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OroboPedia topics

TermDescription
10th European Algae Industry Summit 2020 Reykjavik IS
10th Int CeBiTec Research Conference 2021 Bielefeld DE
16th Chinese Biophysics Congress 2018 Chengdu CH
46th ISOBM Congress 2019 Athens GR
4th edition Metabolism & Cancer 2021 Virtual
6th Biannual Meeting on Mitochondria Apoptosis & Cancer 2019 Prague CZ
7th World Congress on Targeting Microbiota 2019 Krakow PL
8th SMRM and Mitochondria-Metabolism Network Meeting 2020 Pune IN
ALGAEUROPE 2018 Amsterdam NL
AR Buenos Aires Boveris A
ASMRM & J-mit 2019 Fukuoka JP
ASMRM 2017 Xian CN
ASMRM 2018 Busan KR
ASMRM 2021 Singapore SG
AT Graz Graier W
AT Graz Zechner R
AT Innsbruck Burgstaller W
AT Innsbruck Burtscher M
AT Innsbruck Gnaiger E
AT Innsbruck HTech
AT Innsbruck Jansen-Duerr P
AT Innsbruck MitoFit
AT Innsbruck Oroboros
AT Innsbruck Schneeberger S
AT Kolsass WGT
AT Salzburg Breitenbach M
AT Salzburg Sperl W
AT Vienna Bittner RE
AT Vienna Kozlov AV
ATPAdenosine triphosphate is a nucleotid and functions as the major carrier of chemical energy in the cells. As it transfers its energy to other molecules, it looses its terminal phosphate group and becomes adenosine diphosphate (ADP).
AU Chermside Molenaar P
AU Clayton St John J
AU Melbourne Bishop DJ
AU Melbourne Bond S
AU Melbourne Coughlan M
AU Melbourne Hardee JP
AU Melbourne Thouas G
AU Melbourne Trounce IA
AU Melbourne White C
AU Perth Filipovska A
AU Queensland Neuzil J
AU Southport Peart J
AU St Lucia Coombes J
AU Sydney Ballard JW
AU Sydney Griffith SC
AU Sydney James D
AU Sydney Philp A
AU Sydney Stocker R
Abbasi 2018 J R Soc Med
AlgaEurope 2020 Virtual Event
All O2k-Workshop
Allahverdiyeva-Rinne Yagut
Amplex UltraRedAmplex® UltraRed (AmR) is used as an extrinsic fluorophore for measurement of hydrogen peroxide production (ROS) by cells or mitochondrial preparations. The reaction of H2O2 and AmR is catalyzed by horseradish peroxidase to produce the red fluorescent compound resorufin (excitation wavelength 563 nm, emission 587 nm; the fluorescent product according to the supplier is called UltroxRed in the case of Amplex® UltraRed which has a very similar structure to resorufin). The change of emitted fluorescence intensity is directly proportional to the concentration of H2O2 added, whereby the H2O2 is consumed.
Attached cellsMany cell types are grown in culture as attached cells, such as endothelial or neuronal cells in a monolayer.
Avasthi 2018 eLife
BE Gent Braeckman BP
BE Leuven Spinazzi M
BE Leuven Vermeersch P
BE Liege Sluse F
BE Liege Votion DM
BEC 2020.1 doi10.26124bec2020-0001.v1
BEC authors
BEC editors
BEC formats
BEC reviewers
BPS19 2019 Baltimore US
BR Brasilia De Bem AF
BR Campinas Carneiro EM
BR Campinas Vercesi AE
BR Criciuma Dal-Pizzol F
BR Criciuma Muller AP
BR Florianapolis Latini A
BR Jaboticabal Oliveira MT
BR Manaus Val AL
BR Porto Alegre Klamt F
BR Porto Alegre Souza DOG
BR Ribeirao Preto Alberici LC
BR Rio de Janeiro Da Poian AT
BR Rio de Janeiro Galina A
BR Rio de Janeiro Institute Biomedical Chemistry
BR Rio de Janeiro Moura AS
BR Rio de Janeiro Oliveira MF
BR Rio de Janeiro Paes MC
BR Rio de Janeiro Rumjanek FD
BR Rio de Janeiro Vieyra A
BR Santa Maria Soares FA
BR Sao Gabriel Franco JL
BR Sao Paulo Bresciani Martins de Andrade P
BR Sao Paulo Ferreira JCB
BR Sao Paulo Festuccia W
BR Sao Paulo Kowaltowski AJ
BR Sao Paulo Silber AM
Beno Marija
Berg 2016 Science
Berg 2017 Science
Bhalla 2016 Mol Biol Cell
Bioblast 2012
Bioblast 2012: Registration and accommodation
Bioblast 2012: Scientific Committee
Bioblast:About
Bioenerg Commun
Bioenergetics Communications Policies
Biological contaminationBiological contamination may be caused by microbial growth in the O2k-Chamber or in the experimental medium.
