyl R, Hoppel CL, Kirwan JP (2021) BAM15-mediated mitochondrial uncoupling protects against obesity and improves glycemic control. »Bioblast link«
from the O2k-Network »US LA Baton Rouge Noland RC« and »US OH Cleveland Hoppel CL«

  • Jun- 23 Fr
O2k-Workshops.png Oroboros O2k-Workshop IOC Schroecken AT Jun 19-24

  • Jun- 24 Sa
O2k-Workshops.png Oroboros O2k-Workshop IOC Schroecken AT Jun 19-24

2023 Week 26
  • Jun- 26 Mo
O2k-Feedback “Thank you very much for your thorough help. I appreciate the time you took to provide me with all the answers and supportive literature to realize the potential of the O2k-respirometer in bioenergetics research”

Badr Ibrahim, PhD from Betterhumans Inc., US
- »O2k-Feedback«

  • Jun- 27 Tu

  • Jun- 28 We

  • Jun- 29 Th
O2k-Publications in the MiPMap "This study reinforces the strict aerobic status of M. tuberculosis and indicates that the therapeutic value of inhibiting the terminal oxidases is relevant even under nano-oxic conditions found in TB [tuberculosis] lung granulomas."
  • Kalia NP, Singh S, Hards K, Cheung CY, Sviriaeva E, Banaei-Esfahani A, Aebersold R, Berney M, Cook GM, Pethe K (2023) M. tuberculosis relies on trace oxygen to maintain energy homeostasis and survive in hypoxic environments. »Bioblast link«

  • Jun- 30 Fr

May 2023

2023 Week 18
  • May- 01 Mo

  • May- 02 Tu
O2k-Publications in the MiPMap "Mitochondria–nucleus communication and its integration within the maintenance of cellular homeostasis constitute key determinants of cellular fate and organism health in the context of cellular stress, homeostasis perturbation, and disease"
  • Temelie M, Talpur R, Dominguez-Prieto M, Dantas Silva A, Cenusa C, Craciun L, Savu DI, Moisoi N (2023) Impaired integrated stress response and mitochondrial integrity modulate genotoxic stress impact and lower the threshold for immune signalling. »Bioblast link«
from the O2k-Network »UK Leicester Moisoi N«

  • May- 03 We

  • May- 04 Th

  • May- 05 Fr

2023 Week 19
  • May- 08 Mo

  • May- 09 Tu
O2k-Open SupportInfo: take care Does your polarographic oxygen sensor (POS) need a deep clean?

A POS with a very dark anode might need several rounds of ammonia cleaning, possibly including prolonged exposure overnight to restore performance specifications.
- »POS service«
- communicated by »Cecatto Cristiane« and »Leo Elettra«

  • May- 10 We

  • May- 11 Th

  • May- 12 Fr

2023 Week 20
  • May- 15 Mo

  • May- 16 Tu - The San Francisco Declaration on Research Assessment (DORA) turns 10 years

16:00 Erich Gnaiger presenting: Implementing DORA principles by publishing in Bioenergetics Communications - beyond counting papers

  • May- 17 We

  • May- 18 Th

  • May- 19 Fr

2023 Week 21
  • May- 22 Mo - Type 2 Diabetes Prevention Week

  • May- 23 Tu
»Pint of Science 2023 Innsbruck AT«
19:00 Erich Gnaiger presenting at Pint of Science 2023 Innsbruck AT - Body mass excess and decline of mitochondrial fitness

  • May- 24 We

  • May- 25 Th
O2k-Publications in the MiPMap "Such markers [of mitochondrial function] could help to identify metabolic changes before the disease becomes irreversible and at a stage where therapeutic and lifestyle intervention could be more effective, thus reducing the burden of the health and economic costs of this epidemic."
  • Calabria E, Muollo V, Cavedon V, Capovin T, Saccenti L, Passarotti F, Ghiotto L, Milanese C, Gelati M, Rudi D, Salvagno GL, Lippi G, Tam E, Schena F, Pogliaghi S (2023) Type 2 diabetes related mitochondrial defects in peripheral mononucleated blood cells from overweight postmenopausal women. »Bioblast link«
from the O2k-Network »IT Verona Venturelli M«

