NextGen-O2k

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NextGen-O2k


NextGen-O2k         K-Regio MitoFit         O2k-Innovation         Houska-Award 2012         K-Regio MitoCom         Bioblast 2012


NextGen-O2k

The revolutionary all-in-one instrument to conquer mitochondrial disease

NextGen-O2k


NextGen-O2k: Horizon 2020 Framework Programme

  • EIC-SMEInst-2018-2020 — SME instrument phase 2, Grant Agreement No. 859770, Oroboros Instruments
  • Budget: € 2.35 Million
  • Duration: 24 months
  • Application: 2019-01-09; Pitch in Brussels: 2019-02-15; Granted: 2019-03-05; Start: 2019-06-01


About NextGenO2k

Top 10 reasons for Oroboros.jpg
Oroboros - as a driving force in mitochondrial physiology - extends the analytical and diagnostic power of high-resolution respirometry by integration of NADH- and Q-redox monitoring in the NextGen-O2k. We aim at establishing the Oroboros quality control management for dissemination to our worldwide O2k-Network laboratories. This will become an effective contribution to address the acute reproducibility crisis of scientific investigation. In the spirit of Open Science and global networking, we will enable data sharing across projects and institutions in an Open Access database on mitochondrial physiology and pathology, to resolve the inflation crisis and ultimately the value-impact crisis of present academic publication. This will support key developments in mitochondrial medicine. In addition, we expand our business to algal biotechnology and ecology with the photobiology module of the NextGen-O2k, widening our focus from medicine to environment and climate.
- a partner in the Oroboros Ecosystem

Aims

Respiration and photosynthesis
Form the O2k to the NextGen-O2k
Between 1992 and 1994, Oroboros Instruments released its core technology for high-resolution respirometry (HRR), the Oxygraph-2k or O2k, for real-time monitoring of oxygen consumption of mitochondria, tissues, and living cells. Dissolved oxygen is measured using high-resolution polarographic oxygen sensors. Its main advantages are the unique signal stability and resolution of oxygen. HRR is the core technology present in every single of the devices and it will remain the basis of the NextGen-O2k.
In 2012, the updated version of the O2k was launched, the O2k-FluoRespirometer. The integrated Smart Fluo-Sensors combine fluorescence excitation and detection of defined wavelengths that in combination with fluorophores allow for the measurement of additional mitochondrial parameters, such as membrane potential, H2O2 production, calcium concentration, and ATP production.
The unique software DatLab has been developed for data acquisition and analysis. DatLab allows the researcher to perform calibrations (including automatic barometric and temperature adjustments), background correction, experimental SUIT (substrate-uncoupler-inhibitor-titration) protocols and data analysis.


With the O2k core technology, developed exclusively by Oroboros for high-resolution measurement of oxygen flux, the NextGen-O2k was developed.


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O2k
High-resolution respirometry (HRR) and unique combinations of the O2k with





NextGen-O2k modules.jpg
NextGen-O2k
Extend high-resolution respirometry (HRR) to the all-in-one device
The NextGen-O2k is a MultiSensor all-in-one instrument for in-depth studies of (1) the mitochondrial role in human diseases, and (2) mitochondria and chloroplasts ability in regulating algal growth and metabolite production rates.
(+2) Controlled wavelengths and light intensity: PhotoBiology-Module
  • Save on experimental time, reagents and samples
  • Minimize errors and ensure reproducible and accurate results
  • Highest oxygen sensitivity (5 nM detection limit)
  • Exceptional resolution (wide oxygen concentration range, between 0 - 1,000 μM)
  • Robustness (>10 years lifespan)



Objectives

The overall goal of this Phase 2 project is to complete the technical and scientific development of the new NextGen-O2k and prepare it for market launch.
WP1
» Technical developments
WP2
» Scientific developments
WP3
» Documentation
WP4
» Exploitation
WP5
» Dissemination
WP6
» Project management

Coordinator

Oroboros Instruments GmbH

Network

Partners

Our partners WGT-Elektronik and HTech have been essential in the development of the NextGen-O2k.
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Early testers

The influence of KOLs and Early testers/adopters will be used to build awareness of NextGen-O2k among researchers, academic and industrial partners. We have confirmed relevant mitochondrial and algal biotechnology researchers who want to collaborate as early testers:


Biomedical research Algal research
Prof. Anthony Moore Prof. Tom Bibby
Prof. Massimo Zeviani, Dr. Carlo Viscomi, Prof. Mervyn Singer Dr. Tomas Morosinotto
Prof. Pablo Garcia-Roves Dr. Yagut Allahverdiyeva
Prof. Christos Chinopoulos Prof. Ted Packard
Dr. Anthony Molina Prof. Claire Remacle
Dr. Marten Szibor
Prof. Howard Jacobs
Dr. Alexander Karabatsiakis