Bolivia-Mount Chacaltaya 2012
Bourne 2017 PLoS Comput Biol
Bove-Fenderson 2018 JBMR Plus
Brown 2020 MitoFit Preprint Arch
CA Antigonish Kane DA
CA Calgary Kinesiology Univ Calgary
CA Calgary Shearer J
CA Chalk River Paterson L
CA Charlottetown Kamunde C
CA Edmonton Lemieux H
CA Edmonton Zaugg M
CA Guelph Ballantyne JS
CA Guelph Holloway GP
CA Hamilton Scott GR
CA Hamilton Singh G
CA Hamilton Tarnopolsky MA
CA London Staples JF
CA Moncton Boudreau L
CA Moncton Hebert-Chatelain E
CA Montreal Bergdahl A
CA Montreal Breton S
CA Montreal Hepple RT
CA Ottawa Darveau CA
CA Ottawa Harper ME
CA Ottawa Pamenter M
CA Quebec Soliz J
CA Rimouski Blier PU
CA Saint John Pulinilkunnil T
CA Toronto Perry CG
CA Vancouver Boushel RC
CA Vancouver Richards JG
CA Waterloo McDonald AE
CA Winnipeg Banerji V
CA Winnipeg Fernyhough P
CA Winnipeg Treberg JR
CH Basel Balavenkatraman KK
CH Basel Eckert A
CH Basel Kraehenbuehl S
CH Basel Uteng M
CH Bern Djafarzadeh S
CH Bern Longnus SL
CH Bern Nuoffer JM
CH Lausanne Auwerx J
CH Lausanne Bagni C
CH Lausanne Canto C
CH Lausanne EPFL
CH Lausanne Place N
CH Lausanne Sandi C
CH Zurich Gassmann M
CH Zurich Lundby C
CH Zurich University of Zurich Physiology
CH Zurich Wallimann T
CL Santiago Regueira T
CN Baoding Cao X
CN Baoding Liu F
CN Beijing Chen Q
CN Beijing Huawei
CN Beijing Li C
CN Beijing Liu L
CN Beijing Qin Y
CN Beijing Wang X
CN Beijing Yuan Z
CN Chongqing Huang J
CN Chongqing Zhu Z
CN Dalian Zhan L
CN Guangzhou Liu J
CN Guangzhou Zhang X
CN Hangzhou Zhu W
CN Hongkong Huawei
CN Jinan Wang C
CN Macao Huawei
CN Nanjing Gan Z
CN Shanghai Liu T
CN Shanghai Yi X
CN Shanghai Zenda
CN Tianjin Zhang Y
CN Tianjin Zhao H
CN Wuhan Huang K
CN Xiamen Lin SC
CN Zengzhou Wu S
CN Zunyi Zhou S
CO Medellin Gomez LA
CSH Asia 2017 Suzhou CN
CSLMB 2018 Shanghai CN
CU Havana Pardo Andreu GL
CZ Ceske Budejovice Zikova A
CZ Hradec Kralove Cervinkova Z
CZ Olomouc Modriansky M
CZ Pardubice Rousar T
CZ Pilsen Kuncova J
CZ Prague Bioenergetics
CZ Prague Fisar Z
CZ Prague Houstek J
CZ Prague Jezek P
CZ Prague Kalous M
CZ Prague Kopecky J
CZ Prague Krajcova A
CZ Prague Neuzil J
CZ Prague Pichova A
CZ Prague Zeman J
CalciumCa2+ is a major signaling molecule in both prokaryotes and eukaryotes. Its cytoplasmic concentration is tightly regulated by transporters in the plasma membrane and in the membranes of various organelles. For this purpose, it is either extruded from the cell through exchangers and pumps or stored in organelles such as the endoplasmic reticulum and the mitochondria. Changes in the concentration of the cation regulate numerous enzymes including many involved in ATP utilizing and in ATP generating pathways and thus ultimately control metabolic activity of mitochondria and of the entire cell. Measuring changes in Ca2+ levels is thus of considerable interest in the context of high-resolution respirometry.