  • May- 26 Fr

2023 Week 22
  • May- 29 Mo

  • May- 30 Tu
Registration deadline for »EUROMIT2023 Bologna IT«

  • May- 31 We
Measuring fatty acid oxidation (FAO)-linked respiration requires a low concentration of malate

In the absence of malate, accumulation of Acetyl-CoA inhibits FAO.
- »Fatty acid oxidation pathway control state«, »Mitochondrial Pathways: Chapter 5« 
- communicated by »Cecatto Cristiane« and »Cardoso Luiza« 

  • Jun- 01 Th

  • Jun- 02 Fr

Apr 2023

2023 Week 14
  • Apr- 03 Mo
Abstract closing date extended to April 16th for »MiPschool Obergurgl 2023« & »MiP2023 Obergurgl AT«
»PDBEB 2023 Coimbra PT« Apr 03-14

  • Apr- 04 Tu

  • Apr- 05 We

  • Apr- 06 Th
MitoFit pdf Oliveira and Couto-Lima provide evidence for the importance of local stock centers in sustaining the substantial increase in the output of Drosophila mitochondrial research worldwide in recent decades.
from the O2k-Network »BR Jaboticabal Oliveira MT«

  • Apr- 07 Fr

2023 Week 15
  • Apr- 10 Mo

  • Apr- 11 Tu
MitoFit pdf "Our findings suggest that O2 control of mitochondrial oxidative phosphorylation can relay changes in respiration and reduce mitochondrial Ca2+ uptake... [which] may influence how muscle mitochondria adapt after contractions in conditions of hypoxia."
  • Donnelly C, Komlódi T, Cecatto C, Cardoso LHD, Compagnion AC, Matera A, Tavernari D, Zanou N, Kayser B, Gnaiger E, Place N (2023) Functional hypoxia reduces mitochondrial calcium uptake. MitoFit Preprints 2023.2. https://doi.org/10.26124/mitofit:2023-0002
from the O2k-Network »AT Innsbruck Oroboros«, »CH Lausanne Place N«, »HU Budapest Tretter L«

  • Apr- 12 We
09:30 (GMT) Erich Gnaiger presenting at PDBEB 2023 Coimbra PT - Off-target effects of etomoxir: inhibition of mitochondrial Complex I and fatty acid oxidation
10:15 (GMT) Sabine Schmitt presenting at PDBEB 2023 Coimbra PT - Contribution of fatty acid oxidation to respiratory control in brain mitochondria

  • Apr- 13 Th
»ATSPB 2023 Hall in Tirol AT« Apr 13-15

  • Apr- 14 Fr

  • Apr- 15 Sa

  • Apr-16 Su
Abstract submission deadline for »MiPschool Obergurgl 2023« & »MiP2023 Obergurgl AT«
Early bird registration deadline for »EUROMIT2023 Bologna IT«

2023 Week 16
  • Apr- 17 Mo
MitoFit pdf “The ambiguities surrounding Complex II in the literature call for quality control, to secure scientific standards in current communications on bioenergetics and support adequate clinical applications.”
from the O2k-Network »AT Innsbruck Oroboros«

  • Apr- 18 Tu

  • Apr- 19 We
O2k-Workshops.png FAT4BRAIN 159th O2k-Workshop

  • Apr- 20 Th
FAT4BRAIN »FAT4BRAIN Symposium Riga LV«  April 20-21

  • Apr- 21 Fr

2023 Week 17
  • Apr- 24 Mo
NextGenO2k logo.jpg  »Oroboros distributor training 2023 Innsbruck AT« April 24-25

  • Apr- 25 Tu
MitoFit pdf “The variety of approaches to studying Drosophila mitochondrial function in the circa 60 studies surveyed over a 12-year period calls for a more careful consideration of the methodological approach tailored to the research questions.”
  • Rodríguez E, Bettinazzi S, Inwongwan S, Camus MF, Lane N (2023) Harmonising protocols to measure Drosophila respiratory function in mitochondrial preparations. MitoFit Preprints 2023.4. https://doi.org/10.26124/mitofit:2023-0004
from the O2k-Network »UK London Lane N«