NextGen-O2k events

 WhenWhere
OroDM01 Innsbruck AT2019-07-01
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Innsbruck AT, 2019 Jul 01-03 Oroboros Distributor Meeting, OroDM01.
NextGen-O2k SME2 coaching Orily Pratt part 12019-07-22
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Innsbruck AT, 2019 Jul 22-23 NextGen-O2k SME2 coaching Orily Pratt (part 1), NextGen-O2k SME2.
FEBS Workshop Ageing 2019 Innsbruck AT2019-09-09
FEBS
Innsbruck, AT. 2019 Sep 09-12, FEBS Workshop “Ageing and Regeneration”.
NextGen-O2k SME2 coaching Samia Kappe part 12019-09-12
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Innsbruck AT, 2019 Sep 10-11 NextGen-O2k SME2 coaching Samia Kappe (part 1), NextGen-O2k SME2.
MiPNet24.02 IOC141 Schroecken AT2019-09-23
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Schroecken AT, 2019 Sep 23-28 Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC141.
MiPNet24.13 IOC142 Fukuoka JP2019-10-03
O2k-Network
ASMRM & J-mit 2019, Fukuoka JP, 2019 Oct 03 Oroboros Lunch Seminar at ASMRM & J-mit 2019 Fukuoka JP - Explore the Mito-World! (IOC142).
ASMRM & J-mit 2019 Fukuoka JP2019-10-03
ASMRM LOGO.JPG
Fukuoka, JP, 2019 Oct 03-05. Joint ASMRM and J-mit Conference
NextGen-O2k SME2 coaching Orily Pratt part 22019-12-03
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Innsbruck AT, 2019 Dec 03-04 NextGen-O2k SME2 coaching Orily Pratt (part 2), NextGen-O2k SME2.
OroDM02 Schroecken AT2020
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Schroecken AT, Oroboros Distributor Meeting, OroDM02. Originally scheduled for 2020 Jul 06-07 - Postponing the ORODM02 appears to be unavoidable, more information will follow.
MiPNet25.10 Distributor engagement Tokyo JP2020-02-04
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Tokyo,JP, 2020 Feb 4-7. On-site O2k-Workshop on High-Resolution Respirometry and partner distribution engagement.
36th Congress Czech Nutrition Society 2020 Hradec Kralove CZ2020-09-10Hradec Kralove, CZ, 2020 Sep 10-12 . 36th annual international congress of Czech Nutrition Society
NextGen-O2k Launching event 2021 Innsbruck AT2021-05-03
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Innsbruck AT, 2021 May 3-8. NextGen-O2k Launching event, NextGen-O2k SME2.


NextGen-O2k dissemination

NextGen-O2k press releases


Gentle Science

In addition to O2k-Workshops and on-site training, Oroboros will disseminate the not-for-profit initiatives on Mitochondrial Physiology and Bioenergetics Science Camps, specialized Mitochondrial Physiology Conferences, and Preprint publications:
» Oroboros is a key player in support of the COST Action MitoEAGLE
» MitoGlobal - the world-wide platform for societies and organizations supporting mitochondrial research and medicine
» MitoEAGLE with cooperation by Oroboros launched MitoFit Preprint Archives - the preprint server for mitochondrial physiology and bioenergetics
» A vision on preprints
» Mitochondrial respiratory states and rates : >530 coauthors join forces towards harmonization on nomenclature
» Gentle Science - recognizes the responsibility of the scientific community - for the quality of science, the quality of life in science, and its mission


MitoEAGLE Q-workshop

MitoFit



Support

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Supported by project NextGen-O2k which has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 859770.

MitoPedia: NextGen-O2k

» NextGen-O2k«
TermAbbreviationDescription
Hydrogen peroxideH2O2
Hydrogen peroxide
Hydrogen peroxide, H2O2 or dihydrogen dioxide, is one of several reactive oxygen intermediates generally referred to as reactive oxygen species (ROS). It is formed in various enzyme-catalyzed reactions (e.g., superoxide dismutase) with the potential to damage cellular molecules and structures. H2O2 is dismutated by catalase to water and oxygen. H2O2 is produced as a signaling molecule in aerobic metabolism and passes membranes more easily compared to other ROS.
Mitochondrial membrane potentialmtMP, Δψ [V]The mitochondrial membrane potential, mtMP, is the electric part of the protonmotive force, ΔpH+.

Δψ = ΔpH+ - ΔµH+ / F

mtMP or Δψ is the potential difference across the inner mitochondrial (mt) membrane, expressed in the electric unit of volt [V]. Electric force of the mitochondrial membrane potential is the electric energy change per ‘motive’ electron or per electron moved across the transmembrane potential difference, with the number of ‘motive’ electrons expressed in the unit coulomb [C].
NextGen-O2k
NextGen-O2k InstrumentNextGen-O2k Instrument
NextGen-O2k Technical developments
Oxygen kineticsOxygen kinetics describes the dependence of respiration of isolated mitochondria or cells on oxygen partial pressure. Frequently, a strictly hyperbolic kinetics is observed, with two parameters, the oxygen pressure at half-maximum flux, p50, and maximum flux, Jmax. The p50 is in the range of 0.2 to 0.8 kPa for cytochrome c oxidase, isolated mitochondria and small cells, strongly dependent on Jmax and coupling state.
PhotoBiologyPBPhotoBiology is the scientific study of the beneficial or harmful effects of light, understood as non-ionizing radiation (i.e. ultraviolet, visible and infrared radiation), on living organisms. It includes topics such as the study of photosynthesis, photochemistry, photophysics, photomorphogenesis, vision, bioluminescence, circadian rhythms and photodynamic therapy. Non-ionizing (or non-ionising) radiation is any type of electromagnetic radiation that does not carry enough energy per quantum (photon energy below 10 eV) to completely remove an electron from an atom or molecule. When photons contact molecules, the molecules can absorb the photon energy and become excited, reacting with surrounding molecules and stimulating "photochemical" and "photophysical" changes.
Q redox stateQQ redox state


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MitoPedia:NextGen-O2k