Calcium GreenCalcium Green denotes a family of extrinsic fluorophores applied for measurement of Ca2+ concentration.
Callaway 2013 Nature
Canonical Carol Canon O
Cardoso 2021 BEC MgG
Cardoso 2021 MitoFit MgG
Cardoso Luiza HD
Career Event - FH Campus 2021 Wien AT
Cecatto 2021 CaG
Chabi 2019 MitoFit Preprint Arch EA
Chalmers 2016 F1000Research
Chawla 2017 Nature
Chinese Academy of pathophysiology, endocrinology and metabolism Specialized Committee Conference 2018 Tianjin CN
Chinopoulos Christos
Chlamy 2021 Ile des Embiez FR
ChlororespirationIn chlororespiration oxygen is consumed by a putative respiratory electron transfer system (ETS) within the thylakoid membrane of the chloroplasts and ATP is produced. It is a process that involves the interaction with the photosynthetic ETS in which NAD(P)H dehydrogenase transfers electrons to oxygen with the assistance of the photosynthetic plastoquinone (PQ), which acts as a non-photochemical redox carrier. Initially described in the unicellular alga Chlamydomonas reindhartdii, chlororespiration was highly disputed for years until the discovery of a NAD(P)H-dehydrogenase (NDH) complex (plastidic encoded) and plastid terminal oxidase (PTOX) (nuclear encoded) in higher-plant chloroplasts. PTOX is homologous to the plant mitochondrial alternative oxidase and has the role of preventing the over-reduction of the PQ pool while the NDH complexes provide a gateway for the electrons to form the ETS and consume oxygen. As a result of this process there is a cyclic electron flow around Photosystem I (PSI) that is activated under stress conditions acting as a photoprotection mechanism and could be involved in protecting against oxidative stress.
Citron 2015 Proc Natl Acad Sci U S A
Closed chamberThe O2k-chamber can be used as a closed system or open system. Gas bubbles must be avoided.
Cobb 2017 PeerJ Preprints
Committee 2018 COPE Discussion Document
Cover-Slip\black
Cover-Slip black.JPG
A Cover-Slip should be placed on top of the O2k-Stopper to minimize contamination and evaporation of liquid extruding from the capillary of the stopper. The Cover-Slips do not exert any direct effect on oxygen backdiffusion into the O2k-chamber. Use the the Cover-Slip\black to avoid light penetration and disturbance of fluorescence signals and generally for optical measurements in the O2k.
Credit card payment
Crispim 2019 MitoFit Preprint Arch EA
CureMILS
Custom-made stoppersStoppers can be custom-made for applications with user-specific sensors according to customer specifications.
Cyclic voltammetryCyclic voltammetry (CV) is a type of electrochemical measurement which is applied with the Q-Module as quality control to

(1) determine the oxidation and reduction peak potentials of Coenzyme Q in the specific experimental condition, (2) check the quality of the Q-Sensor, and (3) test the interference of chemicals used in the HRR assay with the Q-Sensor. In CV, the Q-Sensor with the three-electrode system is used to obtain information about the analyte (CoQ) by measuring the current (I) as the electric potential (V) between two of the electrodes is varied. In CV the electric potential between the glassy carbon (GC) and the Ag/AgCl reference electrode changes linearly versus time in cyclical phases, while the current is detected between GC and platinum electrode (Pt). The detected current is plotted versus the applied voltage to obtain the typical cyclic voltammogram trace (Figure 1). The presence of substances that are oxidized/reduced will result in current between GC and Pt, which can be seen as characteristic peaks in the voltammogram at a defined potential. The oxidation or the reduction peak potential values are used to set the GC (integrated into the Q-Sensor) for a separate experiment to measure the Q redox state of a biological sample. The oxidation and reduction peak potentials can be influenced by 1) the respiration medium, 2) the type of CoQ, 3) the polarization window, 4) the scan speed, 5) the number of cycles, 6) the concentration of the analyte (CoQ), and 7) the initial polarization voltage. <be>

-See: MiPNet24.12 NextGen-O2k: Q-Module.