  • Apr- 26 We

  • Apr- 27 Th

  • Apr- 28 Fr

  • Apr- 29 Sa

  • Apr-30 Su
Early registration deadline for »MiPschool Obergurgl 2023« & »MiP2023 Obergurgl AT«

Mar 2023

  • Mar- 01 We

O2k-Workshops.png 158th Oroboros O2k-Coaching Days - Basic Feb 27 - Mar 1

  • Mar- 02 Th

  • Mar- 03 Fr - World Wildlife Day
O2k-Publications in the MiPMap "[...]adaptive changes in the physiology of specific mitochondrial fractions can help overcome the metabolic challenges of life in the harsh environment at high altitude."
  • Dawson NJ, Scott GR (2022) Adaptive increases in respiratory capacity and O2 affinity of subsarcolemmal mitochondria from skeletal muscle of high-altitude deer mice. »Bioblast link«
from the O2k-Network »CA Hamilton Scott GR«

2023 Week 10
  • Mar- 06 Mo
Bellissimo 2022 BEC
Natural cycling of ADP with ATP attenuates mitochondrial permeability transition in permeabilized muscle fibres: can repeating mtPT assessments with and without adenylates provide new insights?
  • Bellissimo CA, Soendergaard S, Hughes MC, Ramos SV, Larsen S, Perry CGR (2022) The influence of adenylate cycling on mitochondrial calcium-induced permeability transition in permeabilized skeletal muscle fibers. Bioenerg Commun 2023.1. https://doi.org/10.26124/bec:2023-0001
from the O2k-Network »CA Toronto Perry CG« and »DK Copenhagen Larsen S«

  • Mar- 07 Tu
Abstract deadline for »EUROMIT2023 Bologna IT«

  • Mar- 08 We

  • Mar- 09 Th

  • Mar- 10 Fr - World Kidney Day
O2k-Publications in the MiPMap "...subcapsular administration of MSC-derived EVs [extracellular vesicles secreted by adipose tissue mesenchymal cells] at the moment of reperfusion after ischemia totally recovers the normal ROS formation by mitochondria from renal proximal tubule cells in all functional respiratory states"
  • Lopes JA, Collino F, Rodrigues-Ferreira C, Sampaio LDS, Costa-Sarmento G, Wendt CHC, Almeida FP, Miranda KR, Kasai-Brunswick TH, Lindoso RS, Vieyra A (2022) Early effects of extracellular vesicles secreted by adipose tissue mesenchymal cells in renal ischemia followed by reperfusion: mechanisms rely on a decrease in mitochondrial anion superoxide production »Bioblast link«
from the O2k-Network »BR Rio de Janeiro Vieyra A«

2023 Week 11
  • Mar- 13 Mo

  • Mar- 14 Tu
Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?

- »Setting the oxygen concentration«

  • Mar- 15 We

  • Mar- 16 Th

  • Mar- 17 Fr

2023 Week 12
  • Mar- 20 Mo

  • Mar- 21 Tu
O2k-Open SupportWarning: loss of data Did you know that a humid environment and/or liquid spillage may lead to a high raw POS signal [V]?

See how to clean and dry the electrical connections of the OroboPOS-Connector.
- »Cleaning the electrical connections«
- communicated by »Cecatto Cristiane« and »Baglivo Eleonora«

  • Mar- 22 We

  • Mar- 23 Th

  • Mar- 24 Fr - World Tuberculosis Day-
O2k-Publications in the MiPMap "The molecular structure of the bd oxidase from M. tuberculosis provides the long-sought atomic framework for the design of highly specific drugs to target this respiratory enzyme"
  • Safarian S, Opel-Reading HK, Wu D, Mehdipour AR, Hards K, Harold LK, Radloff M, Stewart I, Welsch S, Hummer G, Cook GM, Krause KL, Michel H (2021) The cryo-EM structure of the bd oxidase from M. tuberculosis reveals a unique structural framework and enables rational drug design to combat TB. »Bioblast link«

  • Mar- 25 Sa
Registration deadline for »PDBEB 2023 Coimbra PT«

2023 Week 13
  • Mar- 27 Mo

  • Mar- 28 Tu
O2k-Open SupportInfo: improve Unusual stirrer bar rotation?

A “jumping” stirrer or a slow rotation of the stirrer bar may indicate a problem either with the stirrer itself or with the cleaning of the glass chamber.
- »Stirrer bar - technical support«
- communicated by »Baglivo Eleonora« and »Grings Mateus«

  • Mar- 29 We

  • Mar- 30 Th
How do you know if you damaged the mitochondria while preparing your sample?

The cytochrome c test – part of several SUIT protocols – is to check the integrity of the mitochondrial outer membrane.
- »Cytochrome c control efficiency«
- communicated by »Cecatto Cristiane« and »Timon-Gomez Alba«

  • Mar- 31 Fr

Feb 2023

2023 Week 5
  • Feb- 01 We

  • Feb- 02 Th

  • Feb- 03 Fr

  • Feb- 04 Sa

  • Feb- 05
»GRC Mitochondria in Health and Disease 2023 Lucca IT« Feb 05-10

2023 Week 6
  • Feb- 06 Mo
»GRC Mitochondria in Health and Disease 2023 Lucca IT« Feb 05-10

  • Feb- 07 Tu
O2k-brief repository Antitumoral properties of triterpenic acid functionalized gold nanoparticles arise from decreasing mitochondrial respiration
  • Mioc M, Mioc A, Racoviceanu R, Ghiulai R, Prodea A, Milan A, Barbu Tudoran L, Oprean C, Ivan V, Șoica C (2023) The antimelanoma biological assessment of triterpenic acid functionalized gold nanoparticles. »Bioblast link«

  • Feb- 08 We

  • Feb- 09 Th
FAT4BRAIN »FAT4BRAIN ESR Workshop«  Feb 09-10

  • Feb- 10 Fr

  • Feb- 11 Sa - International Day of Women and Girls in Science
Gentle Science “While progress has been made, women (particularly women of color) remain underrepresented in many scientific, engineering, and medical fields, and at many levels in education and career stages.”

Read this report for actionable recommendations to promote participation of females in STEM fields:

  • National Academies of Sciences, Engineering, and Medicine (2020) Promising practices for addressing the underrepresentation of women in science, engineering, and medicine: opening doors. The National Academies Press, Washington DC . »Bioblast link«

2023 Week 7
  • Feb- 13 Mo - International Epilepsy Day
O2k-Publications in the MiPMap "NAC [N-acetylcysteine] modulates neuronal excitability and the appearance or propagation of seizures through its antioxidant capacity and ability to reduce glycolytic/lactic metabolism in WAR [Wistar Audiogenic Rat – a genetic model of epilepsy]"
  • Dechandt CRP, Ferrari GD, Dos Santos JR, de Oliveira JAC, da Silva-Jr RMP, Cunha AOS, Garcia-Cairasco N, Alberici LC (2019) Energy metabolism and redox state in brains of wistar audiogenic rats, a genetic model of epilepsy. Front Neurol 10:1007. »Bioblast link«
from the O2k-Network »BR Ribeirao Preto Alberici LC«

  • Feb- 14 Tu
O2k-Workshops.png Oroboros O2k-Workshop on high-resolution respirometry (HRR) IOC 157 Los Angeles US Feb 14-16

  • Feb- 15 We - International Childhood Cancer Day
O2k-Publications in the MiPMap Mitochondrial respiratory dysfunction present in platelets from 10- to 11-year-old children with acute lymphoblastic leukemia
  • Lelcu T, Bînă AM, Dănilă MD, Popoiu CM, Aburel OM, Arghirescu ST, Borza C, Muntean DM (2021) Assessment of platelet mitochondrial respiration in a pediatric population: A pilot study in healthy children and children with acute lymphoblastic leukemia. »Bioblast link«
from the O2k-Network »RO Timisoara Muntean DM«

  • Feb- 16 Th
O2k-Open SupportInfo: improve Do you observe simultaneous fluctuations in the O2 slope neg. and the Peltier power?