DE Berlin Boschmann M
DE Biberach Kussmaul L
DE Bonn Kunz WS
DE Bonn Pfeifer A
DE Bremerhaven Mark FC
DE Cologne Aging Research
DE Cologne Antebi A
DE Cologne Larsson NG
DE Cologne Trifunovic A
DE Duesseldorf Grieshaber MK
DE Duesseldorf Haendeler J
DE Duesseldorf IUF
DE Duesseldorf Ibing W
DE Duesseldorf Roden M
DE Duesseldorf Westenfeld R
DE Essen Bienholz A
DE Essen De Groot H
DE Essen Ferenz K
DE Essen Ferenz KB
DE Essen Gedik N
DE Essen Kirsch M
DE Frankfurt Droese S
DE Frankfurt Eckert GP
DE Frankfurt Leeuw T
DE Frankfurt Osiewacz HD
DE Frankfurt Sanofi
DE Frankfurt Schmoll D
DE Frankfurt Wittig I
DE Freiburg Pfanner N
DE Freiburg Schuele R
DE Freising Klingenspor M
DE Giessen Eckert GP
DE Giessen Rohrbach S
DE Giessen Weissmann N
DE Goettingen Dennerlein S
DE Goettingen Krischek C
DE Goettingen Moerlein D
DE Goettingen Mueller M
DE Goettingen Wicke M
DE Hamburg Singer D
DE Hannover Hildebrandt T
DE Heidelberg Labeit S
DE Heidelberg Mairbaeurl H
DE Heidelberg Teleman A
DE Hottersheim Kabiri M
DE Jena Szibor M
DE Kiel Herdegen T
DE Koblenz Ortmann C
DE Konstanz Brdiczka D
DE Kronshagen Grams B
DE Leipzig Maskow T
DE Leipzig Mueller A
DE Leipzig UFZ Environmental Research
DE Magdeburg Debska-Vielhaber G
DE Magdeburg Gellerich FN
DE Magdeburg Klinik Neurologie
DE Magdeburg Schild L
DE Magdeburg Schoenfeld P
DE Mainz Methner A
DE Munich Elstner M
DE Munich Jastroch M
DE Munich Perocchi F
DE Munich Zischka H
DE Nuthetal Klaus S
DE Regensburg Reichold M
DE Regensburg Renner-Sattler K
DE Rostock Sokolova I
DE Seewiesen Casagrande S
DE Tuebingen Weigert C
DE Ulm Gumpp A
DE Ulm Karabatsiakis A
DE Ulm Radermacher P
DE Wuerzburg Maack C
DK Aarhus Boetker HE
DK Aarhus Fago A
DK Copenhagen Christiansen M
DK Copenhagen Dela F
DK Copenhagen Gerhart-Hines Z
DK Copenhagen Larsen A
DK Copenhagen Larsen S
DK Copenhagen Lundby C
DK Copenhagen Pilegaard H
DK Copenhagen Quistorff B
DK Malov Hey-Mogensen M
Da Silva 2018 Med J Armed Forces India
DatLab
DatLab
DatLab is the O2k-Software for Data Acquisition & Analysis, specifically developed for high-resolution respirometry with the O2k-FluoRespirometer.

The newest DatLab version is DatLab 7.4, included in the O2k-FluoRespirometer.


The minimum computer requirements are Intel-Core-2 or equivalent CPU, 2GB RAM and Windows XP. However, we recommend Intel i5 or equivalent CPU, 4GB RAM, Windows 10 and SSD. For the proper display of DatLab on your computer, please make sure the “Language settings” are set to English.
DatLab 2DatLab 2 (DL2) is a MS-DOS programe. DL2 is still used for analysis of oxygen kinetics, after exporting files recorded in recent DatLab versions. A user-friendly O2-kinetics module is in preparation (DL8).
DatLab-Upgrading to DatLab 6DatLab-Upgrading to DatLab 6: including free follow-up updates for DatLab 6 for the next two years
DatLab-Upgrading\4.x-5.2DatLab-Upgrading\4.1-5.2: Upgrading DatLab 4.x to 5.2, incl. O2k-Manual, with free follow-up updates of DatLab 5.2. Discontinued: see higher DatLab version.
Desjardins-Proulx 2013 PLOS Biol
Development MitoFit proficiency test
Di Marcello 2019 MitoFit Preprint Arch EA
Di Marcello Marco
Die Kraftwerke der Zellen
Different O2 fluxes in left and right chamberWhat are potential causes for different O2 fluxes in the left and right chamber?
DithioniteDithionite Na2S2O4 is the 'zero oxygen solution powder' used for calibration of oxygen sensors at zero oxygen, or for stepwise reduction of oxygen concentrations in instrumental O2 background tests. It is not recommended to use dithionite in experiments with biological samples or several multisensor approaches, for these see Setting the oxygen concentration.
Doerrier 2019 MitoFit Preprint Arch EA
Doerrier Carolina
EE Tallinn Kaambre T
EE Tallinn Saks VA
EE Tartu Paju K
EE Tartu Seppet EK
EG Cairo Ali SS
ES Barcelona Bosch M
ES Barcelona Claret M
ES Barcelona Garcia-Roves PM
ES Barcelona Gomis R
ES Barcelona IDIBAPS Hospital Clinic
ES Barcelona Moren C
ES Barcelona Zorzano A
ES CN Las Palmas Calbet JAL
ES Granada Acuna-Castroviejo D
ES L'Hospitalet Bermudez MJ
ES Lleida Boada J
ES Madrid Cadenas S
ES Madrid Enriquez JA
ES Madrid Garesse R
ES Malaga Medina MA
ES Santiago De Compostela Mendez-Alvarez E
ES Tarragona Arola L
ES Valencia Casado Pinna M
ES Valencia Gomez Cabrera
ES Valencia Meseguer S
ES Zaragoza Ruiz-Pesini E
ESP2021 Salzburg AT
Electrolyte\Reference-Electrode
Electrolyte Reference-Electrode.jpg
Electrolyte\Reference-Electrode for Reference-Electrode\2.4 mm
Electronic-TIP2k Upgrading\O2k-Main Unit Series A-DElectronic-TIP2k Upgrading\O2k-Main Unit Series A-D - Former Product : not required for O2k-Core, the O2k-Main Unit has to be returned to the OROBOROS workshop.
Electronic-TIP2k Upgrading\O2k-Main Unit Series EElectronic-TIP2k Upgrading\O2k-Main Unit Series E - Former Series : not required for O2k-Core, free of charge for Series E in conjunction with the purchase of the TIP2k-Module, the O2k-Main Unit has to be returned to the OROBOROS workshop.
Enable DL-Protocol editingEnable DL-Protocol editing is a novel function of DatLab 7.4 offering a new feature in DL-Protocols: flexibility. Fixed sequences of events and marks can be changed (Skip/Added) in a SUIT protocol by the user. Moreover, the text, instructions, concentrations and titration volumes of injections in a specific DL-Protocol can be edited and saved as user-specific DL-Protocol [File]\Export\DL-Protocol User (*.DLPU). To enable it, under the 'Protocols' tab in the menu, select the option 'Enable DL-Protocol editing', and then select the plot in which the marks will be set (e.g., O2 flux per V). Select the 'Overview' window, where you will be able to edit events and marks names, definition/state, final concentration and titration volumes, as well as select a mark as 'multi' for multiple titration steps, skip a mark, or add a new event or mark. After saving, export a DL-Protocol User (DLPU) and load it before running the next experiments. If users of DatLab versions older than DatLab 7.4 wish to alter the nature of the chemicals used or the sequence of injections, we ask them to contact the O2k-Technical Support.

For more information:

PlayVideo.jpg Export DL-Protocol User (*.DLPU)
Expert/inn/en-Workshop Medizintechnik Innsbruck AT
Export DL-Protocol User (*.DLPU)Export DL-Protocol User (*.DLPU) is a function of DatLab (available from version 7.4 onwards) that enables the export of user specific protocols (DL-Protocol User (*.DLPU)) to the SUIT protocol folder from which they can be uploaded for subsequent measurements. *.DLPU are generated by editing an existing SUIT protocol according to the user's research question (Enable DL-Protocol editing). The modified user-specific DL-Protocol needs to be saved before it can be exported.
Text file - DatLab.png


For more information: PlayVideo.jpg Export DL-Protocol User (*.DLPU)







Extended abstractsIn the context of MiPevents and MitoEAGLE events, extended abstracts are accepted for preprint publication in MitoFit Preprints upon evaluation by MitoEAGLE MC members. Publishing extended abstracts with MitoFit Preprints does not preclude later full journal publication, but will make your work fully citable, by assigning each manuscript a unique DOI number, and facilitate discovery and feedback.