This can indicate fluctuations in the room temperature or electrical supply. Controlling the temperature or changing the electrical supply may resolve this problem.
- »Troubleshooting«
- communicated by »Cecatto Cristiane« and »Schmitt Sabine«

  • Feb- 17 Fr
United Mitochondrial Disease Foundation 2023 UMDF Grant Cycle is now open for submissions through February 27th
- »Learn more«

  • Feb- 18 Sa
»BPS2023 San Diego US« Feb 18-22

  • Feb- 19 Su
09:30 (GMT-8) Erich Gnaiger presenting at BPS2023 San Diego US - Mitochondrial bioenergetics – a quantitative analytical and diagnostic approach
13:45 (GMT-8) Sabine Schmitt poster session at BPS2023 San Diego US - Contribution of fatty acid oxidation to respiratory control in brain mitochondria
2023 Week 8
  • Feb- 20 Mo
»BPS2023 San Diego US« Feb 18-22
13:45 (GMT-8) Erich Gnaiger poster session at at BPS2023 San Diego US - Respirometric substrate-uncoupler-inhibitor titration protocols for carbohydrate and fatty acid metabolism: from heart to brain mitochondria
Abstract submission deadline for »ATSPB 2023 Hall in Tirol AT«

  • Feb- 21 Tu

  • Feb- 22 We - World Encephalitis Day

  • Feb- 23 Th
O2k-Open SupportInfo: improve Add catalase when measuring Complex IV (CIV) activity in permeabilized fibers (pfi)

Pfi have a high content of iron-containing proteins, which accelerate the autoxidation of tetramethyl-p-phenylenediamine (TMPD) in the CIV assay. Catalase prevents the accumulation of H2O2 generated by this autoxidation.
- »Chemical Background« and »Complex IV«
- communicated by »Cecatto Cristiane« and »Schmitt Sabine«

  • Feb- 24 Fr

2023 Week 9
  • Feb- 27 Mo

O2k-Workshops.png 158th Oroboros O2k-Coaching Days - Basic Feb 27 - Mar 1

  • Feb- 28 Tu - Rare Disease Day

O2k-Workshops.png 158th Oroboros O2k-Coaching Days - Basic Feb 27 - Mar 1

Balbaisi 2022 BEC
Balbaisi and Stiban review the current knowledge on Barth Syndrome, a rare disease affecting the production of cardiolipin, the only phospholipid specific to mitochondria.

Jan 2023

2023 Week 1
  • Jan- 02 Mo

  • Jan- 03 Tu
Bombaca 2022 BEC
Bionenerg Commun 2022.20

Bombaça and Menna-Barreto discuss data on mitochondrial susceptibility and adaptative processes obtained by their group in the last 17 years.

  • Jan- 04 We

  • Jan- 05 Th
Early bird registration and abstract deadline for »BPS2023 San Diego US«

  • Jan- 06 Fr
2023 Week 2
  • Jan - 09 Mo
MitoGlobal Events For the upcoming 67th Annual Meeting of the Biophysical Society - Feb 18-22, we have four exhibitor area visitor passes available for our O2k-Network.
Get in touch if you are interested.

  • Jan- 10 Tu

  • Jan- 11 We
Which events are eligible for the O2k-Network Award?

Find out here: »O2k-Network Award: Eligible events«

  • Jan- 12 Th

  • Jan- 13 Fr

2023 Week 3
  • Jan- 16 Mo
Gnaiger 2020 BEC MitoPathways What is ROUTINE respiration?

ROUTINE respiration is the respiratory activity of living (non-permeabilized) cells in the physiological coupling state, which is controlled by cellular energy demand, energy turnover and the degree of coupling to phosphorylation.
- »Gnaiger 2020 BEC MitoPathways - Chapter 2.4« 
- communicated by »Schmitt Sabine«

  • Jan- 17 Tu

  • Jan- 18 We

  • Jan- 19 Th

  • Jan- 20 Fr

2023 Week 4
  • Jan- 23 Mo

  • Jan- 24 Tu - International Day of Education
Gnaiger 2021 BEC
Restricted access to academic research produces problems of social inequality in education: A re-evaluation of scientific communication and value of science is needed. Open access to research is essential, together with a bigger demand on quality control to ensure reproducibility of the results and prevent an avalanche of new publications that may go into oblivion.

  • Jan- 25 We

  • Jan- 26 Th

  • Jan- 27 Fr

2023 Week 5
  • Jan- 30 Mo
NextGen-O2k The NADH-Module of the NextGen-O2k incorporates a UV light and NADH-Sensors to enable the evaluation of the redox state of the NAD(P)H-pool

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  • Jan- 31 Tu

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