FAT4BRAIN
FEBS 2019 Krakow PL
FEMtech Internship for students
FI Helsinki Jacobs HT
FI Helsinki Mervaala E
FI Helsinki Pirinen E
FI Helsinki Wikstroem M
FI Jyväskylä Kainulainen H
FI Oulu Kastaniotis A
FI Tampere Dufour E
FI Turku Nikinmaa M
FR Angers Andriantsitohaina R
FR Angers Gueguen N
FR Aubiere Sirvent P
FR Bordeau Di Rago JP
FR Bordeaux Devin A
FR Bordeaux Mourier A
FR Bordeaux Rossignol R
FR Dijon Leloup C
FR Evry Tardo-Dino PE
FR Fort de France Neviere R
FR Grenoble Saks VA
FR Grenoble Schlattner U
FR La Rochelle Rosenfeld E
FR Lille Boutry M
FR Lille Duez H
FR Lille Lancel Steve
FR Lille Vienne JC
FR Lyon Ovize M
FR Marseille Bioenergetics
FR Marseille Brasseur G
FR Marseille Denis M
FR Marseille Gregori G
FR Montpellier Chabi B
FR Montpellier Fauconnier J
FR Montpellier Prouteau-Angebault C
FR Montpellier Salmon JM
FR Paris Bouillaud F
FR Pessac Pasdois P
FR Plouzane Salin K
FR Saint Gilles Lefaucheur L
FR Strasbourg Blanc S
FR Strasbourg Zoll J
FR Suresnes Sabatini M
FR Toulouse Casteilla L
FR Toulouse Dray C
FR Toulouse Letellier T
FR Tours Dumas JF
FR Villeurbanne Romestaing C
Fatty acid oxidationFatty acid oxidation (β-oxidation) is a multi-step process by which fatty acids are broken down to generate acetyl-CoA, NADH and FADH2 for further energy transformation in the mitochondrial matrix. Whereas NADH is the substrate of CI, FADH2 is the substrate of Electron-transferring flavoprotein complex (CETF) which is localized on the matrix face of the mtIM, and supplies electrons from FADH2 to CoQ2. Before the mitochondrial transport for ß-oxidation, fatty acids (short-chain with 1-6, medium-chain with 7–12, long-chain with >12 carbon atoms) are activated by fatty acyl-CoA synthases (thiokinases) in the cytosol. For the mitochondrial transport of long-chain fatty acids the mtOM-enzyme carnitine palmitoyltransferase I (CPT-1; rate-limiting step in FAO) is required which generates an acyl-carnitine intermediate from acyl-CoA and carnitine. In the next step, an integral mtIM protein carnitine-acylcarnitine translocase (CACT) catalyzes the entrance of acyl-carnitines into the mitochondrial matrix in exchange for free carnitines. In the inner side of the mtIM, another enzyme carnitine palmitoyltransferase 2 (CPT-2) converts the acyl-carnitines to carnitine and acyl-CoAs, which undergo ß-oxidation in the mitochondrial matrix. Short- and medium-chain fatty acids do not require the carnitine shuttle for mitochondrial transport. Octanoate, but not palmitate, (eight- and 16-carbon saturated fatty acids) may pass the mt-membranes, but both are frequently supplied to mt-preparations in the activated form of octanoylcarnitine or palmitoylcarnitine.
Filter Set AmR
Filter Set AmR.JPG
Filter Set AmR: Set of filters for the determination of H2O2 production with Amplex UltraRed. These filters should be used together with Fluorescence-Sensor Green. The filter set consists of 6 LED filters (round) and 6 photodiode filters (rectangular).
Filter Set MgG / CaG
Filter Set MgG CaG.JPG
Filter set MgG / CaG: Set of filters for the determination of concentraions of Mg2+ or Ca2+ with the fluorophores Magnesium green and Calcium green, respectively. These filters should be used together with Fluorescence-Sensor Blue or Smart Fluo-Sensor Blue. The filter set consists of 6 LED filters (round) and 6 photodiode filters (rectangular).
Filter Set Saf
Filter Set Saf.JPG
Filter set Saf: Set of filters for the (qualitative) determination of mitochondrial membrane potential with Safranin. These filters should be used together with Fluorescence-Sensor Blue or Smart Fluo-Sensor Blue. The filter set consists of 6 LED filters (round) and 6 photodiode filters (rectangular).
Filter-Cap
Filter-Cap.JPG
Filter-Cap: O2k-Fluo LED2-Module (O2k-Series D to G) sensors (Fluorescence-Sensor Green and Fluorescence-Sensor Blue) and O2k-FluoRespirometer (O2k-Series H to I) sensors (Smart Fluo-Sensor Green and Smart Fluo-Sensor Blue) are equipped with a removable Filter-Cap for exchange of optical filters for the optical pathways from the LED to the sample and from the sample to the photodiode.
Fluorescence-Control Unit
Fluorescence-Control Unit lettered.jpg
Fluorescence-Control Unit with O2k-Front Fixation, Current-Control (O2k-Chamber A and B) for regulation of light intensity of the LED in the fluorescence sensors. This item is a standard component of the O2k-Fluorescence LED2-Module.
Fluorescence-Sensor Blue
Fluorescence-Sensor Green.JPG
Fluorescence-Sensor Blue: excitation LED 465 nm (dominant wavelength), photodiode, Filter-Cap equipped with Filter Set Saf for measurement of mitochondrial membrane potential with Safranin when delivered. Filter sets for Magnesium green® / Calcium green® measurements are included.
Fluorescence-Sensor Green
Fluorescence-Sensor Green.JPG
Fluorescence-Sensor Green: excitation LED 525 nm (dominant wavelength), photodiode, Filter-Cap equipped with Filter Set AmR for Amplex UltraRed measurements when delivered.
Fluorescence-Service Box
Fluorescence-Service Box.JPG
Fluorescence-Service Box: for storage and shipment of theO2k-Fluo LED2-Module.
Forceps for membrane application
Forcep for membrane application.jpg
Forceps for membrane application: for OroboPOS and ISE membrane application; do not use for tissue preparation.
Forceps\stainless Steel\angular Tip\fine
Forcep for tissue preparation angular tip.jpg
Forceps\stainless Steel\angular Tip\fine: for tissue preparation, stainless steel. Two pairs are used particularly for muscle fiber separation.
Forceps\stainless Steel\rounded Tip\sharp
Forcep for tissue preparation rounded tip.jpg
Forceps\stainless Steel\rounded Tip\sharp: for tissue preparation, stainless steel, antimagnetic. One pair is recommended for placing the tissue sample onto the microbalance and for handling in combination with Forceps\stainless Steel\straight Tip\sharp.
Forceps\stainless Steel\straight Tip\sharp
Forcep for tissue preparation straight tip.jpg
Forceps\stainless Steel\straight Tip\sharp: for tissue preparation, stainless steel, antimagnetic. One pair is recommended for insertion of the sample into the O2k-chamber and for handling in combination with Forceps\stainless Steel\rounded Tip\sharp.
Fraser 2018 Nature
GDPR
GRC Meeting on Organelles including Mitochondria 2019 West Dover US
GRC on Mitochondrial Dynamics and Signaling 2019 Ventura US
GRS on Mitochondria & Chloroplasts 2022 West Dover US
GRS on Organellar Channels and Transporters 2023 Castelldefels ES
GainThe gain is an amplification factor applied to an input signal to increase the output signal.
Garcia-Roves Pablo Miguel
Ginsparg 2017 arXiv
Gnaiger 2012 Mitochondr Physiol Network Bioblast 2012
Gnaiger 2014 MitoPathways
Gnaiger 2019 MitoFit Preprints
Gnaiger 2019 MitoFit Preprints Editorial
Gnaiger 2020 BEC MitoPathways
Gnaiger 2020 MitoFit x
Gnaiger 2021 MitoFit BCA
Gnaiger 2021 X-mass Carol
Gnaiger Erich
Gnaiger IOC62-Introduction
Gonçalves 2019 Mitofit Preprint Arch EA
Gradl P
Greenland Expedition CMRC 2004
Gross 2018 Neurology
Gurkina A
HR Split Ljubkovic M
HU Budapest Chinopoulos C
HU Budapest Semmelweis Univ
HU Budapest Tretter L
HU Debrecen Virag L
HU Szeged Boros M
Haider Markus
Hassan 2020 MitoFit Preprint Arch
Hatch 1998 JAMA
High signal at zero oxygenA high signal at zero oxygen may be observed during zero calibration. The following instructions show how to further localize and possibly solve this technical problem.
Holzner 2019 MitoFit Preprint Arch EA
Houska Award 2012
Huete-Ortega 2020 MitoFit Preprint Arch EA
Huete-Ortega Maria
Hydrogen peroxide
Hydrogen peroxide
Hydrogen peroxide, H2O2 or dihydrogen dioxide, is one of several reactive oxygen intermediates generally referred to as reactive oxygen species (ROS). It is formed in various enzyme-catalyzed reactions (e.g., superoxide dismutase) with the potential to damage cellular molecules and structures. H2O2 is dismutated by catalase to water and oxygen. H2O2 is produced as a signaling molecule in aerobic metabolism and passes membranes more easily compared to other ROS.
Hydrogenion fluxVolume-specific hydrogenion flux or H+ flux is measured in a closed system as the time derivative of H+ concentration, expressed in units [pmol·s-1·mL-1]. H+ flux can be measured in an open system at steady state, when any acidification of the medium is compensated by external supply of an equivalent amount of base. The extracellular acidification rate (ECAR) is the change of pH in the incubation medium over time, which is zero at steady state. Volume-specific H+ flux is comparable to volume-specific oxygen flux [pmol·s-1·mL-1], which is the (negative) time derivative of oxygen concentration measured in a closed system, corrected for instrumental and chemical background. pH is the negative logarithm of hydrogen ion activity. Therefore, ECAR is of interest in relation to acidification issues in the incubation buffer or culture medium. The physiologically relevant metabolic H+ flux, however, must not be confused with ECAR.
IE Dublin Miinalainen I
IE Dublin O Gorman D
IE Dublin Porter RK
IL Rishon Le Zion Hachmo Y
IN Haldia Chakrabarti S
IN Hyderabad Thangaraj K
IN Lucknow Gayen JR
IN Mumbai Kolthur-Seetharam U
IN New Delhi Mukhopadhyay A
IN Thiruvananthapuram Gopala S
IN Varanasi Dash D
IOC Innsbruck
IOC Schroecken
IOC recommended reading
IOC student scholarship
IOC05
IOC10
IOC13
IOC16
IOC33
IOC42
IOC43 Montevideo UY 2007
IOC48
IOC62
IPC2021 Puerto Varas CL
ISAP 2021 Virtual
ISE Package 1 TPP or Ca
ISE Package 1 TPP or Ca.JPG
O2k-TPP+ and Ca2+ ISE\1 Chamber: ISE-Package for 1 TPP+ and Ca2+ electrode.
ISE-Ca2+ Membranes
Ca2+ membranes.jpg
ISE-Ca2+ Membranes: PVC, 4 mm diameter, box of 5 membranes. To be used with the O2k-TPP+ ISE-Module.
ISE-Compressible Tube
ISE-Compressible Tube.JPG
ISE-Compressible Tube for Ion-Selective Electrode TPP+ and Ca2+.
ISE-Filling Syringe
ISE-Filling Syringe.JPG
ISE-Filling Syringe with needle for Ion-Selective Electrode TPP+ and Ca2+.
ISE-Inner Glass Electrode
ISE-Inner Glass Electrode.JPG
ISE-Inner Glass Electrode of ISE, with Ag/AgCl- and Pt-wire
ISE-Membrane Mounting Tool
ISA-Membrane Mounting Tool.JPG
ISE-Membrane Mounting Tool for Ion-Selective Electrode TPP+ and Ca2+. O2k-TPP+ ISE-Module: mounting tool included.
ISE-Membrane Seal
ISE-Membrane Seal.JPG
ISE-Membrane Seal for Ion-Selective Electrode TPP+ and Ca2+.
ISE-Service BoxISE-Service Box: for storage and shipment of the O2k-TPP+ ISE-Module or O2k-pH ISE-Module.
ISE-TPP+ Membranes
ISE-TPP+ Membranes.JPG
ISE-TPP+ Membranes, PVC, 4 mm diameter, box of 5 membranes.
ISS-Filter and Tubing
ISS-Filter and Tubing.JPG
ISS-Filter and Tubing, ISS-Integrated Suction System.
ISS-Integrated Suction System
ISS.jpg
ISS-Integrated Suction System: Suction pump with stainless steel housing, 2 liter waste bottle, filter and tubing; for siphoning off excess medium from the O2k-Stopper and for emptying the O2k-chambers. The ISS is included as a standard component of the O2k-FluoRespirometer. Media containing living cells or microorganisms, various poisons (inhibitors, uncouplers) and mixtures of proteins and substrates are safely disposed off in the 2-litre waste bottle.
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